Simultaneous analysis of nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Supercritical CO2 Extraction and Identification of Ginsenosides in Russian and North Korean Ginseng by HPLC with Tandem Mass Spectrometry

“…Thus, exhaustive supercritical extraction using ethanol could be used to obtain ginsenoside extracts from the ginseng crown. As well as using methanol as a co-solvent, it allows one to achieve good qualitative and quantitative indicators, while the use of ethanol in the food industry is more preferable compared to methanol [19,32]. We isolated 21 ginsenosides, which is a quarter more than previously isolated under supercritical extraction conditions; we attribute this to two factors.…”

“…Many researchers have reported this synergistic effect in previous studies using supercritical CO 2 extraction [30,31]. In spite of the fact that the co-solvent methanol is most often used for qualitative analysis, we chose ethanol as the co-solvent since extraction in a closed-cycle plant was carried out in this work, and the main idea was not just a qualitative analysis, but an analysis of the applicability of technology for the food industry [19,32,33]. Moreover, we decided to use the minimum amount of co-solvent, which gave a significant increase in the yield of the product.…”

“…We isolated 21 ginsenosides, which is a quarter more than previously isolated under supercritical extraction conditions; we attribute this to two factors. The first is that the conditions for the growth of ginseng in the northern regions are more contrasting in temperature, and the second, that we used pressures of up to 500 bar, while previously the researchers were limited to 220 bars [32,33].…”

Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

“…Consequently, UHPSFC has increasingly developed to a valuable alternative to organic solvent based reversed phase chromatography in modern natural product analysis [13][14][15]. The main potential of UHPSFC is seen as analytical alternative for the assessment of nonpolar and moderate polar plant constituent; its applicability to more polar compounds is still less researched [13][14][15][16][17][18][19][20][21][22]. A second limitation in current literature is the focus of most validated, quantitative applications on single secondary metabolite classes.…”

Quantitative determination of major alkaloids in Cinchona bark by Supercritical Fluid Chromatography

“…In the past the technique was predominantly used for the analysis of non-polar compounds like fatty acids [18], triglycerides [19] or carotenoids [20]. Recent publications point to a much wider range of possible applications also including polar natural products [21,22] and alkaloids [23–25]. Therefore, we attempted to separate and quantify the alkaloids in Cinchona bark by SFC.…”