“…Ultraviolet-visible spectrophotometry of untreated wastewater (here shown as WW) and wastewater after 1 week of operation of MFC, the pattern shows absorbance has increased from an initial 0.5 (brown shift) to 0.6 (blue shift), thereby removing mass concentration of contaminants in the process, the ideal absorbance for a standard solution (DM water) is also shown to peak at 1 [Colour figure can be viewed at wileyonlinelibrary.com] MFCs is to reduce the COD followed by the energy recovery as bioelectricity. For sewage, the major components that need to be treated before discharge include dissolved solids, suspended solids, alkalinity (as CO 3 1− ), sulfate (as SO 4 2− ), nitrates (NO 3 − ), phosphates (PO 4 3− ) with organic loads of sodium (Na 2+ ), calcium (Ca +2 ), magnesium (Mg), potassium (K + ), chlorine (Cl − ), ammonia (NH 3 1+ ), and trace quantities of manganese, copper, and zinc, all of which contributes to mg-COD/L of wastewater. 33 Studies have shown the various types of pathogens present in the wastewater at thermophilic temperatures (20-30°C) usually include viruses (Enteroviruses), bacteria (Salmonella spp., Vibrio cholerae, Shigella spp., and Fecal coliforms), protozoa (Entamoeba histolytica cysts), and Helminths.…”