Surface coatings with covalently attached anidulafungin and micafungin preventCandida albicansbiofilm formation

Candida albicans Can Survive Antifungal Surface Coatings on Surfaces with Microcone Topography

“…While it is unknown whether the binding solution of caspofungin prepared in this study (using PBS buffer) contained aggregates, surface data from XPS indicate a submonolayer of caspofungin surface binding, suggesting that, if any aggregate material was initially bound, it would have been dissociated after SDS washing. Indeed, using the stringent regime of SDS washing at 70 °C has been reported in earlier studies as an effective means of desorbing physisorbed surface-bound molecules; ,,− accordingly, it appears reasonable to surmise that caspofungin was covalently immobilized on the plasma polymer interlayer. , Time-of-flight secondary ion spectrometry (ToF-SIMS) analysis (Figure c) confirmed the presence of nitrogen-containing fragment ions on the plasma polymer interlayer after exposure to caspofungin solution, for example, at m / z 86, which corresponds to the C 4 H 8 NO + fragment ion ejected from immobilized caspofungin, in accord with earlier studies. ,, …”

“…While covalently attached antifungal drugs can achieve >99.999% reduction in biofilm formation on smooth flat material surfaces, 18,19 here, we show that the effectiveness is much reduced when applying such a coating onto a surface with microtopography. Using scanning electron microscopy (SEM), this was shown to be a result of fungal cells being able to survive and grow hyphal extensions if they attached to cones, thereby exposing a much reduced area of their cell wall to the lethal coating.…”

“…For example, antifungal surface coatings with covalently attached antimicrobial drugs were shown to be lethal to contacting yeast cells, capable of repelling challenges of 10 6 colony forming units per square centimeter, degrading cells on contact, and thus preventing biofilm formation. 18,19 Some natural and synthetic surfaces with sharp topographical microfeatures can also be lethal to attaching microbes, provided the spacing and aspect ratio of the features are sufficiently small for the cell membrane to be pierced. 29,30 The microcones of the present study are not capable of rupturing cell membranes; their purpose was, instead, to be a well-defined mimic of rough biomaterial surfaces.…”

“…Plasma polymerization and antifungal biomolecule immobilization were conducted using methods described in previous studies. 19,46 After caspofungin binding to the surface coating, a series of washing steps were performed to remove physisorbed molecules. The SDS washing steps included application of 400 μL of 0.2 M prewarmed SDS solution on each of the samples.…”

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

“…In prophylactic treatment of Galleria mellonella infected with C. albicans , these liposomal anidulafungin nanoparticles increased larvae survival beyond the increased survival noted with free drug treatment. Anidulafungin has previously been incorporated into poly(methyl methacrylate) bone cement (Rouse, Heijink, Steckelberg, & Patel, 2011) and covalently tethered to glass and silicon surface coatings (Naderi, Giles, Saboohi, Griesser, & Coad, 2019) to prevent fungal attachment and infections. To the best of our knowledge, this is the first report of a nanoparticle encapsulating anidulafungin for future use in antifungal drug delivery.…”