Susceptibility status and Acetylcholinesterase (AChE) enzyme activity on <i>Aedes aegypti</i> L. (Diptera: Culicidae) Larvae against temephos

Rahayu, Resti; Fatimah, Gusti; Hasmiwati,

doi:10.5958/0974-4576.2020.00017.1

Cited by 2 publications

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“…Whereas, in another study area which is located in the same province, the population of Ae. aegypti has been considered resistant to temephos (0.012 mg LG 1 ) as reported in the village of Kapalo Koto and Gunung Pangilun in Padang 6 and Kanagarian Tanjung Bingkuang in Solok 17 with a mortality rate of 10, 61.7 and 78%, respectively. It was suggested that the difference in resistance status of Ae.…”

Section: Discussionmentioning

confidence: 97%

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Detection of Ace-1 Mutation in Temephos-Resistant Aedes aegypti L. in West Sumatra, Indonesia

Rahayu¹,

Melta²,

Hasmiwati³

2022

Pakistan J. of Biological Sciences

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Background and Objective: Dengue cases have increased while the spread is getting broader worldwide. Temephos has been frequently used to control the larvae of the Aedes aegypti L., the primary vector of dengue. The intensive use of this larvicide has given rise to resistance. This study aims to determine the susceptibility status of Ae. aegypti to temephos and examine the two mutations (F290V and F455W) that possibly occur in the Ace-1 gene of Ae. aegypti from Salido Sub-District, IV Jurai District, Pesisir Selatan Regency. Materials and Methods:The susceptibility test was performed referring to a standard method of the World Health Organization, followed by a molecular test (polymerase chain reaction) and sequencing. Results: The results showed that the larvae of Ae. aegypti have been tolerant to temephos (0.012 mg LG 1 ) with a percentage of larval mortality of 91.67%. The sequencing analysis in the Ace-1 gene revealed the absence of F290V and F455W mutation in temephos-resistant Ae. aegypti, but a point mutation was detected at codon 506. This mutation shifts the ACA codon to ACT, but still codes for the same amino acid, threonine. Conclusion: Our study indicates the presence of other resistance mechanisms in the major dengue vector of the Salido District. Implementation of the alternative population control strategy is required to prevent the temephos resistance further.

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Section: Discussionmentioning

confidence: 97%

“…Aedes aegypti is the primary vector of dengue 3 and since the 1970s, larvicide temephos has been widely used in controlling mosquito vectors in Indonesia 4 . Consequently, extensive and repeated use of the larvicide has manifested resistance development as reported in Indonesia [5][6][7] and also in several countries 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Detection of Ace-1 Mutation in Temephos-Resistant Aedes aegypti L. in West Sumatra, Indonesia

Rahayu¹,

Melta²,

Hasmiwati³

2022

Pakistan J. of Biological Sciences

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“…aegypti larval resistance occurs in the Ka Koto and Gu Pangilun populations. 8 Resistance to permethrin in Ae. aegypti was reported in Banjarmasin populations with low mortality due to mutation in the voltage-gated sodium channel gene.…”

Section: Introductionmentioning

confidence: 99%

The Susceptibility of Aedes aegypti In Dengue Endemic Areas, Tegal, Central Java Indonesia

Kresnadi

Amin

Ariq

et al. 2021

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Tegal district is a dengue-endemic area. One of the strategies to control Ae. aegypti is the use of insecticides. The determination of insecticide resistance in a dengue-endemic area is useful for supporting policies for Ae. aegypti control program. The aim of this study is to determine the susceptibility of Ae. aegypti in Tegal district, Central Java. Aedes aegypti larvae were collected from June to July 2018. Susceptibility bioassay of Ae. aegypti larvae against temephos and Ae. aegypti female against permethrin were conducted refers to the WHO protocol. The susceptibility of Ae. aegypti was interpreted based on WHO protocol as well. The mortality of Ae. aegypti larvae were at 0.025 ppm by 90%. The LC50 at 0.0005 ppm, and LC99 at 1.1037 ppm, respectively. The mortality rate of Ae. aegypti against permethrin was 26%. The LT50 at 6611.636 minutes, and LT99 at 5958807.272 minutes, respectively. The susceptibility of Ae. aegypti larvae were possible resistant but adult Ae. aegypti was resistant.

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Susceptibility status and Acetylcholinesterase (AChE) enzyme activity on Aedes aegypti L. (Diptera: Culicidae) Larvae against temephos

Cited by 2 publications

References 0 publications

Detection of Ace-1 Mutation in Temephos-Resistant Aedes aegypti L. in West Sumatra, Indonesia

Detection of Ace-1 Mutation in Temephos-Resistant Aedes aegypti L. in West Sumatra, Indonesia

The Susceptibility of Aedes aegypti In Dengue Endemic Areas, Tegal, Central Java Indonesia

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