Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Forss, Erik; Haupt, Dan; Stålberg, Olle; Enmark, Martin; Samuelsson, Jörgen; Fornstedt, Torgny

doi:10.1016/j.chroma.2017.03.077

Cited by 32 publications

(17 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Variation of the modifier composition causes the changes in the mobile phase solvation strength, and variation of the modifier concentration is the main reason causing the changes of the analyte retention 10,65 . In most cases, increasing the modifier fraction reduces the analyte retention; however, if too much of the modifier is used, super(sub)critical conditions of the mobile phase cannot be maintained 66 …”

Section: Packed Column Supercritical Fluid Chromatographymentioning

confidence: 99%

A review of retention mechanism studies for packed column supercritical fluid chromatography

Si-Hung

Bamba

2021

Analytical Science Advances

View full text Add to dashboard Cite

The packed column supercritical fluid chromatography has risen as a promising alternative separation technique to the conventional liquid chromatography and gas chromatography. Although the packed column supercritical fluid chromatography has many advantages compared to other chromatographic techniques, its separation mechanism is not fully understood due to the complex combination effects of many chromatographic parameters on separation quality and the lacking of global strategies for studying separation mechanisms. This review aims to provide recent information regarding the chromatographic behaviors and the effects of the parameters on the separation, discuss the results, and point out the remaining bottlenecks in the packed column supercritical fluid chromatography retention mechanism studies.

show abstract

Section: Packed Column Supercritical Fluid Chromatographymentioning

confidence: 99%

A review of retention mechanism studies for packed column supercritical fluid chromatography

Si-Hung

Bamba

2021

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…The effects of the nature and concentration of the alcohol modifier, the temperature and the structures of the analytes and selectors on retention and selectivity were studied. The problem of calculating the real operational conditions in SFC was also recently reported . In our case, for the sake of operational simplicity, only the numerical settings of the SFC system were varied.…”

Section: Introductionmentioning

confidence: 98%

“…The possible effects of amines as mobile phase additives on the property and the thickness of the adsorbed layer formed on the surface of the stationary phase were also described . It was demonstrated that by applying a polysaccharide‐type CSP for the enantiomer separation of basic drugs, the NP retention and selectivity rules may not apply to SFC conditions due to the fundamentally different operational properties of the mobile phases .…”

Section: Introductionmentioning

confidence: 99%

High‐performance liquid chromatographic and subcritical fluid chromatographic separation of α‐arylated ß‐carboline, N‐alkylated tetrahydroisoquinolines and their bioisosteres on polysaccharide‐based chiral stationary phases

Bajtai

Lajkó

Németi

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

New, pharmacologically interesting chiral amino compounds, namely, stereoisomers of α‐hydroxynaphthyl‐ß‐carboline, benz[d]azepine and benz[c]azepine analogs as well as N‐α‐hydroxynaphthylbenzyl‐substituted isoquinolines were enantioseparated by high‐performance liquid chromatographic and subcritical fluid chromatographic methods on polysaccharide‐based chiral stationary phases. Separation of the stereoisomers was optimized in both subcritical fluid chromatography and normal phase liquid chromatographic modes by investigating the effects of the composition of the bulk solvent, temperature, and the structures of the analytes and selectors. Both normal phase liquid chromatography and subcritical fluid chromatography exhibited satisfactory performance, albeit with somewhat different effectiveness in the separation of the stereoisomers studied. The optimized methods offer the possibility to apply preparative‐scale separations thereby enabling further pharmacological investigations of the enantiomers.

show abstract

“…It is possible that, because SFC uses supercritical fluids as the mobile phase, selectivity will differ from that of HPLC. The selectivity of SFC can be controlled by changing the mobile phase composition, temperature, and pressure [2,3,4,5,6], but the stationary phase most significantly affects selectivity. In addition to the number of stationary phases specially designed for SFC, stationary phases used in HPLC procedures are also used for SFC, making it is difficult to select the optimal stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Hirose¹,

Keck²,

Izumi

et al. 2019

Molecules

View full text Add to dashboard Cite

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

show abstract

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Cited by 32 publications

References 39 publications

A review of retention mechanism studies for packed column supercritical fluid chromatography

A review of retention mechanism studies for packed column supercritical fluid chromatography

High‐performance liquid chromatographic and subcritical fluid chromatographic separation of α‐arylated ß‐carboline, N‐alkylated tetrahydroisoquinolines and their bioisosteres on polysaccharide‐based chiral stationary phases

Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Contact Info

Product

Resources

About