Study on the behaviour of glucose oxidase immobilized on microfiltration polyamide membrane

Vasileva, N.; Godjevargova, Tzonka

doi:10.1016/j.memsci.2004.02.009

Cited by 23 publications

(12 citation statements)

References 12 publications

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“…Immobilization caused a drop in the values of both S* and H* of the enzyme ( Table 3), indicating that the conformation of the modified enzyme was altered in the direction of partially unfolded transition state or immobilized protein's hydrophobic core decreased with increase in thermo-stability. This study corroborated the work of other researchers who immobilized enzymes on micro-filtration polyamide membrane, glyoxyl agarose and epoxy sepa beads [31]. Alkyl amine controlled pore glass beads significantly enhanced thermostability.…”

Section: Thermal Stability and Thermodynamic Studiessupporting

confidence: 90%

A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Rajoka

Zia²,

Khalil-ur-Rehman³

2007

PPL

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Section: Thermal Stability and Thermodynamic Studiessupporting

confidence: 90%

A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Rajoka

Zia²,

Khalil-ur-Rehman³

2007

PPL

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“…The C1s spectrums were deconvolved into three component Gaussian peaks: peak (1) at 286.32 eV, attributed to the C-N group; peak (2) at 284.29 eV, considered as corresponding to C=O; and peak (3) at 282.44 eV, attributed to the C-H group. 21,43 Following ammonia-based plasma treatment, the C-N group at 286.32 eV was increased from 16.06% to 21.17%. 43 The N1s spectrums of untreated and ammonia-based plasma-modified woven PLA are shown in Figure 1(c).…”

Section: Resultsmentioning

confidence: 99%

Covalent immobilization of enzyme on aminated woven poly (lactic acid) via ammonia plasma: evaluation of the optimum immobilization conditions

Song

Yeo

et al. 2016

Textile Research Journal

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The present study aims to develop an immobilization support from woven poly (lactic acid) (PLA) and establish the optimum immobilization conditions for trypsin. Woven PLA was modified by ammonia-based plasma treatment in order to incorporate amine groups on its surface. X-ray photoelectron spectroscopy analysis showed that the N1s composition of PLA increased significantly, from 0.66% to 5.92%, after ammonia-based plasma processing. Trypsin from porcine pancreas was immobilized onto modified woven PLA by covalent binding after activating PLA with glutaraldehyde (GA). The results indicated that the optimal GA treatment conditions were as follows: pH of 10.0, 2% GA (v/v), and 180 min crosslinking time. In addition, the optimum immobilization conditions were as follows: pH of 8.5, 10% (owf) of trypsin concentration, 30 min, and 25℃. Under the optimum conditions, the amount of immobilized enzyme on woven PLA was 0.28 mg/mg and specific activity was 3.763 U/mg. In addition, the pH and thermal stabilities of the immobilized trypsin were improved. The immobilized trypsin retained approximately 55% of its initial activity after 20 days of storage and exhibited the potential for repetitive use through approximately 15 cycles. GA crosslinking and trypsin immobilization were found to improve the roughness of the PLA surface and increase its hydrophobicity. The data indicate that modified woven PLA, used as an immobilization support, shows suitable properties for use as a biocatalytic material in enzymatic applications.

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“…Figure 6 showed the increase in GOD concentration of the Si-PAAc-GOD surface, obtained at the GOD immobilization time of 5 h, with the increase in the PAAc layer thickness. Although steric hindrance from the thicker PAAc chains on the silicon surface increased, a number of GOD can be readily immobilized because of the high density of carboxyl groups on the Si-PAAc surface, compared to the amount of GOD immobilized on polymer films via adsorption (33), reversible immobilization (34), and amide linkage (35).…”

Section: Resultsmentioning

confidence: 99%

Poly(acrylic acid)-silicon Hybrids Prepared via a RAFT-mediated Process and Covalent Immobilization of Glucose Oxidase

Xia

et al. 2012

Journal of Macromolecular Science, Part A

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A surface modification technique was proposed for the modification of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with acrylic acid (AAc) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. With the aid of a water-soluble carbodiimide, GOD was then covalently immobilized on the silicon surface through the amide linkage between the amino group of GOD and the carboxyl group of the grafted AAc polymer. The changes in the surface composition after polymer grafting and enzyme immobilization on the silicon surface were investigated using X-ray photoelectron spectroscopy (XPS). The amount of GOD immobilized could be varied by changing the thickness of the polymer layer and the immobilization time. The GOD-functionalized silicon hybrids are potential useful in the application of the silicon-based biosensors.

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Study on the behaviour of glucose oxidase immobilized on microfiltration polyamide membrane

Cited by 23 publications

References 12 publications

A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

A Surface Immobilization Method of Endoglucanase from Cellulomonas biazotea Mutant Improved Catalytic Properties of Biocatalyst During Processing

Covalent immobilization of enzyme on aminated woven poly (lactic acid) via ammonia plasma: evaluation of the optimum immobilization conditions

Poly(acrylic acid)-silicon Hybrids Prepared via a RAFT-mediated Process and Covalent Immobilization of Glucose Oxidase

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