Structural and ultrastructural study of the rabbit kidney exposed to carbamate insecticide

Abstract

The aim of the present study was to determine the influence of orally administered insecticide bendiocarb on the structure and ultrastructure of the kidney parenchyma in rabbits. Bendiocarb in the form of capsules (96% Bendiocarb, Bayer), at a dose of 5 mg/kg of body weight was fed daily for 3 days. After sampling, kidney sections of experimental and control animals were evaluated. Under a light and electron microscope the diffuse degenerative changes in kidney cortex and medulla were noted. Light microscopy revealed that the renal corpuscles had normal structure, but other nephron components and the collecting ducts were invariably changed. The epithelial cells inside the proximal and distal tubules and collecting ducts possessed increased quantity of cytoplasmic vacuoles and some tubular sections showed cellular sloughing and necrotization. The cells within the thin limbs of the Henley's loops had normal histological structure except for sporadic necrotizing cells within some segments. The ultrastructural evaluation showed extensive cytoplasmic vacuolisation and degenerative changes, such as mitochondrial swelling and shortening of basal infoldings within proximal and distal tubules, and microvilli reduction within proximal tubules. Cells of the collecting tubules exhibited a higher number of vacuoles and some cells had apparently reduced organelles. The cells of the thin limbs of the Henle's loop showed more vacuolised cytoplasm, some tubular sections revealed cellular detachment between the adjacent epithelial cells and rare necrotising epithelial cells were observed. The described findings addressed in the present study indicate an adverse effect of bendiocarb on the kidney parenchyma in rabbits.

Effect of the bendiocarb on the ultrastructure of rabbit skeletal muscle

“…Bendiocarb was shown to alter homeostasis (Capcarova et al 2010;Mojzisova et al 2012) and induce morphological changes in different organs such as the thymus, kidneys, and liver (Flesarova et al 2007;Almášiová et al 2014;Holovska et al 2014). In agreement with previously published studies, it can be concluded that bendiocarb adversely affected the structure of rabbit tissues and organs.…”

Simultaneous poisoning of 48 birds of prey – bendiocarb determination with the use of UHPLC-ESI-MS/MS method in fatal case from Eastern Europe

“…such as mitochondrial swelling and shortening of basal infoldings within proximal and distal tubules. Moreover, within Henle's loop, vacuolized cytoplasm was noticed and tubular sections revealed cellular detachment between the adjacent epithelial cells and rare necrotizing epithelial cells [26]. Kidney function in bendiocarb-treated rats was also compromised and was characterized as a disruption of antioxidative barrier (CAT, SOD, GPx) together with an increased level of malondialdehyde, creatine, urea, and uric acid.…”

“…The animals developed convulsions and died within 30 minutes to one hour after ingestion of bendiocarb-soaked pieces of bread [25]. Acute bendiocarb intoxication causes tachycardia, pupil dilation, muscle contraction, excessive salivation, nausea, vomiting, as well as ataxia, seizures and coma [26,27,28]. The symptoms after intoxication include paralysis of muscles of the respiratory system with intense constriction of the pulmonary alveolus [20].…”