Poly(acrylic acid)-silicon Hybrids Prepared via a RAFT-mediated Process and Covalent Immobilization of Glucose Oxidase

Ji, Jiayou; Li, Lanyan; Xia, Keqiang; Li, Liang; Shang, Songmin

doi:10.1080/10601325.2012.662059

Cited by 8 publications

(4 citation statements)

References 33 publications

(32 reference statements)

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“…In the case of inorganic–organic (hybrid), “hairy” particles, the thickness of the polymer brush shell affects the amount of immobilized enzyme as well. Gopalan et al presented polymer brush-based model systems composed of poly(acrylic acid) or poly(2-vinyl-4,4-dimethyl azlactone) and demonstrated, that the surface density of RNase increased linearly as the thickness of the brushes increased. , The same tendency was found by Shang et al, who also confirmed that the amount of covalently immobilized glucose oxidase increased with the increase in thickness of the grafted poly(acrylic acid) . Further examples for this correlation were shown by the groups of Mao and Gao, who immobilized trypsin and lysozyme onto poly(glycidyl methacrylate)-based brushes.…”

Section: Introductionmentioning

confidence: 71%

“…20,21 The same tendency was found by Shang et al, who also confirmed that the amount of covalently immobilized glucose oxidase increased with the increase in thickness of the grafted poly(acrylic acid). 22 Further examples for this correlation were shown by the groups of Mao 23 and Gao, 24 who immobilized trypsin and lysozyme onto poly-(glycidyl methacrylate)-based brushes.…”

Section: Introductionmentioning

confidence: 96%

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Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Marschelke

Müller

Köpke

et al. 2018

ACS Appl. Mater. Interfaces

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Enzymes are described as ideal green biocatalysts because they are highly specific and selective. However, their practical application is hampered because of the low stability and missing reusability of free enzymes. One method to overcome these problems is the immobilization of enzymes onto carriers. Although numerous publications discuss different immobilization strategies, optimization of these carriers for the highest enzyme activity and loading capacity, enzyme selectivity, reusability, and reactor system configuration still remains a challenging task. In this contribution, we aim to address the role of the core−shell particle design with respect to their geometry as well as the polymer shell thickness on the immobilization of biomolecules. We discovered that spherical particles with a core diameter of 200 nm and intermediate shell thickness as well as platelet-like particles exhibited excellent results with a maximum immobilization yield of laccase from Trametes versicolor of up to 92% and an activity on the carrier material of 5.722 U/(g particle). Especially, the platelet-like particles offered a scalable and convenient alternative for the immobilization of laccase. Circular dichroism measurements proved that the secondary structure of the enzyme is not impaired by immobilization onto all kinds of carrier particles. Moreover, the immobilized laccase was successfully used for the decolorization of Cibacron blue P-3R in up to 18 cycles. Finally, particle separation was achieved via citrate-induced flocculation within 10 min. This detailed study contributes to the understanding of rational design of catalytically active hybrid materials and their effective performance at interfaces for applications in textile industry and environmental technologies.

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Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 96%

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Marschelke

Müller

Köpke

et al. 2018

ACS Appl. Mater. Interfaces

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“…Compared with conventional surfaces, a higher degree of biomolecule functionalization is achieved with P(M)AA brushes due to their 3D architecture and abundant carboxyl groups. Bovine serum albumin (BSA), , (strept)avidin, , biotin, antigen/antibody, ,, acetylcholinesterase, ribonuclease A (RNase A), glucose oxidase, folic acid, , fluorescent protein, arginine-glycine-aspartic acid (RGD) peptide, ,, collagen, , galactose, ,, and silk sericin were successfully immobilized on P(M)AA brushes to impart different functionalities for various purposes, including biosensing, ,,− catalysis, , targeting, , cell adhesion, and proliferation. ,,,,,,, …”

Section: Immobilization Of Biomoleculesmentioning

confidence: 99%

Synthesis and Biomedical Applications of Poly((meth)acrylic acid) Brushes

2015

ACS Appl. Mater. Interfaces

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Poly((meth)acrylic acid) (P(M)AA) brushes possess a number of distinctive properties that are particularly attractive for biomedical applications. This minireview summarizes recent advances in the synthesis and biomedical applications of P(M)AA brushes and brushes containing P(M)AA segments. First, we review different surface-initiated polymerization (SIP) methods, with a focus on recent progress in the surface-initiated controlled/living radical polymerization (SI-CLRP) techniques used to generate P(M)AA brushes with a tailored structure. Next, we discuss biomolecule immobilization methods for P(M)AA brushes, including physical adsorption, covalent binding, and affinity interactions. Finally, typical biomedical applications of P(M)AA brushes are reviewed, and their performance is discussed based on their unique properties. We conclude that P(M)AA brushes are promising biomaterials, and more potential biomedical applications are expected to emerge with the further development of synthetic techniques and increased understanding of their interactions with biological systems.

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“…In the past decade, the hybrid nanoparticles composed of an inorganic core and a polymer shell have attracted great interest due to the intriguing properties associated with the nanohybrids, such as outstanding optical, mechanical, rheological, electrical, catalytic, and fire retardance (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Polymerization Kinetics of Polystyrene Grafting using Azo-groups Bounded SiO₂as an Initiator

Liu

Zhang

et al. 2012

Journal of Macromolecular Science, Part A

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This work presents a new method for synthesis of inorganic/organic hybrid nanoparticles via the in-situ polymerization by the use of the azo-groups bounded silica nanoparticles as a radical initiator and styrene as a model vinyl-monomer. The synthesis and the structure of silica/polystyrene (SiO 2 /PS), and the polymerization kinetics of the styrene initiated by the azo-groups bounded SiO 2 nanoparticles are studied with techniques such as FTIR, XPS, DSC, GPC, and TEM. Results show that the SiO 2 -g-PS nanoparticles are synthesized successfully, and the resulting hybrid nanoparticles have a core-shell structure with SiO 2 in the core and the polystyrene on the outside layer. The percentage of the grafted PS on the SiO 2 surface increases with the progress of the polymerization before 6 h, and the largest amount of the grafted PS reaches 33% of the silica nanoparticles. Consequently, the size of the nanoparticles increases ca. 20 nm upon the polystyrene grafting. The molecular weight of the grafted PS increases with the polymerization, and it has reached a much large value in the first several polymerization hours while it keeps a constant value approximately in the following polymerization process. Meanwhile, the polydispersity index of the grafted PS gradually increases with the progress of the polymerization. These phenomena agree with the theory of the traditional free radical polymerization very well.

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Poly(acrylic acid)-silicon Hybrids Prepared via a RAFT-mediated Process and Covalent Immobilization of Glucose Oxidase

Cited by 8 publications

References 33 publications

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Synthesis and Biomedical Applications of Poly((meth)acrylic acid) Brushes

Synthesis and Polymerization Kinetics of Polystyrene Grafting using Azo-groups Bounded SiO₂as an Initiator

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Poly(acrylic acid)-silicon Hybrids Prepared via a RAFT-mediated Process and Covalent Immobilization of Glucose Oxidase

Cited by 8 publications

References 33 publications

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

Synthesis and Biomedical Applications of Poly((meth)acrylic acid) Brushes

Synthesis and Polymerization Kinetics of Polystyrene Grafting using Azo-groups Bounded SiO2as an Initiator

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Product

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Synthesis and Polymerization Kinetics of Polystyrene Grafting using Azo-groups Bounded SiO₂as an Initiator