Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells

Abstract

BackgroundAdipose-derived stem cells (ASCs) have been identified as a population of multipotent cells with promising applications in tissue engineering and regenerative medicine. ASCs are abundant in fat tissue, which can be safely harvested through the minimally invasive procedure of liposuction. However, there exist a variety of different harvesting methods, with unclear impact on ASC regenerative potential. The aim of this study was thus to compare the functionality of ASCs derived from the common technique of suction-assisted lipoaspiration (SAL) versus resection.MethodsHuman adipose tissue was obtained from paired abdominoplasty and SAL samples from three female donors, and was processed to isolate the stromal vascular fraction. Fluorescence-activated cell sorting was used to determine ASC yield, and cell viability was assayed. Adipogenic and osteogenic differentiation capacity were assessed in vitro using phenotypic staining and quantification of gene expression. Finally, ASCs were applied in an in vivo model of tissue repair to evaluate their regenerative potential.ResultsSAL specimens provided significantly fewer ASCs when compared to excised fat tissue, however, with equivalent viability. SAL-derived ASCs demonstrated greater expression of the adipogenic markers FABP-4 and LPL, although this did not result in a difference in adipogenic differentiation. There were no differences detected in osteogenic differentiation capacity as measured by alkaline phosphatase, mineralization or osteogenic gene expression. Both SAL- and resection-derived ASCs enhanced significantly cutaneous healing and vascularization in vivo, with no significant difference between the two groups.ConclusionSAL provides viable ASCs with full capacity for multi-lineage differentiation and tissue regeneration, and is an effective method of obtaining ASCs for cell-based therapies.

Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

“…Evidence is now emerging that harvesting methods may affect the quality and regenerative potential of ASCs in aspirated adipose tissue [3]. In previous reports we have established that SAL lipoaspirates are a functional ASC source when compared to excisional fat and that VASER UAL derived ASCs are not diminished in their regenerative capacities when compared to SAL ASCs [11, 12]. However, it is yet unclear if the two most popular UAL devices differ in their ability to preserve the stromal cellular components of the aspirated tissue and therefore in the resultant regenerative potential.…”

Fat Grafting with Tissue Liquefaction Technology as an Adjunct to Breast Reconstruction

“…Compared to en bloc resected fat, adipocytes harvested with SAL possess proportionately fewer adipose-derived stem cells, but those harvested without SAL possess similar viability and differentiation characterization [12]. This detailed analysis by Duscher et al verifies that SAL is an effective method of fat harvest for cell-based therapies [12]. Ultrasound-assisted suction lipectomy (UAL) initiates the rapid rarefaction and compression of tumescence fluid bubbles—lodged between adipocytes—to resonant size.…”

Vergleich des Regenerativen Zytokinprofils von Adipose Derived Stromal Cells (ASCs) Gewonnen Mittels Abdominoplastik, Suction Assisted Liposuction (SAL) und Ultrasound Assisted Liposuction (UAL)

“…Die regenerativen Potentiale von SAL und UAL ASCs wurden in aktuellen Studien mit ASCs aus en bloc resezierten Abdominoplastikpräparaten als vergleichbar beschrieben [37,40]. In Übereinstimmung mit diesen Daten konnte in unserer Evaluierung ebenfalls kein negativer Einfluss durch UAL und SAL auf Genexpressionsprofile von ASCs dargestellt werden (▶Abb.…”