Survey of Extended-Spectrum β-Lactamases in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae : Prevalence of TEM-52 in Korea

Abstract

Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum β-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain produced SHV-12, 1 strain produced a CMY-1-like enzyme, and 1 strain expressed SHV-2a and a CMY-1-like enzyme. Another two strains for which the MICs of ceftazidime and cefoxitin were high, were probable AmpC enzyme hyperproducers. Because of the high prevalence of TEM-52 in E. coli isolates, we further investigated the TEM-type ESBLs produced by Klebsiella pneumoniae in order to observe the distribution of TEM-52 enzymes among Enterobacteriaceae in Korea. All TEM enzymes produced by 12 strains of K. pneumoniae were identified as TEM-52. To evaluate the genetic relatedness among the organisms, ribotyping of TEM-52-producingE. coli and K. pneumoniae was performed. The ribotyping profiles of the organisms showed similar but clearly different patterns. In conclusion, TEM-52 is the most prevalent TEM-type ESBL in Korea.

Molecular diversity of Proteus mirabilis isolates producing extended-spectrum β-lactamases in a French university hospital

“…In contrast, the TEM-24 plasmid was associated with different b-lactam resistance markers. The results agreed with those reported previously concerning TEM-92, TEM-52 and TEM-24, which are encoded by 50-, 70-and 85-kb plasmids, respectively [10,23], although Pai et al [31] observed a great variability in plasmid size (between 71 and 100 kb) among TEM-52-producing E. coli and K. pneumoniae isolates from Korea. TEM-24 is produced predominantly by strains of Ent.…”

“…A TEM-92-producing P. mirabilis strain was first isolated in a French hospital in 1998 [14], and it was during the same year that a P. mirabilis isolate producing TEM-92 was identified in our laboratory. TEM-52 has been reported previously in K. pneumoniae [23,31], and subsequently in P. mirabilis isolates from Italy [14]. The first P. mirabilis isolate producing TEM-52 in our hospital was isolated in January 1999.…”

CTX-M and SHV-12 β-lactamases are the most common extended-spectrum enzymes in clinical isolates ofEscherichia coliandKlebsiella pneumoniaecollected from 3 university hospitals within Korea

“…Among gram-negative pathogens in Korea, the incidence of resistance to extended-spectrum b-lactam antibiotics is becoming an ever-increasing problem and rapid increase in ESBL-producing Escherichia coli and Klebsiella pneumoniae has been reported [6][7][8][9]. Although, the most commonly identified ESBLs in Korea are TEM-52, SHV-2a and SHV-12 [6][7][8][9], recent report suggests dissemination of CTX-M-14 in Korea [10]. Indeed, we here report the dissemination of CTX-M enzymes, such as CTX-M-3, CTX-M-9, CTX-M-15, and CTX-M-14, among the isolates of E. coli and Klebsiella spp.…”

Multidrug resistance in Gram-negative bacteria that produce extended-spectrum β-lactamases (ESBLs)

“…[76], 0–4% of E. coli isolates reported in European studies were resistant to ceftazidime, a rate similar to the 3% prevalence of ceftazidime resistance reported from ICUs in the USA. In Japan, in a recent survey of 196 institutions across the country, only < 0.1% of E. coli and 0.3% of K. pneumoniae strains expressed an ESBL, whereas corresponding figures for ESBL production in E. coli and K. pneumoniae from Korea, Taiwan and Hong Kong were 4.8%, 8.5% and 12%, respectively [77–80].…”