Rodlike Particles Associated with Ringspot Symptoms in Several Orchid Species in Brazil

Virus and Phytoplasma Diseases of Passion Fruit

“…Masses of amorphous, finely granular and electron dense material with circular to elliptical profile, without surrounding membrane with diameter ranging from 5-10 mµ occurred also in the cytoplasm. These affects are seen mostly in mesophyll parenchyma and occasionally in epidermal cells but not in the vascular region and are basically similar to what was reported in tissues infected with CitLV (Kitajima et al, 1974, Colariccio et al, 1995.…”

Mechanical Transmission and Structural Features of Coffee Ringspot Virus (CRV)

“…The bacilliform virions present in negatively stained preparations from crude extracts were morphologically similar to in situ CRV particles (CHAGAS 1980) and also to several rhabdoviruses and rhabdovirus-like particles described in literature (FRANCKI 1973, KNUDSON 1973, KITAJIMA and COSTA 1979. In these preparations the inner components of CRV were not observed except when enveloped by an outer coat as part of virions although tubular structures -regarded as inner components -have been found in situ within nuclei of coffee cells from ringspotted tissues, as reported by KITAJIMA and COSTA (1972) and by CHAGAS (1980).…”

“…The bacilliform virions present in negatively stained preparations from crude extracts were morphologically similar to in situ CRV particles (CHAGAS 1980) and also to several rhabdoviruses and rhabdovirus-like particles described in literature (FRANCKI 1973, KNUDSON 1973, KITAJIMA and COSTA 1979. In these preparations the inner components of CRV were not observed except when enveloped by an outer coat as part of virions although tubular structures -regarded as inner components -have been found in situ within nuclei of coffee cells from ringspotted tissues, as reported by KITAJIMA and COSTA (1972) and by CHAGAS (1980). Such structures showed intracellular behaviour and morphology comparable to the particles associated with citrus leprosis (KITAJIMA et al 1972) and with some orchid diseases (PETZOLD 1971, DuvEL and PETERS 1971, LESEMANN and BEGTRUP 1971, KITAJIMA et al 1974, LESEMANN and DORAISWAMY 1975, Doi et al 1977.…”

“…In Brazil, the findings of KITAJIMA and COSTA (1972) supported the viral assumption of coffee ringspot. They detected bacilliform structures -similar to the inner components of the rhabdovirus -in ringspotted foliar tissues.…”

Detecção do vírus da leprose dos citros nos tecidos da planta infectada e do ácaro vetor Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae)

“…A principal característica destes vírus é a formação de um viroplasma elétron-transparente localizado no núcleo da célula hospedeira . Os vírus do tipo nuclear foram encontrados pela primeira vez em orquídeas infectadas pelo vírus da mancha da orquídea (Orchid fleck virus -OFV) (DOI et al, 1969(DOI et al, , 1977KITAJIMA et al, 1974aKITAJIMA et al, , 2003 e, posteriormente, em vários casos como em alguns isolados de leprose dos citros do tipo nuclear (Citrus leprosis virus nuclear typeCiLVC-N), mancha anular do cafeeiro (Coffe ringspot virus -CoRSV), mancha clorótica do Clerodendrum (Clerodendrum ringspot virus -ClRSV), mancha clorótica de Solanum violaefolium (Solanum violaefolium chlorotic spot virus -SvCSV), mancha clorótica do Hibiscus (Hibiscus chlorotic spot virus -HCSV), mancha anular do Malvaviscus (Malvaviscus ringspot virus -MRSV), entre outros .…”