Supplementation of a western diet with golden kiwifruits (Actinidia chinensis var.'Hort 16A':) effects on biomarkers of oxidation damage and antioxidant protection

Abstract

BackgroundThe health positive effects of diets high in fruits and vegetables are generally not replicated in supplementation trials with isolated antioxidants and vitamins, and as a consequence the emphasis of chronic disease prevention has shifted to whole foods and whole food products.MethodsWe carried out a human intervention trial with the golden kiwifruit, Actinidia chinensis, measuring markers of antioxidant status, DNA stability, plasma lipids, and platelet aggregation. Our hypothesis was that supplementation of a normal diet with kiwifruits would have an effect on biomarkers of oxidative status. Healthy volunteers supplemented a normal diet with either one or two golden kiwifruits per day in a cross-over study lasting 2 × 4 weeks. Plasma levels of vitamin C, and carotenoids, and the ferric reducing activity of plasma (FRAP) were measured. Malondialdehyde was assessed as a biomarker of lipid oxidation. Effects on DNA damage in circulating lymphocytes were estimated using the comet assay with enzyme modification to measure specific lesions; another modification allowed estimation of DNA repair.ResultsPlasma vitamin C increased after supplementation as did resistance towards H2O2-induced DNA damage. Purine oxidation in lymphocyte DNA decreased significantly after one kiwifruit per day, pyrimidine oxidation decreased after two fruits per day. Neither DNA base excision nor nucleotide excision repair was influenced by kiwifruit consumption. Malondialdehyde was not affected, but plasma triglycerides decreased. Whole blood platelet aggregation was decreased by kiwifruit supplementation.ConclusionGolden kiwifruit consumption strengthens resistance towards endogenous oxidative damage.

The effect of consuming Palmaria palmata-enriched bread on inflammatory markers, antioxidant status, lipid profile and thyroid function in a randomised placebo-controlled intervention trial in healthy adults

“…Isolated lymphocytes were subsequently counted, resuspended in cryostorage media (90 % FBS, 10 % glycerol) and stored at −80 °C until analysis. The single cell gel electrophoresis assay was used to investigate the influence of the intervention on genotoxicity, anti-genotoxicity and oxidative purine damage as described by Brevick et al [29]. DNA damage was quantified using an epifluorescent microscope and Komet 3.0 image analysis software (Kinetic Imaging Ltd, Liverpool, UK).…”

Beneficios nutricionales y sanitarios asociados al consumo de kiwi

“…Se ha demostrado que el consumo regular tanto de kiwi verde como de la variedad amarilla aumenta significativamente la concentración plasmática de vitaminas antioxidantes, como la C (10,13,14), la E (10) y la de luteína/zeaxantina (10,13). Además, la capacidad antioxidante in vitro del kiwi, si se compara con la de otras frutas, puede considerarse como media-alta, dependiendo del tipo de ensayo realizado (11,12).…”

“…Por ejemplo, consumir kiwi verde Green ® con las comidas aumenta la capacidad antioxidante del plasma posprandial, lo que podría prevenir el estrés oxidativo justo después de las comidas (15 Cobertura de las ingestas recomendadas de energía y vitaminas con 100 g de porción comestible de kiwi verde y kiwi Sungold ® (5). rios durante 3-4 semanas mejora la resistencia del DNA de los linfocitos frente al daño oxidativo (14,16). Además, el consumo de 4 kiwis Sungold ® diarios durante 4 semanas se ha asociado con una disminución de la peroxidación lipídica (10).…”

Inhibitory effects of kiwifruit extract on human platelet aggregation and plasma angiotensin-converting enzyme activity

“…Kiwifruit contains very significant amounts of various phytochemicals, such as anthocyanidins and flavonols [27]. The common green kiwifruit, Actinidia deliciosa, has been used in several trials to examine effects on biomarkers relevant to CVD [4,[28][29][30]. Daily consumption of two or three kiwifruits reduced platelet aggregation response, blood pressure and plasma lipids [4,[28][29][30].…”