The Susceptibility of Aedes aegypti In Dengue Endemic Areas, Tegal, Central Java Indonesia

Abstract

Tegal district is a dengue-endemic area. One of the strategies to control Ae. aegypti is the use of insecticides. The determination of insecticide resistance in a dengue-endemic area is useful for supporting policies for Ae. aegypti control program. The aim of this study is to determine the susceptibility of Ae. aegypti in Tegal district, Central Java. Aedes aegypti larvae were collected from June to July 2018. Susceptibility bioassay of Ae. aegypti larvae against temephos and Ae. aegypti female against permethrin were conducted refers to the WHO protocol. The susceptibility of Ae. aegypti was interpreted based on WHO protocol as well. The mortality of Ae. aegypti larvae were at 0.025 ppm by 90%. The LC50 at 0.0005 ppm, and LC99 at 1.1037 ppm, respectively. The mortality rate of Ae. aegypti against permethrin was 26%. The LT50 at 6611.636 minutes, and LT99 at 5958807.272 minutes, respectively. The susceptibility of Ae. aegypti larvae were possible resistant but adult Ae. aegypti was resistant.

Uji Resistensi Vektor Demam Berdarah Dengue (DBD) Terhadap Insektisida Permethrin di Kota Binjai Provinsi Sumatera Utara Tahun 2022

“…Resistensi silang (cross resistance) adalah perkembangan resistensi pada populasi serangga termasuk nyamuk akibat penekanan secara selektif insektisida lain dengan mekanisme sama/target site sama, tetapi bukan dari satu kelompok insektisida. 10 Resistensi vektor DBD terhadap Permethrin juga terjadi di beberapa daerah di Indonesia, yaitu di Jakarta 11 , Banjarmasin 12 , Makassar 13 , Denpasar 14 , Kabupaten Purbalingga 15 , Desa Pangkah Kabupaten Tegal 16 dan Kelurahan Sorosutan Kota Yogyakarta. 17 Salah satu cara untuk mengetahui status kerentanan atau resistensi nyamuk terhadap suatu insektisida adalah dengan menggunakan metode CDC bottle bioassay.…”

“…Resistensi 16 Uji kerentanan yang dilakukan pada populasi Ae. aegypti dari Kabupaten Purbalingga juga menunjukkan adanya resistensi terhadap permethrin dengan kematian nyamuk setelah 24 jam sebesar 0%.…”

Insecticide resistance of Aedes aegypti in Indonesia: a systematic review

“…Genetic, biological-ecological, and operational variables, circumstances for quick or slow resistance to emerge depending on how long and how frequently the pesticide is used, the number of mosquito breeding sites supplied with the treatment, and the incorrect dose are all potential causes of resistance [64], [65]. Some areas that experience resistance in Pesisir Selatan Regency, West Sumatra Province; this is due to the use of insecticides to eradicate mosquito nests coupled with the continuous use of pesticides as pesticides in agriculture [46], and it is present in endemic regions that have also seen temephos resistance in Kulon Progo Regency, Special Province of Yogyakarta (D.I Yogyakarta) [45] in Tegal Regency, Central Java Province [66]. The WHO-established criteria for resistance in these locations, which call for 80% mortality at a concentration of 0.02 ppm for both endemic and sporadic areas, are met [45].…”