Virus and Phytoplasma Diseases of Passion Fruit

Singh, Surinder

doi:10.1007/1-4020-2607-2_8

“…are known to be transmitted by seeds (Manicom et al, 2003;Fischer and Rezende, 2008). Passion fruit is at risk of a number of viruses (Singh, 2004;Fischer and Rezende, 2008), and in Japan, where vines are replanted every one or two years, the risk of virus dissemination through propagation is higher than that of perennial fruit trees, which are replanted every decade or so.…”

Section: Introductionmentioning

confidence: 99%

<i>In Vivo</i> Micrografting to Eliminate Passiflora Latent Virus from Infected Passion Fruit Plants

Ogata

¹

,

Yamanaka

²

2021

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An in vivo micrografting method was developed to obtain Passiflora latent virus (PLV)-free passion fruit from infected plants. The scion length required to eliminate PLV was ≤ 2 mm. The method required no aseptic handling and the procedure was relatively simple, and resulted in more than 10 micrografts in one hour. Moreover, no special equipment or procedures were necessary, allowing it to be conducted at an individual farm level. Rapid growth of the scion after grafting was also observed due to the use of fully established seedlings as rootstock. Leaf samples for analysis of PLV infection could therefore be obtained about two months after grafting with fruit harvest possible about four months later. In vivo micrografting was conducted with a scion length of 0.5-1 mm from September to November to determine the optimal air temperature conditions. The graft success rate increased from 18% to 58% with a decrease in the average air temperature from September (28.6°C) to November (23.3°C), although there was no significant difference in PLV-free rates between months (73% to 80%). Accordingly, in vivo micrografting of passion fruit is not recommended under high air temperature conditions. The effect of scion shoot storage conditions was also examined, revealing that in vivo micrografting using shoots stored for one day could be performed without difficulty, whereas the rate of graft success and PLV-free rate were close to those obtained using shoots selected less than 30 min before. These findings suggest that with this method, virus-free plants can be obtained using PLV-infected shoots selected in the field one day earlier. In conclusion, our in vivo micrografting technique is useful to eliminate PLV from infected plants; it is simple and leads to rapid growth of the scion after grafting.

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“…In China, passion fruit orchards are mainly located in the southern part of China such as Guangxi and Fujian provinces. However, the production of passion fruit is negatively affected by various plant diseases and insect pests, especially viruses (Amata, 2011;Singh, 2004). It is documented that passion fruits are susceptible to infection of more than 25 different viruses (Baker et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of two recombinant isolates of telosma mosaic virus infecting Passiflora edulis from Fujian Province in China

Xie

¹

,

Gao

²

,

Shen³

et al. 2020

PeerJ

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Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses to passion fruit (Passiflora edulis) production worldwide, including China. In this study, the complete genome sequence (excluding the poly (A) tail) of two TeMV isolates, Fuzhou and Wuyishan, were determined to be 10,050 and 10,057 nucleotides, respectively. Sequence analysis indicated that Fuzhou and Wuyishan isolates share 78–98% nucleotide and 83–99% amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates and PasFru were clustered into a monophyletic clade with high confidences. This indicated that PasFru and the four TeMV isolates should be considered as one potyvirus species. Two recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the first report of TeMV recombinants worldwide.

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“…In 36 China, passion fruit orchards are mainly located in the southern part of China such as Guangxi 37 and Fujian provinces. However, the production of passion fruit is negatively affected by various 38 plant diseases and insect pests, especially viruses (Amata 2011; Singh 2004). It is documented 39 that passion fruits are susceptible to infection of more than 25 different viruses (Baker et al 40 2011).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Molecular characterization of two recombinant isolates of telosma mosaic virus infecting Passiflora edulis from Fujian Province in China (v0.2)"

Wylie¹

2020

0

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Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses to passion fruit (Passiflora edulis) production worldwide, including China. In this study, the complete genome sequence (excluding the poly(A) tail) of two TeMV isolates, Fuzhou and Wuyishan, were determined to be 10,050 and 10, 057 nucleotides, respectively. Sequence analysis indicated that Fuzhou and Wuyishan isolates share 78%-98% nucleotide and 83%-99% amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates and PasFru were clustered into a monophyletic clade with high confidences. This indicated that PasFru and the four TeMV isolates should be considered as one potyvirus species. Two recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the first report of TeMV recombinants worldwide.PeerJ reviewing PDF | Abstract 20 Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses 21 to passion fruit (Passiflora edulis) production worldwide, including China. In this study, the 22 complete genome sequence (excluding the poly(A) tail) of two TeMV isolates, Fuzhou and 23 Wuyishan, were determined to be 10,050 and 10, 057 nucleotides, respectively. Sequence 24 analysis indicated that Fuzhou and Wuyishan isolates share 78%-98% nucleotide and 83%-99% 25 amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new 26 potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates 27 and PasFru were clustered into a monophyletic clade with high confidences. This indicated that 28 PasFru and the four TeMV isolates should be considered as one potyvirus species. Two 29 recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, 30 and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the 31 first report of TeMV recombinants worldwide. 32PeerJ reviewing PDF |

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Virus and Phytoplasma Diseases of Passion Fruit

Cited by 6 publications

References 28 publications

<i>In Vivo</i> Micrografting to Eliminate Passiflora Latent Virus from Infected Passion Fruit Plants

<i>In Vivo</i> Micrografting to Eliminate Passiflora Latent Virus from Infected Passion Fruit Plants

Molecular characterization of two recombinant isolates of telosma mosaic virus infecting Passiflora edulis from Fujian Province in China

Peer Review #1 of "Molecular characterization of two recombinant isolates of telosma mosaic virus infecting Passiflora edulis from Fujian Province in China (v0.2)"

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