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Aims:  To investigate the effect of molasses concentration, initial pH of molasses medium, and inoculum’s size to maximize ethanol and minimize methanol, fusel alcohols, acetic acid and aldehydes in the fermentation mash in industrial fermentors. Methods and Results:  Initial studies to optimize temperature, nitrogen source, phosphorous source, sulfur supplement and minerals were performed. The essential nutrients were urea (2 kg in 60 m3), 0·5 l each of commercial phosphoric acid and sulfuric acid (for pH control) added at the inoculum preparation stage only. Yields of ethanol, methanol, fusel alcohols, total acids and aldehydes per 100‐l fermentation broth were monitored. Molasses at 29°Brix (degree of dissolved sugars in water), initial pH 4·5, inoculum size 30% (v/v) and anaerobic fermentation supported maximum ethanol (7·8%) with YP/S = 238 l ethanol per tonne molasses (96·5% yield) (8·2% increase in yield), and had significantly lower values of byproducts than those in control experiments. Conclusions:  Optimization of process variables resulted in higher ethanol yield (8·2%) and reduced yield of methanol, fusel alcohols, acids and aldehydes. Significance and Impact of the Study:  More than 5% substrate is converted into byproducts. Eliminating or reducing their formation can increase ethanol yield by Saccharomyces cerevisiae, decrease the overall cost of fermentation process and improve the quality of ethanol.

The beginning of the Mesozoic, the Early Triassic, is characterized by several ecological perturbations following the end-Permian mass extinction. They are reflected in multiple C-isotope excursions coupled with climatic changes. Here we present palynological data from two accurately dated sections from the North Indian Margin (Pakistan and South Tibet). The climate of the Early Triassic was controlled by persistent monsoon circulation. The spore/pollen ratios, used as a proxy for humidity changes, indicate several significant climatic changes coinciding with C-isotope excursions. Comparison with published climate model simulations reveals that the climatic shifts were induced by orbital forcing and probably represent eccentricity cycles. Humidity peaks indicate an insolation forced shift of the intertropical convergence zone towards the North Indian Margin. Comparison with palynological data from Norway and other proxies reveal that the profound climatic change from humid to drier climate across the Smithian-Spathian boundary represents a global event, which affected southern and northern mid-latitudes and coincided with major ammonoid and conodont extinction events. This implies that increased greenhouse gas concentrations owing to recurring volcanic pulses increased the climate system sensitivity, resulting in climatic changes in distant parts of the world. Our data strongly support a link between C-isotope excursions, climatic changes and biotic responses.

In this research work, the antioxidant and metabolomic profiling of seven selected medicinally important herbs including Rauvolfia serpentina, Terminalia arjuna, Coriandrum sativum, Elettaria cardamom, Piper nigrum, Allium sativum, and Crataegus oxyacantha was performed. The in vivo cardioprotective potential of these medicinal plants was evaluated against surgically induced oxidative stress through left anterior descending coronary artery ligation (LADCA) in dogs. The antioxidant profiling of these plants was done through DPPH and DNA protection assay. The C. oxyacantha and T. arjuna showed maximum antioxidant potential, while the E. cardamom showed poor antioxidative strength even at its high concentration. Different concentrations of extracts of the said plants exhibited the protection of plasmid DNA against H2O2 damage as compared to the plasmid DNA merely treated with H2O2. The metabolomic profiling through LC-MS analysis of these antioxidants revealed the presence of active secondary metabolites responsible for their antioxidant potential. During in vivo analysis, blood samples of all treatment groups were drawn at different time intervals to analyze the cardiac and hemodynamic parameters. The results depicted that the group pretreated with HC4 significantly sustained the level of CK-MB, SGOT, and LDH as well as hemodynamic parameters near to normal. The histopathological examination also confirmed the cardioprotective potential of HC4. Thus, the HC4 being safe and inexpensive cardioprotective herbal combination could be considered as an alternate of synthetic drugs.

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