Structure of a Bacilliform Virus in Dendrobium as revealed by Negative Staining

Morphology and Intracellular Behaviour of Coffee Ringspot Virus (CRV) in Tissues of Coffee (Coffea arabica L.)

“…The first electron microscopic investigations on coffee ringspot showed the presence of rod-shaped, tubular structures, measuring 35-40 nm X 100-110 nm -similar to the inner components of rhabdovirus -located generally in the nucleus and occasionally in elements of the endoplasmic reticulum of cells of foliar tissues taken from naturally affected coffee plants (KITAJIMA and COSTA 1972). Similar particles were also found closely associated with Citrus leprosis (KITAJIMA et al 1972) and foliar spotting of ordiids (PETZOLD 1971, DuvEL and PETERS 1971, BEGTRUP 1972, LESEMANN and BEGTRUP 1971, KITAJIMA et al 1974, LESEMANN and DORAISWAMY 1975a, 1975b, Doi et al 1977) their viral etiology being suggested. In some instances in which besides these tubular, rod-like particles, also appeared larger, cytoplasmic membranesurrounded particles, the former were interpreted as mner components and the latter as the virions (LESEMANN and BEGTRUP 1972, LESEMANN and DORAIS-wAMY 1975a, 1975.…”

Bullet-shaped Virus-like Particles in Chlorotic and Necrotic Leaf Lesions of Orchids

“…Negatively stained crude sap preparations were made from tissues at the edge of leaf lesions. Similar to BEGTRUP (1972), we found that bullet-shaped particles (BSP) were readily detected in preparations negatively stained with ammonium molybdate (AM). Small pieces of leaf tissue were crushed in two drops of a 2 % solution of AM adjusted to pH 6.5 with NH.OH.…”

Rodlike Particles Associated with Ringspot Symptoms in Several Orchid Species in Brazil

“…Negatively stained leaf dip preparations either using phosphotungstic acid (KITAJIMA 1965) or ammonium molybdate (BEGTRUP 1972) were also made from most of these samples, and examined in the electron microscope.…”

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Rodshaped, viruslike particles have been reported in association with ringspot in ordiids {Dendrobium antennatum, D. inoglossum, Dendrobium hybrids and Phalaenopsis Lueddemanniana in some European countries (PET-ZOLD 1971, DiJVEL and PETERS 1971, LESEMANN and BEGTRUP 1972, BEGTRUP 1972. A recent survey in a large collection of orchids kept at the Dept.

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