Surface-Bound Proteins with Preserved Functionality

Wan, Jiandi; Thomas, Marlon S.; Guthrie, Sean; Vullev, Valentine I.

doi:10.1007/s10439-009-9673-6

Cited by 32 publications

(38 citation statements)

References 84 publications

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“…Employing chemical reactions that proceed through small intermediates with quantitative yields, such as reductive amination, ensures sufficiently well-packed PEG layers. 47 The bio-inertness of the PEG coatings strongly depends on the length of the polymer chains. Our goal encompassed not only suppressing molecular-level nonspecific interactions -which can be achieved even with ethylene glycol oligomers (ie, with three to six repeating ethylene glycol units) -but also preventing nonspecific adhesion at mesoscopic (micron and sub-micron) levels.…”

Section: Discussionmentioning

confidence: 99%

Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs

Bahmani¹,

Lytle²,

Walker³

et al. 2013

IJN

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Near-infrared nanoconstructs present a potentially effective platform for site-specific and deep tissue optical imaging and phototherapy. We have engineered a polymeric nanocapsule composed of polyallylamine hydrochloride (PAH) chains cross-linked with sodium phosphate and doped with indocyanine green (ICG) toward such endeavors. The ICG-doped nanocapsules were coated covalently with polyethylene glycol (5000 daltons) through reductive amination. We administrated the constructs by tail vein injection to healthy mice. To characterize the biodistribution of the constructs, we performed in vivo quantitative fluorescence imaging and subsequently analyzed the various extracted organs. Our results suggest that encapsulation of ICG in these PEGylated constructs is an effective approach to prolong the circulation time of ICG and delay its hepatic accumulation. Increased bioavailability of ICG, due to encapsulation, offers the potential of extending the clinical applications of ICG, which are currently limited due to rapid elimination of ICG from the vasculature. Our results also indicate that PAH and ICG-doped nanocapsules (ICG-NCs) are not cytotoxic at the levels used in this study.

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Section: Discussionmentioning

confidence: 99%

Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs

Bahmani¹,

Lytle²,

Walker³

et al. 2013

IJN

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“…11,12 Interfacial charge transport and CT "hold" the key for improving energy-conversion efficiencies of materials and devices. 4,13,14 Molecular-level understanding of charge-transfer rectification, implemented with a rational control of surfaces and interfaces, 5,[15][16][17] provides the venues for such efficiency improvements. The magnitude of CT rectification for a single Aa residue is comparable with the rectification exerted by peptide helices comprising more than 10 amino acid residues.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired molecular electrets: bottom-up approach to energy materials and applications

2015

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Abstract. The diversity of life on Earth is made possible through an immense variety of proteins that stems from less than a couple of dozen native amino acids. Is it possible to achieve similar engineering freedom and precision to design electronic materials? What if a handful of nonnative residues with a wide range of characteristics could be rationally placed in sequences to form organic macromolecules with specifically targeted properties and functionalities? Referred to as molecular electrets, dipolar oligomers and polymers composed of non-native aromatic beta-amino acids, anthranilamides (Aa) provide venues for pursuing such possibilities. The electret molecular dipoles play a crucial role in rectifying charge transfer, e.g., enhancing charge separation and suppressing undesired charge recombination, which is essential for photovoltaics, photocatalysis, and other solar-energy applications. A set of a few Aa residues can serve as building blocks for molecular electrets with widely diverse electronic properties, presenting venues for bottom-up designs. We demonstrate how three substituents and structural permutations within an Aa residue widely alter its reduction potential. Paradigms of diversity in electronic properties, originating from a few changes within a basic molecular structure, illustrate the promising potentials of biological inspiration for energy science and engineering.

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“…Steady‐state emission measurements were conducted using a FluoroLog‐3 spectrofluorometer (Horiba‐Jobin‐Yvon) equipped with double‐grating monochromators and a TBX single‐photon‐counting detector. For the liquid samples, we used right‐angle settings, and for the solid‐film samples, the emission was recorded at small‐angle configuration 44…”

Section: Methodsmentioning

confidence: 99%

Photoinduced Electron Transfer Between Pyridine Coated Cadmium Selenide Quantum Dots and Single Sheet Graphene

Guo

Bao

Upadhyayula

et al. 2013

Adv Funct Materials

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Interest in graphene as a two‐dimensional quantum‐well material for energy applications and nanoelectronics has increased exponentially in the last few years. The recent advances in large‐area single‐sheet fabrication of pristine graphene have opened unexplored avenues for expanding from nano‐ to meso‐scale applications. The relatively low level of absorptivity and the short lifetimes of excitons of single‐sheet graphene suggest that it needs to be coupled with light sensitizers in order to explore its feasibility for photonic applications, such as solar‐energy conversion. Red‐emitting CdSe quantum dots are employed for photosensitizing single‐sheet graphene with areas of several square centimeters. Pyridine coating of the quantum dots not only enhances their adhesion to the graphene surface, but also provides good electronic coupling between the CdSe and the two‐dimensional carbon allotrope. Illumination of the quantum dots led to injection of n‐carrier in the graphene phase. Time‐resolved spectroscopy reveals three modes of photoinduced electron transfer between the quantum dots and the graphene occurring in the femtosecond and picosecond time‐domains. Transient absorption spectra provide evidence for photoinduced hole‐shift from the CdSe to the pyridine ligands, thereby polarizing the surface of the quantum dots. That is, photoinduced electrical polarization, which favors the simultaneous electron transfer from the CdSe to the graphene phase. These mechanistic insights into the photoinduced interfacial charge transfer have a promising potential to serve as guidelines for the design and development of composites of graphene and inorganic nanomaterials for solar‐energy conversion applications.

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Surface-Bound Proteins with Preserved Functionality

Cited by 32 publications

References 84 publications

Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs

Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs

Bioinspired molecular electrets: bottom-up approach to energy materials and applications

Photoinduced Electron Transfer Between Pyridine Coated Cadmium Selenide Quantum Dots and Single Sheet Graphene

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