Surface immobilization of poly(ethylene oxide): Structure and properties

Gong, X. G.; Dai, Liming; Griesser, Hans J.; Mau, Albert W. H.

doi:10.1002/1099-0488(20000901)38:17<2323::aid-polb120>3.0.co;2-6

Cited by 63 publications

(49 citation statements)

References 43 publications

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“…3,8 Among them, the grafting of phosphorylcholine containing moieties 10 or incorporation of poly(ethylene oxide) chains to the hydrophilic pHEMA have been reported to reduce protein fouling. 11,12 Recently, an alternative approach to modify surface properties of the pHEMA based hydrogel has been proposed. 13 According to this method, PEG coupled monomer could be introduced with a desired density by adjusting the concentration of PEG carrying co-monomer in the polymer structures.…”

Section: Introductioncontrasting

confidence: 99%

Preparation and characterization of poly(hydroxyethyl methacrylate-co -poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

et al. 2011

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This study examined the effects of the surface properties of the materials, such as the hydroxyl, methyl and amino groups, on rat bone marrow derived Mesenchymal Stem Cell (MSC) seeding. A series of hydrogels were prepared in film form using 2-hydroxyethyl methacrylate (pHEMA), poly(ethyleneglycol) methacrylate (PEG-MA), and/or hydroxypropyl-chitosan (HPC). The physicochemical properties of these hydrogel films, such as water content, functional groups, contact angle, surface energy and thermal properties were affected by the composition of the materials. The ability of the MSCs to form colonies, as well as their viability on these materials were also analyzed. The water content of the hydrogel films increased with increasing PEG-MA and HPC ratio in the hydrogel. Contact angle measurements of the surface of the hydrogel films demonstrated that all the materials gave rise to a significantly hydrophilic surface compared to pure pHEMA. The blood protein interactions and platelet adhesion were reduced significantly on the surface of the materials upon the incorporation of PEG-MA compared to the control pure pHEMA and vice versa for HPC. The ability of the MSCs to adhere and form colonies on these materials was also analyzed. The results showed that these materials are suitable candidates to isolate and expand MSCs.

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Section: Introductioncontrasting

confidence: 99%

Preparation and characterization of poly(hydroxyethyl methacrylate-co -poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

et al. 2011

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“…21 The appearance of nitrogen peak and the increase in the relative amount of C-O, with concurrent decrease in the relative amount of C=O, are indication for the successful coupling of the PEG to the aldehyde surface via reductive amination. 59 The chemical surface reaction conducted on silicon substrates showed identical trend.…”

Section: X-ray Photoelectron Spectroscopy (Xps)supporting

confidence: 88%

“…The peaks as a shift of À1.5 eV and À3 eV from the C-C peak is the characteristic PEG coupling C-O peak and aldehyde C=O peak, respectively. 21 Spectral analysis was performed using the software proved with the XPS instrument. Percents of atomic composition and atomic ratios were corrected using sensitivity factor incorporated in the software.…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

Surface-Bound Proteins with Preserved Functionality

et al. 2009

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Abstract-Biocompatibility of materials strongly depends on their surface properties. Therefore, surface derivatization in a controllable manner provides means for achieving interfaces essential for a broad range of chemical, biological, and medical applications. Bioactive interfaces, while manifesting the activity for which they are designed, should suppress all nonspecific interaction between the supporting substrates and the surrounding media. This article describes a procedure for chemical derivatization of glass and silicon surfaces with polyethylene glycol (PEG) layers covalently functionalized with proteins. While the proteins introduce the functionality to the surfaces, the PEGs provide resistance against nonspecific interactions. For formation of aldehyde-functionalized surfaces, we coated the substrates with acetals (i.e., protected aldehydes). To avoid deterioration of the surfaces, we did not use strong mineral acids for the deprotection of the aldehydes. Instead, we used a relatively weak Lewis acid for conversion of the acetals into aldehydes. Introduction of a,x-bifunctional polymers into the PEG layers, bound to the aldehydes, allowed us to covalently attach green fluorescent protein and bovine carbonic anhydrase to the surfaces. Spectroscopic studies indicated that the surface-bound proteins preserve their functionalities. The surface concentrations of the proteins, however, did not manifest linear proportionality to the molar fractions of the bifunctional PEGs used for the coatings. This finding suggests that surface-loading ratios cannot be directly predicted from the compositions of the solutions of competing reagents used for chemical derivatization.

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“…[22][23][24][25][26][27] The covalent grafting of PEG onto a variety of substrates, including silicon, 24,28 polyurethane-urea, 29 glass, 30,31 fluorinated ethylene-propylene copolymers, 32 and polysulfone membranes, 33 has been reported along with a quite satisfactory protein-repellent effect. It may be anticipated that the proteinrepellent properties of PEG would prevent an extracellular matrix from being formed and, thus, cells from adhering.…”

Section: Introductionmentioning

confidence: 99%

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

et al. 2007

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Cataract surgery is a routine ophthalmologic intervention resulting in replacement of the opacified natural lens by a polymeric intraocular lens (IOL). A main postoperative complication, as a result of protein adsorption and lens epithelial cell (LEC) adhesion, growth, and proliferation, is the secondary cataract, referred to as posterior capsular opacification (PCO). To avoid PCO formation, a poly(ethylene glycol) (PEG) chemical coating was created on the surface of hydrogel IOLs. Attenuated total reflectance Fourier transform infrared spectroscopy, “captive bubble” and “water droplet” contact angle measurements, and atomic force microscopy analyses proved the covalent grafting of the PEG chains on the IOL surface while keeping unchanged the optical properties of the initial material. A strong decrease of protein adsorption and cell adhesion depending on the molar mass of the grafted PEG (1100, 2000, and 5000 g/mol) was observed by performing the relevant in vitro tests with green fluorescent protein and LECs, respectively. Thus, the study provides a facile method for developing materials with nonfouling properties, particularly IOLs.

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Surface immobilization of poly(ethylene oxide): Structure and properties

Cited by 63 publications

References 43 publications

Preparation and characterization of poly(hydroxyethyl methacrylate-co -poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

Preparation and characterization of poly(hydroxyethyl methacrylate-co -poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

Surface-Bound Proteins with Preserved Functionality

Improved Performances of Intraocular Lenses by Poly(ethylene glycol) Chemical Coatings

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