Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Bansal, Ashish; Bilaspuri, G. S.

doi:10.1017/s1751731107000961

Cited by 38 publications

(40 citation statements)

References 13 publications

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“…Such system integrating ferrous and ascorbate ions reflects well on the chemistry and redox properties of iron, which as a transition metal has the ability to cause oxidative depletion of sperm lipids, proteins and DNA through the Fenton and Haber-Weiss reaction [6, 21, 24, 25]. …”

Section: Introductionmentioning

confidence: 99%

Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Tvrdá

Kováčik

Paál

et al. 2016

J Animal Sci Biotechnol

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BackgroundLycopene (LYC) is a natural carotenoid with powerful reactive oxygen species (ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate (FeAA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment (0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of FeAA (150 μmol/L FeSO4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, ROS generation was quantified via luminometry and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) as well as the concentrations of glutathione (GSH) and malondialdehyde (MDA).ResultsFeAA treatment led to a reduced spermatozoa motility (P < 0.001), viability (P < 0.001) and a decline of the antioxidant capacity of spermatozoa (P < 0.001) but increased the ROS generation (P < 0.001), superoxide production (P < 0.001) and lipid peroxidation (P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters (P < 0.001), mitochondrial activity (P < 0.001) and antioxidant characteristics (P < 0.001 with respect to SOD; P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.ConclusionsOur results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells.

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Section: Introductionmentioning

confidence: 99%

Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Tvrdá

Kováčik

Paál

et al. 2016

J Animal Sci Biotechnol

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“…One of the most widely used techniques to assess semen oxidative status is the measurement of lipid peroxidation by-products after incubation with an OS promoter system, thus evaluating sperm susceptibility to an oxidative environment (Aitken et al 1993, Bansal & Bilaspuri 2008, Simõ es et al 2013, Mojica-Villegas et al 2014. In rams, there is little data about the OS effect on sperm DNA packaging and fragmentation, especially under physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Hamilton¹,

Castro²,

Delgado³

et al. 2016

Reproduction

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Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm.

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“…They are capable of inducing DNA damage and decrease in fertility during storage of semen [38]. The peroxides are generally associated with decreased sperm functions and viability but also have a significant enhancing effect on the ability of spermatozoa to bind with homologous and heterologous zona pellucida [39].…”

Section: Lipid Peroxidation: Detrimental Effects On Sperm Functionsmentioning

confidence: 99%

“…2) [29]. Oxidative stress is also known to affect the integrity of sperm genome inducing high frequency of single and double DNA breaks [38]. The oxidative stress induced by white blood cells has a damaging effect on PUFAs of the sperm phospholipids resulting in decreased membrane fluidity [14].…”

Section: Oxidative Stress and Its Impacts On Semenmentioning

confidence: 99%

Antioxidants and Other Potent Strategies to Reduce Oxidative Stress in Semen

Bansal

2014

Free Radicals in Human Health and Disease

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Oxidative stress (OS) has been considered a major contributory factor to the male infertility. It is the result of imbalance between the reactive oxygen species (ROS) and antioxidants in the body which can lead to sperm damage, deformity, and eventually male infertility. Although high concentrations of the ROS cause sperm pathology (ATP depletion) leading to insufficient axonemal phosphorylation, lipid peroxidation, and loss of motility and viability, but many evidences demonstrate that low and controlled concentrations of these ROS play an important role in sperm physiological processes such as capacitation, acrosome reaction, and signaling processes to ensure fertilization. ROS are also generated during cryopreservation of spermatozoa for AI practices. To reduce the oxidative stress, there are certain compounds and reactions which dispose, scavenge, and suppress the formation of ROS or oppose their actions are called antioxidants. Currently, many antioxidants are under investigation. The supplementation of a cryopreservation extender with antioxidant has been shown to provide a cryoprotective effect on mammalian sperm quality. This chapter explains the impacts of oxidative stress and reactive oxygen species on spermatozoa functions, causes of ROS generation, and antioxidative strategies to reduce this OS. This study also suggests that antioxidant supplementation could be of clinical importance in prolonging the spermatozoal storage for assisted reproductive techniques (ARTs) like artificial insemination (AI), in vitro fertilization (IVF), and intrauterine insemination (IUI) purposes.

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Effect of ferrous sulphate and ascorbic acid on motility, viability and lipid peroxidation of crossbred cattle bull spermatozoa

Cited by 38 publications

References 13 publications

Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

Antioxidants and Other Potent Strategies to Reduce Oxidative Stress in Semen

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