Sweet cherries from Fundão possess antidiabetic potential and protect human erythrocytes against oxidative damage

Abstract

Cherries are one of the most appreciated summer fruits due to their attractive colour, sweet taste, high water content, low level of calories and composition in bioactive compounds which, in turn, are important to prevent some pathologies like diabetes, cardiovascular diseases and cancer. In this work we evaluated the phenolic profile and biological potential of 5 varieties of sweet cherries from Fundão region (Portugal) (Saco, Sweetheart, Satin, Maring and Hedelfinger). A total of 23 phenolic compounds were identified by LC-DAD and distributed by the several classes: 6 anthocyanins, 1 hydroxybenzoic acid, 8 hydroxycinnamic acids, 3 flavan-3-ols and 5 flavonols. Maring revealed higher contents in anthocyanins, while Hedelfinger was the richest in non-coloured phenolics. The antioxidant capacity was evaluated against DPPH and nitric oxide radicals. Hedelfinger was the most active against DPPH (IC=12.1μg/mL) and Maring against nitric oxide (IC=140.9μg/mL). Afterwards, antidiabetic capacity was evaluated through the inhibition of α-glucosidase activity, pointing Hedelfinger as the most active (IC=10.3μg/mL). The capacity of Saco extracts to inhibit the hemoglobin oxidation and the hemolysis of human erythrocytes was also evaluated. Both assays revealed a concentration-dependent inhibitory effect (IC=38.6μg/mL and IC=73.0μg/mL, respectively). The results obtained in this study allow us to conclude that sweet cherries possess a great biological potential, and further investigation should be done to promote commercialization and encourage its use in food supplements and in new pharmaceutical and nutraceutical applications.

Integral use of Argentinean Solanum betaceum red fruits as functional food ingredient to prevent metabolic syndrome: effect of in vitro simulated gastroduodenal digestion

“…μg GAE/mL) with major activity of pulps extracts (1 and 0.7 μg GAE/mL, before and after GD digestion). The activity was much higher than extracts of other fruits known for its capacity for the prevention of oxidation and cellular damage, such as quince (Cydonia oblonga) or sweet cherries (Prunus avium) (500 μg GAE/mL and 73 μg GAE/mL, respectively)(Magalhães et al, 2009;Gonçalves et al, 2017); and similar to orange chilto extracts (0.40-1.21 μg GAE/mL,Orqueda et al, 2017). All the extracts were good scavengers of reactive oxygen and nitrogen species (ROS/RNS), with IC 50 values between 4.2-78.3 μg GAE/mL.…”

Authentication of honeys from Caramulo region (Portugal): Pollen spectrum, physicochemical characteristics, mineral content, and phenolic profile

“…2.5.2 HPLC-DAD analysis. Twenty microliters of each sample were analyzed on a LC model Agilent 1260 system (Agilent, Santa Clara, CA, USA) using a Nucleosil R 100-5 C18 column (25.0 cm × 0.46 cm; 5 µm particle size waters; Macherey-Nagel), according to Gonçalves, Bento, Silva, and Silva (2017). Detection was achieved with an Agilent 1260 Infinity DAD using the ChemStation software supplied by Agilent Technologies (Waldbronn, Germany).…”

HPLC‐DAD Analysis, Antioxidant and Protective Effects of Tunisian Rhanterium suaveolens against Acetamiprid Induced Oxidative Stress on Mice Erythrocytes

“…It has been considered that CA contributes to alleviate oxidative stress in renal tissue of cadmium chloride in toxicated rats . In addition, Gonçalves et al . demonstrated that sweet cherries rich in flavonoids and phenolic acids, particularly p ‐coumaric acid, protect human erythrocytes from hemolysis.…”