Effect of steam explosion pretreatment on polysaccharide isolated from
            <i>Poria cocos</i>
            : Structure and immunostimulatory activity

Liang, Jiehua; Zhao, Minhao; Xie, Shuai; Peng, Dong; An, Miaoqing; Chen, Yang; Li, Pan; Du, Bing

doi:10.1111/jfbc.14355

Cited by 12 publications

(10 citation statements)

References 43 publications

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“…This resonance pattern serves to confirm the presence of uronic acids within the molecular composition of CPTM-P1; a deduction further substantiated by the findings of HPLC-MS analysis. It is well known that the type of glycosidic linkage plays a crucial role in determining the immunomodulatory activity of polysaccharides [ 44 ]. For example, the anti-inflammatory efficacy of Poria cocos polysaccharides has been linked to a 1,3-α-d-Galp backbone and 1,6-α-d-Galp branches [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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Polysaccharides extracted from Taxus media hrough an aqueous method were further refined by removing proteins via the Sevag technique and purified by dialysis. The separation of these polysaccharides was accomplished using a DEAE-cellulose chromatog-raphy column, yielding two distinct fractions, named CPTM-P1 and CPTM-P2. Notably, CPTM-P1 emerged as the primary polysaccharide component within Taxus media. Consequently, a comprehensive analysis focusing exclusively on CPTM-P1 was undertaken. The molecular weight of CPTM-P1 was established through gel permeation chromatography (GPC), and its monosaccharide composition was deciphered using HPLC-MS. The structure was further elucidated through nuclear magnetic resonance (NMR) spectroscopy. The molecular weight of CPTM-P1 was determined to be 968.7 kDa. The monosaccharide composition consisted of galactose (Gal), arabinose (Ara), galacturonic acid (Gal-UA), glucose (Glc), rhamnose (Rha), xylose (Xyl), mannose (Man), fucose (Fuc), glucuronic acid (Glc-UA), and ribose (Rib). The proportional distribution of these components was 30.53%, 22.00%, 5.63%, 11.67%, 11.93%, 1.69%, 8.50%, 1.23%, 5.63%, and 1.17%, respectively. This confirmed CPTM-P1 as an acidic heteropolysaccharide with a glycuronic acid backbone. Moreover, CPTM-P1 showed immunoenhancing properties, effectively augmenting the secretion of nitric oxide and cytokines (TNF-α, IL-1β, and IL-6). Additionally, it significantly enhances the phagocytic capacity of RAW264.7 cells. These findings underscore the potential application of these polysaccharides in functional foods and pharmaceuticals, providing a solid scientific basis for further exploration and utilization of Taxus media polysaccharides.

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Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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“…In recent years, various methods have been developed to extract bioactive polysaccharides, including physicochemical (SE-assisted extraction, hydrothermal, fiber expansion with ammonia), chemical (dilute acids, alkalis, ionic liquids, enzymatic, oxidizing agents, organic solvents), physical (grinding, irradiation, extrusion) and biological (fermentation) [11,12] techniques. Hot water extraction is the most commonly used of these extraction methods, with the advantages of mild conditions and simple operation, but this method often results in low polysaccharide yield, high energy consumption and low biological activity of the extracted polysaccharide [13].…”

Section: Introductionmentioning

confidence: 99%

Steam Explosion-Assisted Extraction of Polysaccharides from Pleurotus eryngii and Its Influence on Structural Characteristics and Antioxidant Activity

Qiu,

Zheng,

Dai

et al. 2024

Foods

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Pleurotus eryngii (PE) has been sought after for its various health benefits and high content of phenolic compounds. This study explored the feasibility of steam explosion (SE)-assisted extraction of polysaccharides with high antioxidant capacities from PE. An orthogonal experimental design (OED) was used to optimize the SE-assisted extraction of PE. The influence of the optimized SE-assisted extraction on the physicochemical properties of PE polysaccharides was determined by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), monosaccharide compositional analysis and antioxidant capacity assays. Under optimal SE conditions, SE-assisted extraction increased the polysaccharide yield by 138% compared to extraction without SE-assistance. In addition, SEM demonstrated that SE-assisted extraction markedly altered the spatial structure of Pleurotus eryngii polysaccharides (PEP), and monosaccharide compositional analysis revealed that this pretreatment significantly increased the proportions of some monosaccharides, such as glucose, rhamnose and arabinose, in the isolated PEP. FTIR spectra indicated no change in the major chemical functional groups of PEP. PEP extracted by SE-assisted extraction had significantly increased free radical scavenging and antioxidant capacities. In conclusion, SE-assisted extraction appears to be a novel polysaccharide extraction technology, which markedly increases extraction yields and efficiency and can increase the biological activity of polysaccharide extracts.

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“…Poria cocos is an edible mushroom and has been used for medicine for a long history owing to its specific characterization and biological activities ( Zhu et al, 2019 ; Liang et al, 2022 ; Zheng et al, 2022 ). Poria cocos is composed of multiple chemical composition, including triterpenes, polysaccharides, steroids, amino acids, choline, and histidine.…”

Section: Introductionmentioning

confidence: 99%

Poria cocos polysaccharide—functionalized graphene oxide nanosheet induces efficient cancer immunotherapy in mice

Yang

Dong

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Tumor vaccines that induce robust humoral and cellular immune responses have attracted tremendous interest for cancer immunotherapy. Despite the tremendous potential of tumor vaccines as an effective approach for cancer treatment and prevention, a major challenge in achieving sustained antitumor immunity is inefficient antigen delivery to secondary lymphoid organs, even with adjuvant aid.Methods: Herein, we present antigen/adjuvant integrated nanocomplexes termed nsGO/PCP/OVA by employing graphene oxide nanosheet (nsGO) as antigen nanocarriers loaded with model antigen ovalbumin (OVA) and adjuvant, Poria cocos polysaccharides (PCP). We evaluated the efficacy of nsGO/PCP/OVA in activating antigen-specific humoral as well as cellular immune responses and consequent tumor prevention and rejection in vivo.Results: The optimally formed nsGO/PCP/OVA was approximately 120–150 nm in diameter with a uniform size distribution. Nanoparticles can be effectively engulfed by dendritic cells (DCs) through receptor-mediated endocytosis, induced the maturation of DCs and improved the delivery efficiency both in vitro and in vivo. The nsGO/PCP/OVA nanoparticles also induced a significant enhancement of OVA antigen-specific Th1 and Th2 immune responses in vivo. In addition, vaccination with nsGO/PCP/OVA not only significantly suppressed tumor growth in prophylactic treatments, but also achieved a therapeutic effect in inhibiting the growth of already-established tumors.Conclusion: Therefore, this potent nanovaccine platform with nanocarrier nsGO and PCP as adjuvants provides a promising strategy for boosting anti-tumor immunity for cancer immunotherapy.

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Effect of steam explosion pretreatment on polysaccharide isolated from Poria cocos : Structure and immunostimulatory activity

Cited by 12 publications

References 43 publications

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Steam Explosion-Assisted Extraction of Polysaccharides from Pleurotus eryngii and Its Influence on Structural Characteristics and Antioxidant Activity

Poria cocos polysaccharide—functionalized graphene oxide nanosheet induces efficient cancer immunotherapy in mice

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