The nature of the
protein corona forming on biomaterial surfaces
can affect the performance of implanted devices. This study investigated
the role of surface chemistry and wettability on human serum-derived
protein corona formation on biomaterial surfaces and the subsequent
effects on the cellular innate immune response. Plasma polymerization,
a substrate-independent technique, was employed to create nanothin
coatings with four specific chemical functionalities and a spectrum
of surface charges and wettability. The amount and type of protein
adsorbed was strongly influenced by surface chemistry and wettability
but did not show any dependence on surface charge. An enhanced adsorption
of the dysopsonin albumin was observed on hydrophilic carboxyl surfaces
while high opsonin IgG2 adsorption was seen on hydrophobic hydrocarbon
surfaces. This in turn led to a distinct immune response from macrophages;
hydrophilic surfaces drove greater expression of anti-inflammatory
cytokines by macrophages, whilst surface hydrophobicity caused increased
production of proinflammatory signaling molecules. These findings
map out a unique relationship between surface chemistry, hydrophobicity,
protein corona formation, and subsequent cellular innate immune responses;
the potential outcomes of these studies may be employed to tailor
biomaterial surface modifications, to modulate serum protein adsorption
and to achieve the desirable innate immune response to implanted biomaterials
and devices.
Nanoparticles have become an important utility in many areas of medical treatment such as targeted drug and treatment delivery as well as imaging and diagnostics. These advances require a complete understanding of nanoparticles' fate once placed in the body. Upon exposure to blood, proteins adsorb onto the nanoparticles surface and form a protein corona, which determines the particles' biological fate. This study reports on the protein corona formation from blood serum and plasma on spherical and rod‐shaped nanoparticles. These two types of mesoporous silica nanoparticles have identical chemistry, porosity, surface potential, and size in the y‐dimension, one being a sphere and the other a rod shape. The results show a significantly larger amount of protein attaching from both plasma and serum on the rod‐like particles compared to the spheres. Interrogation of the protein corona by liquid chromatography–mass spectrometry reveals shape‐dependent differences in the adsorption of immunoglobulins and albumin proteins from both plasma and serum. This study points to the need for taking nanoparticle shape into consideration because it can have a significant impact on the fate and therapeutic potential of nanoparticles when placed in the body.
The importance of nanostructured surfaces in a range of technological and biological processes is welldocumented within literature, yet often ill-understood. Simple and reliable methods for the preparation of nanotextured surfaces are required to advance both fundamental understandings of nanoscale phenomena and our capacity to design nano-engineered materials for specific applications. Nanoengineered surfaces are, for instance, needed to shed light on the effect of nanostructures' size and density on immune cells cytokine production. In applied bioengineering, nanostructured artificial surfaces could be specifically tailored to enhance the osteo-integration of implants. This study presents a versatile, plasma polymer enabled method for the generation of surfaces with well-defined nanotopography and tailored outermost surface chemistry. This was achieved by finely controlling the covalent bonding of gold nanoparticles of desired size to plasma-deposited poly(methyloxazoline) interlayer deposited on the material substrate. An additional 5 nm thin polymer was deposited over the nanostructures providing a uniformly tailored outermost surface chemistry while preserving the topography. This rapid, versatile, substrate independent, and scalable strategy for the preparation of a well-defined nanotopography surface has promising prospects in many fields relying on surface engineering, including food and membrane technologies, biomaterial and environmental engineering, sensing, marine sciences, and even pollution control.
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