Model of mouse pancreaticoduodenal transplantation

Tori, Masayuki; Ito, Toshinori; Matsuda, Hikaru; Shirakura, R; Nozawa, Masumi

doi:10.1002/(sici)1098-2752(1999)19:2<61::aid-micr4>3.0.co;2-e

Cited by 9 publications

(1 citation statement)

References 13 publications

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“…Despite major progress utilizing in vitro models, the development of animal models is crucial to deepen the knowledge of molecular mechanisms involved in IRI-associated graft alterations following pancreas transplantation. Several pancreas transplantation models have been developed in rodents 5 6 , but only one is reported in mice 7 . The Achilles heel of this highly demanding microsurgical procedure is the low survival rate of 46%.…”

Section: Introductionmentioning

confidence: 99%

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Cardini

Oberhuber

Hein

et al. 2017

JoVE

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Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the availability of the widest range of molecular probes and reagents to perform in vivo as well as in vitro studies. Based on our experience with various murine transplantation models, we developed a heterotopic pancreas transplantation model in mice with the intent to analyze mechanisms underlying severe ischemia reperfusion injury-associated early graft damage. In contrast to previously described techniques using suture techniques, herein we describe a new procedure using a non-suture cuff technique. In recent years, we have performed more than 300 pancreas transplantations in mice with an overall success rate of >90%, a success rate never described before in mouse pancreas transplantation. The backbone of this non-suture cuff technique for graft revascularization consists of two major steps: (I) pulling the recipient vessel over a polyethylene/polyamide cuff and fixing it with a circumferential ligature, and (II) placing the donor vessel over the everted recipient vessel and fixing it with a second circumferential ligature. The resultant continuity of the endothelial layer results in less thrombogenic lesions with high patency rates and, finally, high success rates.In this model, arterial anastomosis is achieved by pulling the abdominal aorta of the donor graft over the everted common carotid artery of the recipient animal. Venous drainage of the graft is achieved by pulling the portal vein of the graft over the everted external jugular vein of the recipient. This manuscript provides details and crucial steps of the organ recovery and organ implantation procedures, which will allow researchers with microsurgical skills to perform the transplantation successfully in their laboratories.

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Section: Introductionmentioning

confidence: 99%

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Cardini

Oberhuber

Hein

et al. 2017

JoVE

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Chimerism and bone marrow based therapies in transplantation

Siemionow

Nasır

2007

Microsurgery

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Composite tissue allotransplantation holds a great potential for providing increased knowledge of anatomy and microsurgical experience for life-enhancing reconstructions. Many transplant cases around the world have made this a clinical reality at the present time. Composite tissue allotransplants contain multiple tissue types, including bone, muscle, vessels, nerves, skin, and immune cells and bear a huge antigenic load. Although immunosuppressive drugs are applied successfully to prevent allograft rejection, their side effects pose a barrier to worldwide use. Bone marrow therapy in many tolerance induction protocols, therefore, provides a guide to reaching the target of permanent immunotolerance. Multiple studies suggest that bone marrow is immunomodulatory and may facilitate allograft acceptance. In this review, bone marrow based therapy protocols of clinical and experimental models are presented in two major categories: solid organ and composite tissue transplantation.

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