Surface modification of poly(aryl ether ether ketone) (PEEK) film by covalent coupling of amines and amino acids through a spacer arm

Phosphonic Acid Monolayers for Binding of Bioactive Molecules to Titanium Surfaces

“…The activity of the surfaces was tested using trifluoroethylamine (TFEA) hydrochloride (similar to [27]). The activated samples were immersed in a 0.1 M solution of TFEA in phosphate buffered saline (PBS, pH=7.4) while shaking for 15 h at room temperature.…”

Synthesis and evaluation of RGD peptidomimetics aimed at surface bioderivatization of polymer substrates

“…The typical features in the 1 H NMR spectra of the tested compounds (unprotected derivatives in D 2 O) are as follows: (a) the three aromatic protons of the tyrosine backbone give respectively a ®ne doublet at 7.3±7.5 d (J $ 2 Hz), a doublet of doublet at 7.1±7.2 d (J $ 2 Hz and 8.7 Hz) and a doublet at 7.0±7.1 d (J $ 8.7 Hz); (b) the proton of the tyrosine a-CH group is a doublet of doublet at 4.6±4.8 d (J $ 5 and 9.5 Hz); (c) the protons of the tyrosine b-CH 2 group appear to be non-equivalent, giving a ABX pattern centred at 2.95±3.00 d and 3.25±3.30 d (JAB $ 14 Hz). In the 13 C NMR spectra (D 2 O), (a) the carbon atom of the CF 3 XPS tag is visible at 118 ppm (Q); (b) the three substituted carbon atoms of the tyrosine aromatic ring give lines at 153 ppm, 131 ppm and 127 ppm, corresponding to carbons linked to oxygen, nitrogen and carbon respectively; (c) the a and b carbons of the aliphatic tyrosine chain appear at 57 ppm and 38 ppm, respectively.…”

“…Complete deprotection of the ester, guanidino and amino functions was realized, as usual, by treatment with tri¯uoroacetic acid at room temperature; compound 16b was quantitatively recovered (Scheme 1). In the 1 H NMR spectrum, the three methylene protons of the anchorage-arm give multiplets at 2.13 d, 3.16 d and 4.16 d; the corresponding 13 C NMR lines are found at 28.9 ppm, 39.6 ppm and 68.5 ppm.…”

“…The IR spectra were taken with a Perkin± Elmer 600 instrument or with a Bio-Rad FTS 135 instrument, and calibrated with polystyrene (1601 cm À1 ). The 1 H and 13 C NMR spectra were recorded on Varian Gemini 300 (at 300 MHz for proton and 75 MHz for carbon), or Bruker AM-500 spectrometers (at 500 MHz for proton and 125 MHz for carbon); the chemical shifts are reported in ppm (d) down®eld from tetramethylsilane (internal standard), or sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS) for the spectra recorded in D 2 O. The atom numbering used for the description of the spectra is shown in the Figure 7; the attributions were established by selective decoupling experiments.…”

Covalent attachment of cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) to poly(etheretherketone) surface by tailored silanization layers technique

“…To further improve the biocompatibility of PEEK, many chemical and physical surface modification methods such as electron deposition titanium technique, thermal plasma spray deposition hydroxyapatite, and wet-chemistry method are in use [9][10][11]. The Arg-Gly-Asp (RGD) sequence and RGD-containing peptides have been found to promote cell adhesion, growth, differentiation and proliferation [12].…”