Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of <i>Platycodon grandiflorum</i>

Shan, Shan; Xiong, Yi; Liu, Mengyao; Zeng, Deyong; Song, Chen; Baranenko, Denis; Cheng, Dansong; Lu, Weihong

doi:10.1111/ijfs.14846

Cited by 14 publications

(5 citation statements)

References 47 publications

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“…The alcohol extract of P. grandiflorum contains saponins, flavonoids, polysaccharides, polyphenol, and other components 34 . A neutral heteropolysaccharide isolated from P. grandiflorum significantly stimulated the proliferation of RAW264.7 cells in the range of 100 μg/mL to 800 μg/mL (P < 0.05), enhanced its phagocytic ability and increased in a dose-dependent manner 35 . Ethanol extract and fermented extract of P. grandiflorum 17 significantly reduced NO concentration in LPS-induced RAW 264.7 cells in a dose-dependent manner (P < 0.05) 36 .…”

Section: Discussionmentioning

confidence: 93%

Quality control of Platycodon grandiflorum (Jacq.) A. DC. based on value chains and food chain analysis

Jiang,

Niu,

Chen

et al. 2023

Sci Rep

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Platycodon grandiflorum (Jacq.) A. DC. has been proposed as a medicine and food homology, thus playing an important role in disease prevention and health promotion, with great potential for research and value in clinical application. We aimed to analyze stakeholders' production behavior and financial performance from a value chain (VC) perspective and provide a basis for improving the quality of P. grandiflorum and the interests of stakeholders. P. grandiflorum collected from different producing areas were chemically analyzed, and the quality of platycodin D was evaluated. Rstudio3.6.0 was used to analyze the correlation between total platycodins (as platycodin D, platycoside E, and platycodin D3) and platycodin D in P. grandiflorum, providing the basis for quality control of P. grandiflorum. In addition, we studied the anti-inflammatory and anti-cancer activities of P. grandiflorum extract under different links. Based on the food chain energy pyramid, the transfer efficiency of active components of P. grandiflorum in different links was studied. Accordingly, 10 different types of VCs were determined in producing P. grandiflorum. Our results show that vertical coordination has led to a more consistent traceability system and strict regulation of supply chains.

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Section: Discussionmentioning

confidence: 93%

Quality control of Platycodon grandiflorum (Jacq.) A. DC. based on value chains and food chain analysis

Jiang,

Niu,

Chen

et al. 2023

Sci Rep

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“…Moreover, the signal peak at 3.70/76.07 ppm was attributed to the correlation of H-1/C-4, confirming the existence of the →4)-α-Glcp-(1→ residue, and the signal peaks at 4.10/103.98 ppm, 3.67/103.98 ppm, and 3.57/104.98 ppm were ascribed to the correlations of H-4/C-2, H-1/C-2, and H-6/C-2, respectively, validating the presence of the Fruf-(2→ and →6-)-β-Fruf-(2→ residues. By employing the same methodology and comparing it with previously published NMR data [ 13 , 14 , 15 , 16 , 17 , 18 , 19 ], the chemical shifts of other residues’ protons and carbons were sequentially deduced, as detailed in Table 3 . Based on these findings, a plausible structure for DT1-0 is proposed in Figure 9 .…”

Section: Resultsmentioning

confidence: 99%

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Dai,

Xu,

Zhou

et al. 2024

Polymers

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In this study, a Bacillus halotolerans (B. halotolerans) strain DT1 capable of producing exopolysaccharides (EPS) was isolated from dried cabbages of Tianjin, a local fermented vegetable product. Three distinct polysaccharide fractions were isolated from the fermentation broth of DT1, namely, DT1-0, DT1-2, and DT1-5. The structural composition and properties of these fractions were investigated. The predominant EPS, DT1-0, was identified as a novel heteropolysaccharide composed of fructose and glucose with branched structures. The repeating unit was determined to be [4)-α-D-Glcp-(1→6)-α-D-Glcp-(1→6)-β-D-Fruf-(2→6)-β-D-Fruf-(2→6-)-β-D-Fruf-(2→], with fructose and glucose connected by β-(2→1) and α-(1→4) glycosidic linkages between the third fructose and the first glucose, respectively. The molecular weight (Mw) was estimated to be 4.253 × 103 Da. DT1-0 presented a smooth and porous surface structure as observed through SEM and exhibited a water-holding capacity of 504 ± 5.3%, maximum thermal stability at 245 °C, and an oil-holding capacity of 387 ± 1.9% for coconut oil. DT1-2 was identified as a fructooligosaccharide. DT1-5 was characterized as a polysaccharide composed of glucose and fructose. In conclusion, these findings provide substantial support for the further application of B. subtilis strain DT1 and its EPS fractions, DT1-0, DT1-2, and DT1-5, as potential alternatives for functional food additives or ingredients.

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“…In the cur- Macrophages are mononuclear phagocytes that initiate and engage in innate immunological responses, which play an important role in host defense by defending the infection of pathogens and foreign materials (Wang et al, 2020). The natural polysaccharides exert immunostimulatory effects by activating macrophage immune system functions, such as phagocytosis and cytokine secretion (Shan et al, 2020). The phagocytic ability is one of the most crucial parameters of macrophage activation.…”

Section: Discussionmentioning

confidence: 99%

Effect of steam explosion pretreatment on polysaccharide isolated from Poria cocos : Structure and immunostimulatory activity

Liang

Zhao

Xie

et al. 2022

Journal of Food Biochemistry

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This study aimed to examine the effects of steam explosion (SE) pretreatment on the structural characteristics and immunostimulatory activity of polysaccharide from Poria cocos. Results showed that the average molecular weights of native polysaccharide (PCP) and SE-pretreated polysaccharide (SEPCP) were 18.67 and 6.52 kDa, respectively. PCP and SEPCP shared the same profiles of monosaccharides (mannose, glucose, galactose, and fucose) in different composition ratios, that is, PCP in a molar

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Structural characterization and immunomodulatory activity of a new polysaccharide isolated from the radix of Platycodon grandiflorum

Abstract: In this study, a novel polysaccharide fraction (PGPⅣ‐1‐a) was isolated from Platycodon grandiflorum, and its structure properties and immunomodulatory activity were investigated.

Cited by 14 publications

References 47 publications

Quality control of Platycodon grandiflorum (Jacq.) A. DC. based on value chains and food chain analysis

Quality control of Platycodon grandiflorum (Jacq.) A. DC. based on value chains and food chain analysis

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Effect of steam explosion pretreatment on polysaccharide isolated from Poria cocos : Structure and immunostimulatory activity

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