Studies on the Male Antifertility Agent-Gossypol Acetic Acid. II. Effect of Gossypol Acetic Acid on the Motility and ATPase Activity of Human Spermatozoa

Kalla, N.R.; Vasudev, Monica

doi:10.1111/j.1439-0272.1981.tb00013.x

Cited by 58 publications

(11 citation statements)

References 6 publications

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“…ATP and ATPase play an important role in the signal transduction process coupled with the sperm motility (Mann and Mann, 1981). Any interference in production of ATP, and decline of ATPase activity inhibits the motility of spermatozoa (Kalla and Vasudev, 1981). It is suggested that on supplementation of FeAA to bull spermatozoa, ROS or oxygen free radicals are produced, which result in decrease in the intracellular ATP levels.…”

Section: Discussionmentioning

confidence: 99%

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Bansal

Bilaspuri

2008

Animal

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Numerous factors influence male fertility, one of these being the oxidative stress, which has elicited enormous interest recently. In sperm, induction of oxidation decreases motility and viability but increases lipid peroxidation (LPO). The optimum dose of ferrous ascorbate (FeAA: FeSO 4 1 ascorbic acid) for inducing oxidative stress by affecting motility, viability and LPO has been ascertained in local crossbred cattle bull spermatozoa. The fractions of spermatozoa suspended in 2.9% sodium citrate were subjected to three doses of FeAA (100 : 500, 150 : 750, 200 : 1000; mmol/l FeSO 4 : mmol/l ascorbic acid). These fractions were assessed for various parameters. Increase in the incubation period and promoter concentration induced a decrease in motility and viability, but an increase in LPO. Among three doses of FeAA, 150 : 750 mmol/l ascorbic acid is suggested to be the optimum/best dose as it induces the oxidative stress/LPO to a significant extent and also maintains better motility and viability as compared with the other two doses, and such conditions may enhance the fertilising potential of bull spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Bansal

Bilaspuri

2008

Animal

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“…4-Acetoxyphenol has been shown to be a potent activator of the NRF2-antioxidant response element pathway and is being tested as a possible treatment for neurodegenerative disease (20). Gossypol is a naturally occurring polyphenolic compound derived from cottonseed and was initially identified as an antifertility agent for males (21,22). Gossypol was identified as a BH3 mimetic and a potent inhibitor of Bcl-X L and, to a lesser extent, of Bcl-2 (23,24).…”

Section: Resultsmentioning

confidence: 99%

Gossypol Acetic Acid Prevents Oxidative Stress-Induced Retinal Pigment Epithelial Necrosis by Regulating the FoxO3/Sestrin2 Pathway

Hanus

Zhang

Chen

et al. 2015

Molecular and Cellular Biology

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The late stage of dry age-related macular degeneration (AMD), or geographic atrophy (GA), is characterized by extensive retinal pigment epithelial (RPE) cell death, and a cure is not available currently. We have recently demonstrated that RPE cells die from necrosis in response to oxidative stress, providing a potential novel mechanism for RPE death in AMD. In this study, we screened U.S. Food and Drug Administration-approved natural compounds and identified gossypol acetic acid (GAA) as a potent inhibitor of oxidative stress-induced RPE cell death. GAA induces antioxidative response and inhibits accumulation of excessive reactive oxygen species in cells, through which it prevents the activation of intrinsic necrotic pathway in response to oxidative stress. Sestrin2 (SESN2) is found to mediate GAA function in antioxidative response and RPE survival upon oxidative stress. Moreover, Forkhead box O3 transcription factor (FoxO3) is further found to be required for GAA-mediated SESN2 expression and RPE survival. Mechanistically, GAA promotes FoxO3 nuclear translocation and binding to the SESN2 enhancer, which in turn increases its transcriptional activity. Taken together, we have identified GAA as a potent inhibitor of oxidative stress-induced RPE necrosis by regulating the FoxO3/SESN2 pathway. This study may have significant implications in the therapeutics of age-related diseases, especially GA.A ge-related macular degeneration (AMD) is the leading cause of severe vision loss in people aged over 50, and its prevalence increases exponentially in people over the age of 70 (1). Currently, it is estimated that 1.75 million individuals suffer from this disease in the United States, and 7 million are said to be "at risk" (2). There are two types of AMD, the "dry" and "wet" forms, respectively. Dry AMD is a chronic disease that usually causes some degree of visual impairment and sometimes progresses to severe blindness. Dry AMD accounts for 90% of AMD cases and is currently without treatment available. The late stage of dry AMD, which is also knows as geographic atrophy (GA), is characterized by scattered or confluent areas of degeneration of retinal pigment epithelium (RPE) cells and the overlying photoreceptors that rely on the RPE for trophic support (3). AMD is a multifactorial disease with unclear etiology. Age is the most consistent risk factor associated with AMD, and genetic factors, oxidative stress, and inflammation also significantly contribute to AMD pathogenesis (4). Cigarette smoking, which induces systemic oxidative stress, has been proved to be a significant risk factor for AMD. Consistently, clinical studies have shown that the progression of AMD can be slowed with antioxidant vitamins and zinc supplements (5, 6). The retina is one of the highest oxygen-consuming tissues in the human body and, in particular, RPE is vulnerable to oxidative damage (7,8). The mechanism of RPE cell death in response to oxidative stress and in GA has been controversial. Apoptosis was suggested as a major mechanism of RPE cell ...

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“…It is possible that hydroethanolic extract of H. acida stem bark inhibited the uncoupling reaction of oxidative phosphorylation (Kalla and Vasudeva, 1981) and hence rendered the spermatozoa immotile.…”

Section: Discussionmentioning

confidence: 99%

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

Abu¹,

Uchendu²

2010

J. Med. Plants Res.

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Oral administration of aqueous ethanolic extract of Hymenocardia acida stem bark for eight weeks caused a significant reduction (P < 0.05) in the weights of testes, epididymides, ventral prostrate, seminal vesicles and vasa differentia compared to the control. The treatment related decreases in sperm motility, count, viability and serum testosterone returned to near normal levels on withdrawal of the extract. The groups that received doses of 100, 200 and 400 mg/kg body weight of the extract showed 40, 0 and 0% fertility, respectively. H. acida stem bark extract adversely affected spermatogenesis in Wistar rats.

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Studies on the Male Antifertility Agent-Gossypol Acetic Acid. II. Effect of Gossypol Acetic Acid on the Motility and ATPase Activity of Human Spermatozoa

Cited by 58 publications

References 6 publications

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Gossypol Acetic Acid Prevents Oxidative Stress-Induced Retinal Pigment Epithelial Necrosis by Regulating the FoxO3/Sestrin2 Pathway

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

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