Preparation and characterization of poly(hydroxyethyl methacrylate-co -poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

Abstract

This study examined the effects of the surface properties of the materials, such as the hydroxyl, methyl and amino groups, on rat bone marrow derived Mesenchymal Stem Cell (MSC) seeding. A series of hydrogels were prepared in film form using 2-hydroxyethyl methacrylate (pHEMA), poly(ethyleneglycol) methacrylate (PEG-MA), and/or hydroxypropyl-chitosan (HPC). The physicochemical properties of these hydrogel films, such as water content, functional groups, contact angle, surface energy and thermal properties were affected by the composition of the materials. The ability of the MSCs to form colonies, as well as their viability on these materials were also analyzed. The water content of the hydrogel films increased with increasing PEG-MA and HPC ratio in the hydrogel. Contact angle measurements of the surface of the hydrogel films demonstrated that all the materials gave rise to a significantly hydrophilic surface compared to pure pHEMA. The blood protein interactions and platelet adhesion were reduced significantly on the surface of the materials upon the incorporation of PEG-MA compared to the control pure pHEMA and vice versa for HPC. The ability of the MSCs to adhere and form colonies on these materials was also analyzed. The results showed that these materials are suitable candidates to isolate and expand MSCs.

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

“…Thus, polymer modification techniques have become a new and general way to functionalize polymers, and their potential applications in the fields of biomedical materials design [38][39][40]. Previously, we have reported a biocompatible film composed of poly(hydroxyethyl methacrylate-poly(ethyleneglycolmethacrylate)/hydroxypropyl-chitosan for adhesion of rat mesenchymal stem cells using a interpenetrating technique [4]. In the present work, a bioactive film based on poly(hydroxyethyl methacrylateglycidylmethacrylate) was investigated for modification.…”

“…The reactive epoxy group carrying p(HEMA-GMA) films were modified to induce cell responses, in particular \NH 2 , \CH 3 , \SO 3 H, aromatic groups substituted \OH and \COOH, L-histidine, L-arginine, L-phenylalanine, L-glutamic acid, L-tryptophan, have been incorporated onto p(HEMA-GMA) surface via epoxy groups of the functional polymer without altering its bulk properties. Our purpose was to evaluate the suitability of these biomaterials in MSCs' attachment and maintenance since MSCs have been shown to have very important roles in regenerative medicine and tissue engineering [4]. Using stem cells as a therapeutic agent has been receiving tremendous interest as it provides opportunities to combat with debilitating diseases.…”

“…The properties of hydrogels such as the functional groups, chemical composition, hydrophilic-hydrophobic balance, surface morphology, and presence of adherent proteins are essential for the regulation of cellular interactions. Therefore, the performance of a hydrogel material relies highly upon the properties of the boundaries in tissue engineering applications because the interactions between the hydrogel materials and environments occur mainly on its surfaces [1][2][3][4]. The hydroxyethyl methacrylate (pHEMA) hydrogel possesses good biocompatibility as evidenced by its large scale use in biomedical and biotechnological applications such as contact lenses, drug delivery systems, and soft tissue prosthesis [5][6][7][8][9].…”

Pharmacokinetics and biodegradation performance of a hydroxypropyl chitosan derivative

“…In addition, HP-chitosan derivatives have been employed as composite hydrogel for corneal endothelium reconstruction and adhesion scaffold of mesenchymal stem cells in tissue engineering (Gao et al, 2008;Wang et al, 2009;Liang et al, 2011). Recently, more and more experimental results have been reported on the potential applications and exploitations of CM-chitosan and HP-chitosan in absorbable biomaterial field (Ling et al, 2008;Bayramoglu et al, 2011;Riva et al, 2011).…”

Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells

“…The natural next step in such a study would be to embed CNTs while preserving their alignment in a biodegradable matrix manufactured from chitosan, polylactic acid, etc. [40]. In this concern, our studies regarding the transfer of aligned CNT arrays to a polymer matrix are ongoing.…”