Bullet-shaped Virus-like Particles in Chlorotic and Necrotic Leaf Lesions of Orchids

Morphology and Intracellular Behaviour of Coffee Ringspot Virus (CRV) in Tissues of Coffee (Coffea arabica L.)

“…The first electron microscopic investigations on coffee ringspot showed the presence of rod-shaped, tubular structures, measuring 35-40 nm X 100-110 nm -similar to the inner components of rhabdovirus -located generally in the nucleus and occasionally in elements of the endoplasmic reticulum of cells of foliar tissues taken from naturally affected coffee plants (KITAJIMA and COSTA 1972). Similar particles were also found closely associated with Citrus leprosis (KITAJIMA et al 1972) and foliar spotting of ordiids (PETZOLD 1971, DuvEL and PETERS 1971, BEGTRUP 1972, LESEMANN and BEGTRUP 1971, KITAJIMA et al 1974, LESEMANN and DORAISWAMY 1975a, 1975b, Doi et al 1977) their viral etiology being suggested. In some instances in which besides these tubular, rod-like particles, also appeared larger, cytoplasmic membranesurrounded particles, the former were interpreted as mner components and the latter as the virions (LESEMANN and BEGTRUP 1972, LESEMANN and DORAIS-wAMY 1975a, 1975.…”

Orchid fleck virus is a rhabdovirus with an unusual bipartite genome

“…In ultrathin sections of infected orchid leaf tissue, OFV or OFV-like agents induce a characteristic, intranuclear, electron-lucent viroplasms and a large number of virions have been found in nuclei (Chang et al, 1976;Kitajima et al, 1974;Lesemann & Doraiswamy, 1975). Virions could be seen scattered throughout the viroplasm, and they are often found associated perpendicularly with the inner membrane of the nuclear envelope (Kitajima et al, 2001).…”

“…In situ immunolabelling assays revealed that the intranuclear viroplasm contains viral structural antigens and may be the site of virion assembly (Kitajima et al, 2001). A number of virions have also been found in 'spokewheel'-like structures inside of extrusion from the nuclear membrane toward the cytoplasm (Chang et al, 1976;Kitajima et al, 1974;Lesemann & Doraiswamy, 1975). Rarely, enveloped virions measuring 100-120676 nm have been found in the endoplasmic reticulum (Chang et al, 1976;Lesemann & Doraiswamy, 1975), but the relationship of these and virions is uncertain.…”

Orchid Biology: Reviews and Perspectives, VIII

“…Geographical distribution: Japan, West Germany (Lesemann and Doraiswamy, 1975a) and Brazil (Kitajima et aI., 1974).…”