Sub-chronic toxicity study of ethanol stem-bark extract of Blighia sapida (Sapindaceae) in wistar rats

Abstract

Blighia sapida has been used in the treatment of different pathologies. The study aimed at evaluating the acute and sub-chronic toxicity of ethanol stem-bark extract of B. sapida. The acute toxicity was evaluated by gavage administration at single dose and the extract was also administered at doses of 250, 500 and 750 mg/kg body weight every other day for ninety day. No mortality or observable signs of toxicity were observed for acute and sub-chronic effects of the extract on the tested animals. No significant difference (P > 0.05) in haematological and biochemical parameters compared to the control group. However, histopathological observation revealed some derangements which could be due to continuous consumption of the extract by the animals. It implied that care must be exercised in the use of the plant for a long period of time to prevent its possible long-term toxic effects.

Effects of repeated administration of butanolic fraction of Blighia unijugata Bak. (Sapindaceae) leaves on some haematological parameters in wistar rats

“…This results in the release of hemoglobin [22] . These results are almost similar to those of [23] who showed that the ethanolic extract of the stem bark of Blighia sapida at 250, 500 and 750 mg/kg bw did not cause any significant variation in erythrocyte parameters. Likewise, the results of this study partly agree with [24] .…”

“…The lack of a significant effect on the relative organ weight suggests that this fraction had no toxic effect on the organs studied in rats during 28 days of experimentation. These results are comparable to those of [24] and [23] who showed respectively that the ethyl acetate extract of Lophira lanceolata leaves at 250, 500 and 1000 mg/kg bw and the ethanolic extract of the stem bark of Blighia sapida at 250, 500 and 750 mg/kg bw did not cause any significant change in the body weight of the rats. In addition, macroscopic observation of the internal organs of the rats did not reveal any abnormalities.…”

Antidiabetic activity and acute toxicity of combined extract of Andrographis paniculata, Syzygium cumini, and Caesalpinia sappan

“…This shows that the content in the ASCE extracts is safe for treating disease. An increase in the bodyweight of experimental animals during the study is a sign of animal welfare, conversely, a decrease in the body weight can be a sign of toxicity ( Adekola et al., 2020 ).…”

Promising Natural Products in Crop Protection and Food Preservation: Basis, Advances, and Future Prospects

“…Most crude extracts obtained from plants contain a mixture of compounds, which are used directly without any further purification. us, the active compounds are not separated from the inactive/toxic ones, which are responsible for crude extract toxicity [271,272]. Hence, isolation of the active compounds which exert the antimicrobial/antioxidant activity helps to eradicate the toxic compounds, which are not needed in such therapeutic mixtures.…”