Peer Review #1 of "Molecular characterization of two recombinant isolates of telosma mosaic virus infecting Passiflora edulis from Fujian Province in China (v0.2)"

Abstract

Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses to passion fruit (Passiflora edulis) production worldwide, including China. In this study, the complete genome sequence (excluding the poly(A) tail) of two TeMV isolates, Fuzhou and Wuyishan, were determined to be 10,050 and 10, 057 nucleotides, respectively. Sequence analysis indicated that Fuzhou and Wuyishan isolates share 78%-98% nucleotide and 83%-99% amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates and PasFru were clustered into a monophyletic clade with high confidences. This indicated that PasFru and the four TeMV isolates should be considered as one potyvirus species. Two recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the first report of TeMV recombinants worldwide.PeerJ reviewing PDF | Abstract 20 Telosma mosaic virus (TeMV) is an important plant virus causing considerable economic losses 21 to passion fruit (Passiflora edulis) production worldwide, including China. In this study, the 22 complete genome sequence (excluding the poly(A) tail) of two TeMV isolates, Fuzhou and 23 Wuyishan, were determined to be 10,050 and 10, 057 nucleotides, respectively. Sequence 24 analysis indicated that Fuzhou and Wuyishan isolates share 78%-98% nucleotide and 83%-99% 25 amino acid sequence identities with two TeMV isolates of Hanoi and GX, and a proposed new 26 potyvirus, tentatively named PasFru. Phylogenetic analysis indicated that these TeMV isolates 27 and PasFru were clustered into a monophyletic clade with high confidences. This indicated that 28 PasFru and the four TeMV isolates should be considered as one potyvirus species. Two 29 recombination breakpoints were identified within the CI and NIb genes of the Fuzhou isolate, 30 and also within the P1 gene of the Wuyishan isolate. To the best of our knowledge, this is the 31 first report of TeMV recombinants worldwide. 32PeerJ reviewing PDF |