“…3,6,7 This bioreactor has applied 5%-10% elongation with 0.2-1 Hz frequency to 3D scaffolds including polyurethane nanofibrous scaffolds, 8 and fibrin, 9 and collagen gels. 10 Tensile stress of 3%-20% magnitude modulated expression of bone-related molecules such as collagen, 7,11 osteocalcin (OCN), 1,7 osteopontin (OPN), 1,7 matrix metalloproteinase-13 (MMP-13), 12 osteoprotegerin (OPG), 6 prostaglandin E 2 (PGE 2 ), 13 cyclooxygenase-2 (COX-2), 14 vascular endothelial growth factor (VEGF), 1,15 and transforming growth factor-b1 (TGF-b1). 16 Other external stimulating factors for bone regeneration are osteoinductive growth factors (GFs) such as TGF, bone morphogenetic protein (BMP), and platelet-derived GF.…”