Successful Methods of Pancreas Transplantation in the Rat Using a Cuff Technique

Abstract

Two models of pancreas transplantation were examined in the rat with a view to choosing one for regular use in functional experiments. A cuff technique applied to the renal vessels of the recipient was used. The histology and endocrine functioning of whole pancreas-duodenal transplant (Tx) plus bladder drainage (drainage model), and the pancreatic duct ligated segmental pancreas Tx (ligated model) were examined. Syngeneic operations were performed using either Lewis or Wistar rats. Streptozotocin (STZ) 60mg/kg intravenously (i.v.) was used to render the recipient rats diabetic. A cuff technique was used with both models to anastomose the grafts to the renal vessels instead of the conventional technique of hand suturing to the abdominal vessels. This allows a shorter warm ischaemia time for the donor pancreas and leaves the systemic circulation intact leading to a high success rate for both techniques. Operation survival rates were 93% (n = 30) and 90% (n= 10) in the ligated and drainage models, respectively. The recipients in both groups were normoglycaemic for > 100 days. Histological examination revealed atrophic exocrine tissue early in the ligated group but only two from the drainage group showed exocrine atrophy at > 100 days. There was no statistical difference in i.v. glucose tolerance tests with both models showing a normal pattern. Thus, endocrine function remained independent of exocrine function. Both models are quicker than the conventional techniques. The duct ligation model was a simpler transplant to perform, suggesting that this should be the technique of choice in future experiments.

Model of mouse pancreaticoduodenal transplantation

“…Rat pancreas transplantation models with cuff techniques have been described previously, [12][13][14] with claimed advantages such as the elimination of clamping the systemic circulation, decreased ischemic time, and no bleeding after revascularization. In this study, we found mouse pancreas transplantation with cuff techniques to be extremely difficult.…”

Pancreas transplantation

“…[1] Di¤er bir uygulamada da ekzokrin sekresyonun ba¤ırsak içerisine veya mesane içerisine yapılmasıdır. [8] Bu nakillerde ilk uygulamlarda greft dokusunda görülen pankreatit ve idrar kaça¤ı sorunu gelitirilen tekniklerle, doku reddi (rejeksiyonu) de immünosüpesif tedaviyle önlenmitir (ekil 1). komplikasyonlar Sıklıkla komplikasyonlar ameliyatı takip eder, tromboz, pankreatit, enfeksiyon, kanama ve doku reddi eklindedir.…”

Model of mouse pancreaticoduodenal transplantation

“…Rat pancreas transplantation models with cuff techniques have been described previously, [12][13][14] with claimed advantages such as the elimination of clamping the systemic circulation, decreased ischemic time, and no bleeding after revascularization. In this study, we found mouse pancreas Figure 4.…”