Molecular diversity of Proteus mirabilis isolates producing extended-spectrum β-lactamases in a French university hospital

Biendo, M.; Thomas, D.; Laurans, G.; Hamdad-Daoudi, Farida; Canarelli, B.; Rousseau, Florence; Castelain, Sandrine; Eb, F.

doi:10.1111/j.1469-0691.2005.01147.x

Cited by 18 publications

(14 citation statements)

References 24 publications

(39 reference statements)

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“…[37, 38, 41, 45, 46] The distribution of ESBL type varies geographically; TEM-type enzymes are the most common ESBLs in some areas. [8, 40, 47, 48] According to one Korean study of ESBL-producing P. mirabilis isolates in 2005, most ESBL producers possessed the bla TEM gene, followed by bla CTX-M . [16] In this study, of the 14 isolates from the ESBL-producing group, 78.5% (11/14) produced CTX-M, 50% (7/14) produced TEM, and 49% (6/14) produced both CTX-M and TEM; CTX-M type and TEM-type enzymes were also predominant in this study.…”

Section: Discussionmentioning

confidence: 99%

The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea

et al. 2017

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BackgroundThe incidence of Proteus mirabilis antimicrobial resistance, especially that mediated by extended-spectrum β-lactamases (ESBLs), has increased. We investigated the impact of ESBL production on the mortality of patients with P. mirabilis bacteremia in Korea.MethodsPatients diagnosed with P. mirabilis bacteremia between November 2005 and December 2013 at a 2000-bed tertiary care center in South Korea were included in this study. Phenotypic and molecular analyses were performed to assess ESBL expression. Characteristics and treatment outcomes were investigated among ESBL-producing and non-ESBL-producing P. mirabilis bacteremia groups. A multivariate analysis of 28-day mortality rates was performed to evaluate the independent impact of ESBLs.ResultsAmong 62 P. mirabilis isolates from 62 patients, 14 expressed ESBLs (CTX-M, 2; TEM, 5; both, 6; other, 1), and the 28-day mortality rate of the 62 patients was 17.74%. No clinical factor was significantly associated with ESBL production. The 28-day mortality rate in the ESBL-producing group was significantly higher than that in the non-ESBL-producing group (50% vs. 8.3%, p = 0.001). A multivariate analysis showed that ESBL production (odds ratio [OR], 11.53, 95% confidence interval [CI], 2.11–63.05, p = 0.005) was independently associated with the 28-day mortality rate in patients with P. mirabilis bacteremia.ConclusionsESBL production is significantly associated with mortality in patients with bacteremia caused by P. mirabilis. Rapid detection of ESBL expression and prompt appropriate antimicrobial therapy are required to reduce mortality caused by P. mirabilis bacteremia.

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Section: Discussionmentioning

confidence: 99%

The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea

et al. 2017

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“…A diversity of ESBLs such as TEM-derived mutants was reported to be produced by P. mirabilis in Europe (Bonnet et al, 1999;Luzzaro et al, 2001;Biendo et al, 2005). CTX-M-2-, CTX-M-3-, CTX-M-13-and CTX-M-14-producing P. mirabilis has been reported in Hong Kong, Taiwan and Japan (Nagano et al, 2003;Ho et al, 2005;Wu et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…However, occurrences of the species that are resistant to b-lactams, mediated by the production of acquired blactamases, have been reported recently in Europe and the US . Since the 1990s, P. mirabilis producing TEM-derived ESBLs (TEM-15, TEM-24, TEM-52 and TEM-66) has been reported (Bonnet et al, 1999;Luzzaro et al, 2001;Biendo et al, 2005). Non-TEM-derived ESBLs such as VEB-1 (Kim et al, 2004), CTX-M b-lactamases (CTX-M-1, CTX-M-2, CTX-M-13 and CTX-M-31) (Ho et al, 2005;Karapavlidou et al, 2005) and plasmid-borne AmpC blactamases (CMY-2 and CMY-4) (Verdet et al, 1998;Park et al, 2006) have also been reported infrequently.…”

Section: Introductionmentioning

confidence: 99%

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum b-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to .256 mg ml "1 ) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 b-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 blactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla CTX-M-2 isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla CTX-M-2 . These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

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“…The molecular epidemiology of ESBL-encoding genes in P. mirabilis isolated from humans varies widely between geographical regions. TEM types, especially TEM-52 and TEM-92, dominate in France and Italy (44,45), while CTX-M types are common in Israel (CTX-M-2), Poland (CTX-M-3), Japan (CTX-M-2), South Korea (CTX-M-14), and Argentina (CTX-M-2) (41,42,(46)(47)(48). Providencia species producing ESBL types other than CTX-M-2 have only been reported on a few occasions (49).…”

Section: Discussionmentioning

confidence: 99%

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

Hansen

Bortolaia

Damborg

et al. 2014

Appl Environ Microbiol

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, resulting in 98 isolates identified by matrixassisted laser desorption ionization-time of flight mass spectrometry and tested for bla CTX-M . CTX-M-positive isolates (n ‫؍‬ 86) were typed by pulsed-field gel electrophoresis (PFGE). Plasmids harboring bla CTX-M were characterized in 22 representative isolates by replicon typing and restriction fragment length polymorphism. Based on the PFGE results, all individuals shed unrelated CTX-M-14-producing E. coli strains during the course of life. Concomitant shedding of CTX-M-2/97-producing Proteus mirabilis or Providencia rettgeri was observed in two sows and two offspring. At least two genetically unrelated CTX-M-producing E. coli strains were isolated from approximately one-fourth of the samples, with remarkable differences between isolates obtained by enrichment and direct plating. A clear decrease in strain diversity was observed after weaning. Dissemination of bla CTX-M-14 within the farm was attributed to horizontal transfer of an IncK plasmid that did not carry additional resistance genes and persisted in the absence of antimicrobial selective pressure. Assessment of strain diversity was shown to be influenced by the production stage from which samples were collected, as well as by the isolation method, providing useful information for the design and interpretation of future epidemiological studies of CTX-M-producing Enterobacteriaceae in pig farms.

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Molecular diversity of Proteus mirabilis isolates producing extended-spectrum β-lactamases in a French university hospital

Cited by 18 publications

References 24 publications

The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea

The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Strain Diversity of CTX-M-Producing Enterobacteriaceae in Individual Pigs: Insights into the Dynamics of Shedding during the Production Cycle

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