Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells

Abstract

Regenerative medicine employs human mesenchymal stromal cells (MSCs) for their multi-lineage plasticity and their pro-regenerative cytokine secretome. Adipose derived mesenchymal stromal cells (ASCs) are concentrated in fat tissue and the ease of harvest via liposuction makes them a particularly interesting cell source. However, there are various liposuction methods and only few have been assessed regarding their impact on ASC functionality. Here we study the impact of the two most popular ultrasound-assisted liposuction (UAL) devices currently in clinical use VASER (Solta Medical; Hayward, CA, USA) and Lysonix 3000 (Mentor, Santa Barbara, CA, USA) on ASCs yield, viability, osteogenic and adipogenic differentiation capacity and in vivo regenerative performance. After lipoaspirate harvest and processing we sorted for ASCs using Fluorescent Assisted Cell Sorting (FACS) based on an established surface marker profile (CD34+CD31−CD45−). Both UAL samples demonstrated equivalent ASC yield and viability. VASER UAL ACSs showed higher osteogenic and adipogenic marker expression, however still a comparable differentiation capacity was observed. Soft tissue healing and neovascularization were significantly enhanced via both UAL derived ASCs in vivo and there was no significant difference between the cell therapy groups. Taken together, our data suggests that UAL allows safe and efficient harvesting of the mesenchymal stromal cellular fraction of adipose tissue and that cells harvested via this approach are suitable for cell therapy and tissue engineering applications.

Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing

“…To drive clinical translation, we confirmed these findings in a well-established murine diabetic wound model [7, 8, 12, 13, 25, 26, 41–43] in combination with an IVIS system and luciferase-marked ASCs, allowing us to track in vivo cell viability over time. While the harsh wound environment with diminished blood and nutritional supply rapidly depleted the injected ASCs, the fibrin niche prolonged ASC survival.…”

“…Although ASCs represent a promising wound-healing therapy, their clinical use is still limited due to variability in efficacy. As described in some of our previous studies, slight differences in harvesting and processing have great influence on differentiation, viability, and cytokine secretion of ASCs [7, 25–27]. Moreover, patient-specific factors contribute to the unpredictability of clinical success.…”

Preclinical evaluation for removal of bulging lower eyelid fat using ultrasound‐assisted lipolysis on a Yorkshire pig

“…Reports also suggest that UAL is a successful method for obtaining fully functional ADSCs that allows for the safe and efficient harvesting of mesenchymal stromal cells from the adipose tissue. Cells harvested via this approach are suitable for regenerative medicine purposes and autologous fat transfer 23‐25 …”

Use of Ultrasound-Assisted Liposuction for the Treatment of Breast Hypertrophy, Surgical Clinical Prospective Study on 45 Cases

“…The force of tension of the material determines which is destroyed and which is not. (Tension gradient) UAL allows safe and efficient harvesting of the mesenchymal stromal cellular fraction of adipose tissue and that cells harvested via this method are suitable for cell therapy and tissue engineering applications [14].…”