Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Abstract

To investigate the structure of Arthrospira platensis polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 106 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.

Preparation, Characterization, and Bioactivities of Polysaccharide–Nano-Selenium and Selenized Polysaccharides from Acanthopanax senticosus

“…Many studies have found that appropriate molecular modifications or structural modifications can lead to new activities or the further enhancement of the original activities of polysaccharides [31]. The selenization modification of polysaccharides has become a focus of attention.…”

Arthrospira platensis as future food: a review on functional ingredients, bioactivities and application in the food industry

“…However, a small proportion of A. platensis polysaccharides obtained by different extraction methods were monosaccharides (Ai et al ., 2023). Numerous studies have shown that polysaccharides in A. platensis are mainly composed of glucose, mannose and rhamnose; they also contain a small part of monosaccharides such as arabinose, fucose and galactose (Chen et al ., 2020b; Cao et al ., 2021; Cai et al ., 2022a). Monosaccharide compositions vary with growth environments and extraction methods.…”

Ferroptosis Related Immunomodulatory Effect of a Novel Extracellular Polysaccharides from Marine Fungus Aureobasidium melanogenum

“…Interestingly, fungal extracellular polysaccharides as a promising source of nutrients have attracted an increasing focus [ 1 ]. Previous reports revealed that marine fungal extracellular polysaccharides possessed structural diversity and potential biological activities, such as an Aspergillus versicolor extracellular polysaccharide composed of residues of (1→6)-linked α-D-glucopyranose, slightly branched by single α-D-mannopyranose residues [ 2 ], Hansfordia sinuosae extracellular polysaccharide mainly composed of mannose residue possessed antitumor activity [ 3 ], a polysaccharide from Phoma herbarum YS4108 having immunomodulatory effects on T cells and dendritic cells [ 4 ], an extracellular polysaccharide from the mangrove-associated Fusarium oxysporum, and the polysaccharide derived from Arthrospira platensis with antioxidant activity [ 5 , 6 ]. However, more marine fungal polysaccharides still need to be investigated to understand their structural features and mechanisms of action in depth.…”