Studies of tumour invasion in organ culture

Yarnell, Margaret M.; Ambrose, E. J.

doi:10.1016/0014-2964(69)90076-0

Cited by 27 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The appearance of the invasion in some of the control cultures was similar to the 9c en bloc " type described by Yarnell and Ambrose (1969a) whilst in other control cultures invasion was scanty. However, in those cultures challenged with crude rat liver histone, the cells invaded throughout the explant in some cases and considerably more than the controls in all cases (Fig.…”

Section: Cell Linessupporting

confidence: 72%

“…4) (Defendi, Lehman and Kraemer, 1963), partly because they are capable of forming multiple layers in culture. However, the in vitro invasion of normal and polyoma-transformed BHK21 cells has been compared and characterised by Yarnell and Ambrose (1969a) (Latner and Longstaff, 1971). …”

Section: Cell Linesmentioning

confidence: 99%

“…The presence of proteolytic enzymes in bistone preparations (Phillips and Johns, 1959) might be a contributing factor to the phenomenon. Increased invasion has been brought about by the incorporation of 0-I % trypsin into the culture medium of BHK2 I /embryonic heart cultures (Ysrnell and Ambrose, 1969b) (Ambrose, 1966). Histone could certainly be expected to affect cell charge, but the effect would be to decrease the net cell charge since histone is polyeationic.…”

Section: Cell Linesmentioning

confidence: 99%

“…Histone could certainly be expected to affect cell charge, but the effect would be to decrease the net cell charge since histone is polyeationic. Also, evidence against invasion resulting from changes in cell charge has recently been published by Yarnell and Ambrose (1969a The metabolism of normal and polyoma-transformed BHK21 cells has been studied by Paul, Broadfoot and Walker (1966). Glucose utilisation was found to be greater in the polyoma-transformed cells than in the normal BHK21 cells in both aerobic and anaerobic conditions.…”

Section: Cell Linesmentioning

confidence: 99%

“…A further characteristic of malignant cells is their ability to invade normal tissue in vitro (Yarnell and Ambrose, 1969a) and we report here the effect of histones on the invasiveness of four cell lines in culture.…”

mentioning

confidence: 95%

See 4 more Smart Citations

Invasive Properties of Histone Transformed Cells

Latner¹,

Longstaff²,

Lunn³

1971

Br J Cancer

View full text Add to dashboard Cite

Histone preparationChromatin was isolated from the livers of adult rats (Scott-Russ strain) according to the method of Bonner et al. (1968), omitting centrifugation through dense sucrose solution. Histone was extracted from the isolated chromatin by blending with 0-25N HCI. The insoluble material was centrifuged off and the extraction procedure repeated. The supernatants were pooled and histone was precipitated from the acid extract by adding 9 volumes of acetone. The precipitate was washed with acetone, air dried and finally vacuum dried. Tissue culture method8The technique adopted to study invasion in vitro involved the use of modified Trowell organ culture flasks. The apparatus (Fig. 1)

show abstract

Section: Cell Linessupporting

confidence: 72%

Section: Cell Linesmentioning

confidence: 99%

Section: Cell Linesmentioning

confidence: 99%

Section: Cell Linesmentioning

confidence: 99%

mentioning

confidence: 95%

See 3 more Smart Citations

Invasive Properties of Histone Transformed Cells

Latner¹,

Longstaff²,

Lunn³

1971

Br J Cancer

View full text Add to dashboard Cite

show abstract

Chemie und Biologie der Acylneuraminsäuren

Schauera

1973

Angew. Chem.

View full text Add to dashboard Cite

In Gangliosid-und in vielen Glykoproteinmolekiilen nehmen Acylneuraminsluren eine sowohl raumlich als auch biologisch-funktionell herausragende Stellung ein. Acylneuraminsauren sind N-und zum Teil auch 0-acylierte Derivate der Neuraminsaure, einer Polyhydroxyamino-ketosiiure, welche die Eigenschaften eines Kohlenhydrats besitzt und daher zur glykosidischen Bindung fahig ist. In diesem Fortschrittsbericht wird ein Uberblick uber unsere Kenntnisse von der Chemie und der Biologie der vor allem im Tierreich weit verbreiteten Acylneuraminsauren gegeben.

show abstract

Chemistry and Biology of the Acylneuraminic Acids

Schauer

1973

Angew. Chem. Int. Ed. Engl.

100

View full text Add to dashboard Cite

Acylneuraminic acids occupy an outstanding position both sterically and with respect to biological functions in gangliosides and in many glycoprotein molecules. Acylneuraminic acids are N-acylated and partly 0-acylated derivatives of neuraminic acid, a polyhydroxyamino keto acid that has the properties of a carbohydrate and is therefore capable of forming a glycoside linkage. The present report provides a survey of our knowledge of the chemistry and biology of the acylneuraminic acids, which occur widely in particular in the animal kingdom.

show abstract

Studies of tumour invasion in organ culture

Cited by 27 publications

References 13 publications

Invasive Properties of Histone Transformed Cells

Invasive Properties of Histone Transformed Cells

Chemie und Biologie der Acylneuraminsäuren

Chemistry and Biology of the Acylneuraminic Acids

Contact Info

Product

Resources

About