Structural elucidation of the main water-soluble polysaccharide from Rubus anatolicus roots

Sahragard, Neda; Jahanbin, Kambiz

doi:10.1016/j.carbpol.2017.08.042

Cited by 29 publications

(8 citation statements)

References 43 publications

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“…The Mw of HCP-1a was determined by HPGPC, which has been shown to be an effective method [ 21 ]. As shown in Table 2 , the Mw of HCP-1a was calculated as 33.9 kDa according to the calibration curve.…”

Section: Resultsmentioning

confidence: 99%

“…Bradford method was applied to determine the protein content in polysaccharides applying bovine serum albumin (BSA) as a standard [ 20 ]. Hydroxy biphenyl method was used to determine the uronic acid content in polysaccharides applying bovine D-glucuronic acid as a standard [ 21 ]. Barium chloride–gelatin method was devoted to determine the sulfuric radical content in polysaccharides applying potassium sulphate as a standard [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

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Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Lin

Xiong

Zhao

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration (X1), 30 of liquid-to-solid ratio (X2), 56°C of extraction temperature (X3), 200W of ultrasonic power (X4), 10 min of extraction time (X5), and 5 of pH value (X6). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Lin

Xiong

Zhao

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Methanol was repeatedly added and dried to remove trifluoroacetic acid. Finally, the product was acetylated and analyzed by GC (Agilent Technologies lnc., Agilent 7820A; Santa Clara, CA, USA) [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

et al. 2021

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To investigate the structure of Arthrospira platensis polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 106 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.

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“…In order to analyse the structure of the backbone and branch of polysaccharides, PGPIV‐1‐a was subjected to partial acid hydrolysis using the method of Neda et al (Sahragard & Jahanbin, 2017), with some modification. 60 mg of PGPIV‐1‐a was dissolved in 3 mL 0.05 mol L −1 TFA solution, hydrolysed at 80°C for 16 h and then centrifuged to give precipitate PGPIV‐1‐a‐a.…”

Section: Methodsmentioning

confidence: 99%

Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of Platycodon grandiflorum

Shan

Xiong

Liu

et al. 2020

Int J of Food Sci Tech

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Structural elucidation of the main water-soluble polysaccharide from Rubus anatolicus roots

Cited by 29 publications

References 43 publications

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of Platycodon grandiflorum

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