Subcutaneous cysticercosis diagnosed by mitochondrial DNA analysis

Shih, Yi Chin; Chen, Chien‐Ming; Huang, Yu Huei; Liao, Chien Wei; Fan, Chia Kwung; Yang, Chao-Ping

doi:10.1111/j.1365-4632.2009.04366.x

Cited by 5 publications

(4 citation statements)

References 10 publications

(40 reference statements)

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“…Primers targeting large subunit rRNA gene were selected to identify and amplify degraded DNA in degenerated and calcified cysts/lesions as Dalmasso et al (2004) have shown that degraded DNA can still be amplified by PCR if primers are designed to amplify a shorter target sequence, between 100 and 300 bp. Human neurocysticercosis from brain biopsy tissues (Harrington et al 2009), subcutaneous cysticercosis from histopathological specimens (Shih et al 2010) and bovine cysticercosis in postmortem inspection samples (Chiesa et al 2010) were confirmed by targeting cytochrome c oxidase subunit 1 (Cox1) gene. Lino Junior (2004) also reported the importance of PCR test with TBR primers as a reliable method for detection of cysticerci in tissues from human autopsies that are in advanced evolutive stages.…”

Section: Discussionmentioning

confidence: 99%

“…genomic DNA (gDNA) in dubious cysts, suspected lesions or cyst residues from pig and cattle in the slaughterhouse situation (Gonzalez et al 2006;Geysen et al 2007;Chiesa et al 2010). Polymerase chain reaction (PCR) test was also used in the diagnosis of cysticerci of T. solium from tissues of humans (Harrington et al 2009;Shih et al 2010). Therefore, in the present study PCR test was employed for the specific detection of T. solium cysts in suspected lesions and also for validation of meat inspection results, so that it could be developed as an appropriate postmortem test for validation of traditional meat inspection results.…”

Section: Introductionmentioning

confidence: 99%

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PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi

Hafeez

Kumar

et al. 2011

Trop Anim Health Prod

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Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi

Hafeez

Kumar

et al. 2011

Trop Anim Health Prod

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“…Neurocysti-cercosis (Space-occupying, cystic intracranial lesions, epilepsy) PCR [138,139] Nested PCR [140] Semi-nested PCR [141] PCR amplified DNA sequences targeting T. solium mitochondrial cox1 gene and cob gene [142] Loop-mediated isothermal amplification [143] II B [147]. Histoplasma capsulatum antigen and antibodies can be determined by different methods in CSF and are used to establish diagnosis.…”

Section: Cysticercus Cellulosaementioning

confidence: 99%

“…Table lists the CNS helminthoses, their neurological manifestation, and the modern molecular‐based diagnostic techniques. It needs to be stressed even more that in helminthic diseases, the direct visualization or detection of the helminths, either the adult worm, the larval stage, or the eggs, be it in body fluids or biopsied material, still represents the golden standard of diagnosis.…”

Section: Parasitesmentioning

confidence: 99%

EFNS‐ENS guidelines for the use of PCR technology for the diagnosis of infections of the nervous system

Steiner

Schmutzhard

Sellner

et al. 2012

Euro J of Neurology

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Background: Polymerase chain reaction (PCR) as a means to amplify nucleic acids has become an essential element in diagnosis of infections. It has evolved into a simple and rapid, easy-to-use approach. At present there are no published guidelines for the usage of PCR technology for the diagnosis of infections of the nervous system. Methods: We reviewed the advantages and pitfalls of PCR in order to guide neurologists and infectious diseases experts in its application for the diagnosis of infections of the nervous system. Medical reference systems were searched, and original papers, meta-analyses, review papers, book chapters and guidelines recommendations were reviewed. The final literature search was performed in May 2012. Recommendations were reached by consensus. Recommendations: The reliability of PCR technology for the diagnosis of neurological infections is currently based on the pathogens. The main contribution of PCR is to the diagnosis of viral infections followed by bacterial CNS infections with the notable exception of tuberculous meningitis. Efficacy for the diagnosis of protozoal infections and helminthic infestations has also been established in many instances. Unfortunately, current molecular PCR technology is far from becoming routine in resource-poor countries where such infections are prevalent. Despite the importance of fungal infections in the context of the immune-compromised host, there is not enough data to recommend the routine use of PCR. Conclusions: PCR technology is currently a reliable method for the diagnosis of viral and bacterial (except tuberculosis) infections, and only for some protozoal infections and helminthic infestations.

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