PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi, C.; Hafeez, Maryam; Kumar, Pradeep; Rayulu, V. C.; Subramanyam, K.V.; Sudhakar, K.

doi:10.1007/s11250-011-9893-2

Cited by 27 publications

(23 citation statements)

References 16 publications

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“…The PCR conditions were optimised using different temperature setups for annealing and concentrations of No. 05102015-00063-EN 5/16 reagents/chemicals as per previously described methods (22,23…”

Section: Polymerase Chain Reaction Amplificationmentioning

confidence: 99%

“…Some studies have reported similar detection limits (23) whereas others have reported lower detection limits (1 pg) for TBR primers(19). Higher detection limits (1 ng) for JB primers have also been reported(23). The variation in the detection limits of PCR using TBR and JB primers may be attributed to differences in sample preparation and DNA extraction methods.…”

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confidence: 97%

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Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India

Chawhan¹,

Sharma²,

Gill³

2015

Rev. Sci. Tech. OIE

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Porcine cysticercosis is a serious zoonosis in resource poor countries.Despite the evidence showing that the disease is endemic in the Punjab region of India, molecular characterisation of Taenia solium cysticercosis from naturally infected pigs has not been carried out.The authors examined a total of 519 pigs slaughtered in small slaughter shops (shops that sell meat from animals that are slaughtered on the premises as the customer waits) in the urban slums of Punjab state in northern India. The expected polymerase chain reaction (PCR) products with molecular sizes of 286 bp, 420 bp, 1150 bp and 333 bp corresponding to the targeted large subunit rRNA, cytochrome oxidase 1, internal transcribed spacer 1, and diagnostic antigen Ts14 genes, respectively, were amplified from the cysts collected from all 22 infected carcasses. The detection limits for the respective primers (except those targeting the Ts14 gene) were estimated. The analytical sensitivities of both the TBR and JB primers (targeting the rRNA and cytochrome oxidase genes, respectively) were found to be higher (10 pg) than that of the internal transcribed spacer 1 gene (1 ng) primers. Ten representative samples from cytochrome oxidase 1 gene amplified products were sequenced in both directions for phylogenetic analysis. Sequencing demonstrated that all cysticerci were of the Rev. Sci. Tech. Off. Int. Epiz., 34 (3) 2 No. 05102015-00063-EN 2/16 Asian genotype of T. solium and not of the African/Latin American genotype or T. asiatica. The results confirm the presence of T. solium porcine cysticercosis in Punjab state and there is therefore an urgent need for science-based policies for prevention and control of this serious zoonosis.

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Section: Polymerase Chain Reaction Amplificationmentioning

confidence: 99%

mentioning

confidence: 97%

Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India

Chawhan¹,

Sharma²,

Gill³

2015

Rev. Sci. Tech. OIE

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“…The PCR III method yielded products only when annealing temperature was lowered by 2 C (from 59 C to 57 C) with reference to original procedure described by Sredeevi et al (16). In such conditions, there were no nonspecific reactions with three other Taenidae parasites.…”

Section: Discussionmentioning

confidence: 99%

“…The method was based on the procedure described by Sreedevi et al (16), which was modified during investigation. Target sequence in this method encodes the T. solium cytochrome c oxidases ubunit 1 gene -this sequence was previously published by Yamasaki et al (18).…”

Section: Methodsmentioning

confidence: 99%

Optimisation and comparison of three PCR procedures for molecular identification of Taenia solium

Karamon

Sroka

Cencek

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to optimise selected PCR methods for identification of T. solium, and to compare their effectiveness and usefulness. The investigation concerned three PCR methods described earlier: PCR I (specific to oncospherespecific protein Tso31 gene), PCR II (specific to large subunit rRNA gene), and PCR III (cytochrome c oxidases ubunit 1 gene). Each of them needed optimisation in connection with some changes in the procedures. Among the examined procedures, PCR I was found to be the most useful, requiring the least corrections during optimisation -only a higher concentration of polymerase was necessary. Testing an optimised PCR II method showed strong unspecific reactions with E. granulosus and T. saginata. This method was not considered diagnostically useful in distinguishing T. solium. PCR III method yielded products only when annealing temperature was lowered by 2 C. Under such conditions, there were no unspecific reactions with three others Taenidae parasites; however, annealing at a temperature only 1 o C lower generated a distinct unspecific PCR product from T. saginata DNA. Therefore, this method was of limited usefulness. Comparison of the effectiveness of the two selected methods (PCR I and III) in detection of T. solium in successive DNA dilutions showed a large difference between them: in the same DNA sample, PCR I showed positive results in a sample diluted 1:3200, while PCR III failed at dilutions greater than 1:50. The results showed that among the three different methods used in the investigations, the most specific and effective for identification of T. solium was PCR I.

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“…Primarily, PCR tests have been applied to identify doubtful T. solium cystic lesions in pork meat and also to confirm the results obtained during inspection of meat from slaughtered pigs (41). Karamon et al (27) optimised three different PCR assays developed previously for identification of T. solium (32,41,47). Comparison of the effectiveness of these PCRs revealed that the most sensitive and specific one is nested PCR by Mayta et al (32).…”

Section: Diagnostics Of Porcine Cysticercosismentioning

confidence: 99%

Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite

Samorek-Pieróg

Karamon

Cencek

2018

Journal of Veterinary Research

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Taenia solium is a parasite causing porcine cysticercosis and human taeniosis and cysticercosis, parasitic zoonoses with a serious public health and economic influence. It has been globally ranked as the top foodborne parasite by the Food and Agriculture Organisation of the United Nations (FAO) and the World Health Organisation (WHO). This parasite is transmitted mainly in countryside regions where animals are free roaming, having access to human faeces, and infected pork is widely available. More developed countries eliminated cysticercosis; nonetheless, there are insufficient data about the current endemicity status of T. solium, due to increased human migration from endemic areas. Formally submitted statistics on cysticercosis in pigs are extremely inadequate. This is the result of not reporting all cases of the disease by some countries and lack of molecular verification during identification of the parasite. There is a need to develop diagnostic tests with increased sensitivity and specificity. The purpose of the present review is to summarise current knowledge about diagnostic and control methods concerning T. solium infection. The article does not address the diagnostics of human cysticercosis, since there is a distinct medical field which should be discussed separately. The paper focuses mainly on identifying the sources of T. solium infection, presenting the methods to detect and control porcine cysticercosis and taeniosis in humans.

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PCR test for detecting Taenia solium cysticercosis in pig carcasses

Cited by 27 publications

References 16 publications

Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India

Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India

Optimisation and comparison of three PCR procedures for molecular identification of Taenia solium

Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite

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