Structural and ultrastructural study of the rabbit testes exposed to carbamate insecticide

Abstract

The present study was conducted to investigate the impact of carbamate insecticide - bendiocarb on the testicular structure of adult rabbits. Bendiocarb was perorally administered daily for 10 and 30 days, at a dose 5 mg/kg of body weight. After the histological sampling the tissues were investigated and compared with control. After the bendiocarb administration the absolute and relative testicular weight decreased significantly (P < 0.001) in both time periods. The testicular parenchyma showed structural changes such the sloughing of developing sex cells, occurrence of vacuoles within Sertoli cells and inside various spermatogenic cells. The interstitial Leydig cells were smaller than their control counterpart and possessed shrivelled nuclei and strongly vacuolar dark cytoplasm. The rate of changes was directly proportional on duration of the experiment. The ultrastructural examination proved presence of various cellular defects across the germinal epithelium as well as within the interstitial Leydig cells in both experimental periods. Morphometric analysis manifested decrease in diameters of seminiferous tubules, increase of the diameters of the tubular lumina due to reduction of height of the seminiferous epithelium. Results of this study show distinct negative effects of bendiocarb on structure of rabbit testes.

Ultrastructural changes in the rabbit liver induced by carbamate insecticide bendiocarb

“…Carbamate also induced changes in rabbit testes. Almasiova et al [8] observed sloughing of developing sex cells in testicular parenchyma and vacuoles within Sertoli cells. The intestinal Leydig cells were smaller with shrivelled nuclei and strongly vacuolar dark cytoplasm.…”

Morphometric alterations, steatosis, fibrosis and active caspase‐3 detection in carbamate bendiocarb treated rabbit liver

“…Symptoms of bendiocarb poisoning include weakness, blurred vision, headache, nausea, abdominal cramps, chest discomfort, miosis, sweating, muscle tremors and incoordination, decreased pulse, low blood pressure, heart irregularities, giddiness, confusion, slurred speech, and loss of reflexes. Respiratory failure resulting from inhibition of the central respiratory drive and bronchospasms coupled with depolarizing blockade at neuromuscular junctions‐diaphragm and intercostals (Krieger, ; Almasiova et al, ). Many studies proved that bendiocarb increases incidence of lymphoreticular tumours, such as lymphosarcomas, reticulosarcomas, lymphoid, and myeloid leukaemia (Dorko et al, ).…”

Structural and ultrastructural study of the rabbit kidney exposed to carbamate insecticide

“…Neurotoxicity as the main impact of carbamates as well as alterations of homeostasis (Sobekova et al 2009;Capcarova et al 2010;Mojzisova et al 2012) or other important organs such as thymus (Flesarova et al 2007), lymphoid tissue , liver (Holovska et al 2011;Pollakova et al 2012), spleen (Petrovova et al 2011), and testes (Almasiova et al 2012) have been studied in great detail.…”