Supercritical fluid chromatography in pharmaceutical analysis

Simultaneous analysis of nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

“…The addition of small amounts of additives in mobile phase component B (MeOH), such as acids, bases or salts, was then tested in order to improve selectivity and enhance MS sensitivity [35]. Three additives (0.1% formic acid (v/v), 0.1% ammonium hydroxide (v/v) and 5 mM ammonium acetate) were investigated.…”

A sensitive, high‐throughput, and ecofriendly method for the determination of lumefantrine, artemether, and its active metabolite dihydroartemisinin by supercritical fluid chromatography and tandem mass spectrometry

“…The ACQUITY Ultra‐Performance Convergence Chromatography (UPC 2 ) system, which applies convergence chromatography to enable superior performance by using inexpensive supercritical CO 2 as a green mobile phase, was commercially introduced by Waters in 2012 . It is particularly useful for thermally labile compounds such as artemisinin due to the ability of supercritical CO 2 to function at low temperatures.…”

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

“…GrandGuillaume Perrenoud et al states that basic compounds often give poor chromatographic performances and can suffer strong peak shape distortions, including tailing, fronting, splitting and shouldering [27]. However, recently Desfontaine et al investigated the use of novel stationary phases for the achiral analysis of basic drugs in SFC [28]. They for example showed that more than 90% of the studied compounds showed non-symmetrical peak shapes in pure CO 2 /methanol mobile phase without additive.…”