Study on Suitable Semen Additives Incorporation into the Extender Stored at Refrigerated Temperature

Abstract

The objective of this study was to compare the effect of Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX) and α-tocopherol (Vit E) on semen quality parameters of Karan Fries bulls. The fortification of extender by various semen additives improves motility as well as fertility of spermatozoa. Split samples of 24 ejaculates of four Karan Fries bulls were extended in extender with or without various additives such as BHT, PTX and Vit E, and performance was evaluated at an interval of 0, 24, 48 and 72 h at refrigerated temperature (4-7°C). Results of the present study revealed that addition of BHT, PTX and Vit E in extender improved sperm cell function, such as motility, viability, HOST, and acrosome integrity, as compared to the control during liquid storage up to 48 h of preservation at refrigerated temperature. There was no significant (p<0.05) difference between any of the additives up to 48 h of preservation. Overall, the results showed a significant (p<0.05) deterioration in motility after each storage interval. The results showed a significant deterioration in the acrosome integrity and plasma membrane integrity up to 48 h; subsequently, there was not much degradation of both the semen quality parameters. There was a significant increase in spermatozoal tail and total abnormality after each storage interval at refrigerator temperature (4 to 7°C); however, the head and mid-piece abnormalities were almost unaffected. Tail and total abnormality were least in extender fortified with BHT, PTX and Vit E at different hours of incubation as compared to the control. The addition of 1.5 mM BHT, 3.6 mM PTX and 1 mg/ml Vit E in the semen extender has more beneficial effect in terms of semen quality and preservability of spermatozoa.

Assessment of antioxidants for preservation of crossbred bull semen in Tris based extender

“…Moreover, freeze-thaw cycle reduces the level of antioxidant in mammalian semen (Stradaioli et al, 2007) and 2/3 rd decrease in glutathione level as well. Addition of antioxidant might be helpful in preventing the damage under such condition (Andrabi, 2009;Bhakat et al, 2011). It provide defense at three levels oxidation i.e., prevention, interception and repair (Sies, 1993).…”

Evaluación de diluyentes para la refrigeración de semen de conejo (Oryctolagus cuniculus)

“…Son pocas las publicaciones que reportan específicamente el uso de dextrosa en diluyentes para la refrigeración o la congelación de semen (Yulnawati et al, 2009;Bhakat et al, 2011). Así mismo, son escasos los reportes de la adición de acetato de potasio en los diluyentes, el cual se ha incluido principalmente en investigaciones con semen de cerdos y aves (Siudziñska y £ukaszewicz, 2008;Dziekoñska et al, 2009), no habiéndose encontrado investigaciones de ambos aditivos en semen de conejo.…”

Identification of Polymorphism of FSH Beta-Subunit Gene as Sperm Quality Marker in Bali Cattle Using PCR-RFLP

“…Methods of fresh semen collections and smears preparations was according to Arifiantini et al (2010) and Bhakat et al (2011), and were Dai et al (2009) performed from bull in AI centers. The samples were then delivered to the research Animal Genetic and Molecular Laboratory in Bogor Agricultural University.…”