Study the analgesic effect of diclofenac and silymarin coadministration in chicks

Albadrany, Yasser M.; Naser, Ahmed; Hasan, Mohammad Mahmudul

doi:10.33899/ijvs.2021.127065.1453

Cited by 5 publications

(5 citation statements)

References 19 publications

(21 reference statements)

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“…The chick model is one of the essential experimental models in the life sciences; it has been used in analgesia (18), neurobehavioral experiments (19), and toxicological studies. Diclofenac sodium-induced increased ALT and AST activity in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

The protective impacts of lipoic acid on diclofenac-induced hepatorenal toxicity in broiler chicks

Abdul-Ghani¹,

Naser²

2022

IJVS

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Alpha-lipoic acid is an anti-inflammatory and antioxidant compound that shows free radical scavenging actions and potent antioxidant properties on the metabolites of other cellular oxidants. The investigation of the defensive activity of alpha-lipoic acid (ALA) counter to diclofenac triggered liver and kidney damage in broiler chicks was the goal of this research. Chicks (7 days old) were distributed into four groups of six chicks each. The first group was the control received propylene glycol, the second group was injected intraperitoneally with Alpha-lipoic acid 80 mg/kg, the third group was injected intraperitoneally with diclofenac sodium at a dose of 2 mg/kg, and the fourth group was treated with ALA at 80 mg/kg and diclofenac at 2 mg/kg together. The trial continued for seven days. One day after the latest treatment, all the chicks were sacrificed by cutting jugular veins; blood samples were taken for biochemical analysis. Diclofenac causes a significant increase in ALT, AST, creatinine, and urea, while the coadministration of ALA with Diclofenac caused a significant decrease in ALT, AST, Creatinine, and Uric acid. Alpha-lipoic acid may benefit from counteracting diclofenac-induced hepatorenal toxicity due to antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

The protective impacts of lipoic acid on diclofenac-induced hepatorenal toxicity in broiler chicks

Abdul-Ghani¹,

Naser²

2022

IJVS

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“…Experiments were conducted when the ages of the chicks were between 7-9 days. This is a suitable experimental animal (chick) model used previously in pharmacological and toxicological studies (8)(9)(10)(11). The Alpha lipoic acid (Lipoic-Forte by AMS®), formalin, and their suppliers were as follows.…”

Section: Methodsmentioning

confidence: 99%

Assessment of the analgesic effect of Alpha-lipoic acid by three acute pain models

Rehab

Naser

2022

IJVS

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Alpha-lipoic acids are Known as a good analgesic in neuropathic pain, especially in diabetic patients. This research aimed to assess the analgesic activity of ALA by three acute pain models using broiler chicks. We used electrical stimulation, hot water test, and formalin test to elicit acute pain. The up and down method was used to calculate the median effect of the analgesic dose. The ED50 of ALA was 45.18 and 74.56 mg/kg intraperitoneally by electrical stimulation and hot water test, respectively. We demonstrated that the peak of analgesic effect was after one hour by using different doses and different times. ALA at 0, 75, 150, 300 mg/kg intraperitoneally produces a dose-dependent analgesic effect by formalin test. In conclusion: ALA induced analgesic activity, probably by closing voltage-gated calcium and or voltage-gated sodium channels. These outcomes show that therapeutic doses of ALA can affect pain and may mask or reduce nociception induced by acute pain models.

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“…On the tenth day of the experiment, groups 3 and 4 received MTX at 40 mg/kg (intraperitoneal injections) for five days (10,11). After 15 days of experimental, the animals in all groups were euthanized to collect tissue samples from liver and kidneys and blood for the biochemical test such as AST, ALT, AP, urea, creatinine, and uric acid concentration in blood serum (12,13).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of propolis on liver and kidney injury caused by methotrexate in chicks

Fadel¹,

Abdullah²,

Al-Mahmood³

et al. 2022

IJVS

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The current study aimed to explain propolis's protective effect on the liver and kidney damage caused by methotrexate (MTX). A total of 80 chickens at one day old were used and divided into three groups; the first group was the control group, the second group received the propolis, the second group was treated with MTX, and the fourth group received both propolis and treated with MTX. After 15 days of experimental, all chickens were euthanized, and blood samples and liver and kidney tissue were collected. The result showed that the treated group with MTX showed an increase in serum levels of AST, ALT, AP, urea, creatinine, and uric acid in comparison with both the control and propolis group, while in the group treated with both Propolis and MTX showed serum level of AST, ALT, AP, urea, creatinine, and uric acid similar to that recorded in both in the control group and MTX group. The liver sections treated with methotrexate showed hyperplasia of fibrocytes in the portal area with infiltration of mononuclear inflammatory cells represented by macrophages, and coagulative necrosis in affected hepatocytes, clear vacuoles in the hepatocytes, massive infiltration of macrophages. Sections of the liver treated with methotrexate and propolis explain a marked decrease in the fatty degeneration, with few infiltrations of mononuclear inflammatory cells around portal areas. The liver section from propolis treated group and the control group showed typical hepatic tissue architecture. The kidney sections treated with methotrexate showed coagulative necrosis in the endothelial cells, glomeruli appearing irregular in shape, and hemorrhaging in the extracellular matrix. The sections of the kidney treated with methotrexate and propolis explain a marked rise in the renal tubules with the typical feature of a healthy one. The section of the kidney from the propolis treated group and control group showed typical architecture of renal tissue. In conclusion, propolis greatly protects against MTX's toxic effect in chicks' liver and kidney.

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Study the analgesic effect of diclofenac and silymarin coadministration in chicks

Cited by 5 publications

References 19 publications

The protective impacts of lipoic acid on diclofenac-induced hepatorenal toxicity in broiler chicks

The protective impacts of lipoic acid on diclofenac-induced hepatorenal toxicity in broiler chicks

Assessment of the analgesic effect of Alpha-lipoic acid by three acute pain models

Protective effect of propolis on liver and kidney injury caused by methotrexate in chicks

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