Invasive Properties of Histone Transformed Cells

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Neonatal hamster kidney cells (BHK21/C13), challenged in monolayer culture for three days with crude rat liver histone, have been shown to exhibit increased malignant characteristics when injected subcutaneously into hamsters. In contrast to the controls, the challenged cells produced tumours which invaded either the epidermis or the body wall of their hosts and frequently caused extensive visceral metastases. In vitro studies of the cell cultures, during and after histone treatment, suggested that cellular “transformation” rather than selection was effected by the crude histone preparation. Cells from the primary tumours of both control and test groups appeared morphologically identical but after sub-culture in vitro they retained their respective growth characteristics on reinoculation. Images p222-a Fig. 1 p224-a Fig. 2 Fig. 3

supporting

confidence: 53%

Enhanced Malignant Behaviour of Cells Treated with Crude Rat Liver Histone

Latner¹,

Longstaff²,

Turner³

1973

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“…The invasion of mouse (Bar Harbor strain 129) kidney cortex explants by polvoma virus transformed hamster kidney cells (BHK21/C13/Py, Macpherson and Stoker, 1962) was investigated using the mixed cell/ organ culture technique developed by Latner et al (1971). Monolayers of cells were grown in the filter wells of the apparatus and kidney explants cultured above them.…”

Section: Methodsmentioning

confidence: 99%

“…The bh oad spectrum protease inhibitor aprotinin (Trasylol, Bayer) was selected for study because it is known to accunmulate rapidly in the renal tissues when admiinistered intravenously (Haberland, 1967 (Latner et al, 1971).…”

Section: Methodsmentioning

confidence: 99%

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Inhibition of Malignant Cell Invasion in vitro by a Proteinase Inhibitor

Latner¹,

Longstaff²,

Pradhan³

1973

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Summary.-The inhibitory effect of the protease inhibitor aprotinin (Trasylol) on the invasion of mouse kidney explants by polyoma virus transformed BHK21 cells was investigated using a mixed cell/organ culture technique. The extent of invasion was monitored by following the changes in LDH isoenzyme pattern in the explants and by histological assessment. The kidney explants containing aprotinin were found to maintain a normal kidney LDH pattern and to suffer considerably less invasion than the explants not containing the drug. These results support the idea that proteolytic enzymes are associated with invasion and that inhibitors of protease activity could possibly be useful in the management of clinical cancer.

“…Various attempts to investigate the process in vitro have achieved some success (Easty and Easty, 1963;Barski and Wolff, 1965;Yarnell and Ambrose, 1969a, b;Latner, Longstaff and Lunn, 1971) but have not been susceptible to quantitative analysis. The methods used have frequently involved the confrontation of tumour cells with the cut edges of normal tissues.…”

mentioning

confidence: 99%

Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

Easty¹,

Easty²

1974

Organ cultures of chorioallantoic membranes of hen eggs have been used to establish a quantitative method of measuring the infiltrative ability of a variety of normal and tumour cells. Normal fibroblasts, mouse peritoneal cells and cells of low tumorigenicity infiltrated poorly and slowly whereas most tumours infiltrated rapidly. Some cells of the more invasive tumours achieved minimum rates of migration through the normal tissue of 2-3 μm/h. One tumour line which tended to form aggregates on the chorioallantoic membrane elicited a pronounced rejection response from the ectoderm of the membrane. Colcemid, which inhibits the formation of cell processes and the directional migration of cells, and dibutyryl cyclic AMP, which restores certain aspects of normal behaviour to tumour cells in vitro, had little effect on the invasion of the membrane by tumour cells. Images Fig. 9 Fig. 10 Fig. 11 Fig. 12 Fig. 5 Fig. 6 Fig. 7 Fig. 8