Morphology and Intracellular Behaviour of Coffee Ringspot Virus (CRV) in Tissues of Coffee (Coffea arabica L.)

Chagas, C. M.

doi:10.1111/j.1439-0434.1980.tb03791.x

Cited by 14 publications

(7 citation statements)

References 12 publications

(5 reference statements)

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“…and T. Duran) Lebrun cv. Excelsa (Figure 1 D) Transmission electron microscopy detected the previously described cytopathic effects induced by CoRSV (Kitajima and Costa, 1972;Chagas, 1980) in the cells of the leaf and fruit lesions. Several nuclei of the parenchymal and epidermal cells contained an electron lucent inclusion in the nucleus, referred to as viroplasma.…”

Section: Resultssupporting

confidence: 54%

“…The disease has been observed in several regions of Brazil (Chagas et al, 2003;Kitajima and Chagas, 2009) and outside Brazil, confirmed only in Costa Rica (Rodrigues et al, 2002). The viral nature of the Coffee ringspot was inferred by electron microscopy which revealed the presence of short rod-like particles in the nucleus and cytoplasm and a characteristic electron lucent inclusion (viroplasma) in the nucleus (Kitajima and Costa, 1972;Chagas, 1980), its transmission by the tenuipalpid mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae) (Chagas, 1973), and mechanical transmission to Chenopodium quinoa Willd., C. amaranticolor Coste & Reyn. and Gomphrena globosa L. (Chagas et al, 1981).…”

Section: Introductionmentioning

confidence: 98%

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Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Kitajima¹,

Chagas²,

Braghini

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Coffee ringspot is a minor coffee disease caused by the nuclear type of Brevipalpus mite-transmitted virus, Coffee ringspot virus (CoRSV). Recently outbreaks of the disease in some growing regions of the state of Minas Gerais, Brazil, were registered with qualitative and quantitative yield losses. Coffea arabica was the only species registered as natural host. A survey was made on a germplasm collection of Coffea and related species kept at the Centro de Café "Alcides Carvalho", Instituto Agronômico, Campinas, state of São Paulo (SP), Brazil, to assess natural susceptibility of Coffee species, other than C. arabica and some interspecific hybrids of Coffea as well as other non-Coffea plant species to the Coffee ringspot virus (CoRSV). The following plants were found with ringspot symptoms on their leaves and/or fruits besides C. arabica L.: C. kapakata (IAC 4511), C. dewevrei cv. Excelsa, C. canephora cv. Robusta, hybrid derivative of the C. arabica × C. racemosa (IAC1195-5-6-2), C. arabica × C. dewerei (Piatã IAC 387), Híbrido de Timor CIFC 832/1 (derivative from a natural crossing between C. arabica × C. canephora) and C. racemosa. Also Psilanthus ebracteolatus, a species close to the genus Coffee was also found with ringspot lesions on their leaves. All these plants were also found infested by Brevipalpus mites identified as B. phoenicis. Infection of these plants by CoRSV was confirmed by the observation of characteristic cytopathic effects in the tissues of the lesion and by RT-PCR using a pair of primer specific for CoRSV. Only with C. racemosa RT-PCR failed to amplify the CoRSV genome. The susceptibility of P. ebracteolatus to CoRSV adds new dimension regarding its controversial taxonomic position

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Section: Resultssupporting

confidence: 54%

Section: Introductionmentioning

confidence: 98%

Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Kitajima¹,

Chagas²,

Braghini

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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“…In particle morphology and cytopathic effects, OFV closely resembles citrus leprosis Colariccio et al, 1995;Rodrigues et al, 2003). Coffee ringspot virus Chagas, 1980), Viola ringspot virus (Gowanlock et al, 1995), Clerodendron chlorotic spot virus (Kitajima and Moraes, 2000) and Hibiscus chlorotic spot virus, all of which are transmitted or associated with the mite Brevipapus phoenicis (Geijskes) (Kitajima et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Orchid Fleck Virus: Brevipalpus californicus Mite Transmission, Biological Properties and Genome Structure

2003

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Orchid fleck virus (OFV) causes necrotic or chlorotic ring spots and fleck symptoms in many orchid species world-wide. The virus has non-enveloped, bacilliform particles of about 40 nm x 100-150 nm and is sap-transmissible to several plant species. OFV is transmitted by the mite Brevipalpus californicus (Banks) in a persistent manner and efficiently transmitted by both adults and nymphs, but not by larvae. Viruliferous mites retain their infectivity for 3 weeks on a virus-immune host. The genome of OFV consists of two molecules of 6431 (RNA1) and 6001 nucleotides (RNA2). The RNAs have conserved and complementary terminal sequences. RNA1 contains five open reading frames (ORF), and RNA2 encodes a single ORF. Although some of the encoded proteins of OFV have sequences similar to those of proteins of plant rhabdoviruses, OFV differs from viruses in the family Rhabdoviridae in having a bipartite genome.

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“…CoRSV causes chlorotic spots on the leaves and ring spot on the bemes. The virus is composed of an inner component similar to CiLV but, by contrast with CiLV, full rhabdovirus particles exist (Chagas, 1980). Citrus zonate chlorosis is the only disease suspected to be associated to B. phoenicis (Rossetti et a[., 1965;Rossetti, 1975b;Rossetti, 1996) whose causal agent is unknown (Kitajima et af., 1972).…”

Section: Coffee Ringspot Clustermentioning

confidence: 98%

Citrus leprosis virus: properties, diagnosis, agro‐ecology and phytosanitary importance

Lovisolo¹

2001

EPPO Bulletin

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Citrus leprosis disease, caused by citrus leprosis virus (CiLV), had severe effects on sweet oranges in Florida (US) until the 1920s, after which it became rare. In South America, it appeared in the 1930s, first in Argentina and then in Brazil, where it is now widespread and very dangerous. It has also been reported in Paraguay, Peru, Uruguay and Venezuela. CiLV is transmitted by three species of Brevipalpus, mainly Brevipalpus phoenicis. The virus mainly attacks sweet orange, but also citrange, citron, Cleopatra mandarin, grapefruit, lemon, mandarin, sour orange and tangor. CiLV is a non‐enveloped rhabdovirus characterized by bacilliform particles measuring 120–130 × 50–55 nm, present in mesophyll and vascular parenchyma within cisternae of the endoplasm reticulum. Viroplasm structures are present in the infected cells. CiLV has been partially purified and its dsRNA as been investigated. It is mechanical transmissible to 13 test plant species belonging to the genera Atriplex, Beta and Chenopodium (Chenopodiaceae), Gomphrena (Amaranthaceae), and Tetragonia (Tetragoniaceae). Using some of these herbaceous test plants, grown at a suitable temperature, it is possible to diagnose CiLV in 3–4 days. CiLV is covered by lists and requirements in phytosanitary regulations, but the information given is often misleading. For phytosanitary purposes, it is important to consider the following main points: (a) both CiLV and its vectors need to be considered; (b) sweet orange fruits can be infected even more than propagation material; and (c) CiLV does not infect susceptible citrus systemically, or any of its known hosts.

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Morphology and Intracellular Behaviour of Coffee Ringspot Virus (CRV) in Tissues of Coffee (Coffea arabica L.)

Cited by 14 publications

References 12 publications

Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Orchid Fleck Virus: Brevipalpus californicus Mite Transmission, Biological Properties and Genome Structure

Citrus leprosis virus: properties, diagnosis, agro‐ecology and phytosanitary importance

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