Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides fromHedyotis corymbosa

Abstract

In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration (X1), 30 of liquid-to-solid ratio (X2), 56°C of extraction temperature (X3), 200W of ultrasonic power (X4), 10 min of extraction time (X5), and 5 of pH value (X6). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.

Fatty Acid Derivative of Methanol Extract of Oldenlandia corymbosa: A Potential Compound against K. pneuminiae and MCF-cell Lines

“…Previous studies determined the medicinal properties of various extracts of Oldenlandia corymbosa plant (21)(22)(23). The present study shows that the compound from Oldenlandia corymbosa has significant antioxidant as well as anticancer activity.…”

Immunomodulatory Activity of Natural Polysaccharides in Combating Covid -19, Cancer, Inflammatory Disorders: A Review

“…They are the homopolymers of D-glucose are the most abundant carbohydrates in the cellular walls of several microorganisms, such as mushrooms, yeast, algae, bacteria, lichens, and plants, and exhibit immunomodulatory, antitumor, and antiinflammatory activities 8 . The other components are Mannanoligosaccharides which contains polymers of mannose that are obtained from yeast cell walls, and are located on the outer surface of yeast cell walls attached to β-glucans of the inner matrix via β- (1,6) and β-(1,3) glycosidic linkages. The structures are given in Fig.…”

“…Polysaccharides belong to the category of biomacromolecules and are found in various traditional herbs, marine organisms or microorganisms. They exhibited a variety of biological activities such as immunomodulatory, antioxidant, antiaging, antitumor and anti-inflammatory activities 1,2 . Many studies have been carried out to study the bioactivity of these plant polysaccharides.…”

Simultaneous determination and quality evaluation of 16 compounds in Ligustri Lucidi Fructus covering different regions and processed products using ultra‐high‐performance liquid chromatography‐mass spectrometry

“…Flavonoids such as apigenin, luteolin, and quercetin are the main components of lipid‐lowering effects in LLF (Qiu et al, 2017) and are also studied due to their various pharmacological effects. In addition, plant polysaccharides found commonly in traditional medicinal herbs have been studied for many years to our knowledge; ganoderan, jujube polysaccharides (Ji et al, 2017), grifola polysaccharide, persimmon polysaccharides (Zhang et al, 2011), panaxan, and pachymaran exhibit a wide range of pharmacological activities, including antioxidant, immune regulation, antitumor, hypoglycemic, hypolipidemic, and antiaging activities (Lin et al, 2018; Shi, 2016; Wang et al, 2019). LLF polysaccharide has gained researchers' attention in recent years, and it has been reported that it is composed of glucose, rhamnose, and arabinose (Zhan et al, 2019) and exhibits immunomodulatory and antioxidant activities (Liu et al, 2020).…”