Periprosthetic strain magnitude-dependent upregulation of type I collagen synthesis in human osteoblasts through an ERK1/2 pathway

Zhu, Junfeng; Wang, Chengtao; Peng, Xiaochun; Zhang, Xianlong

doi:10.1007/s00264-009-0735-z

Cited by 8 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with previous work in both 2D and 3D, showing that stretch acutely activates a number of protein kinases in mesenchymal cells. [10][11][12][13][14] The decision to concentrate solely on ERK1/2 activation was based on the important role that ERK1/2 plays in the stretch-activated increase in ColIA1 protein and gene expression 14,25,26 and the quality of the ERK1/2 antibodies available for analysis. In an effort to elucidate the activation profile of ERK1/2 in the ligament constructs, constructs were subjected to cyclic uniaxial stretch for varying times from 1 min to 1 h. Maximal activation of ERK1/2 was seen to occur at 10 min of stretch (Figs.…”

Section: Discussionmentioning

confidence: 99%

Optimizing an Intermittent Stretch Paradigm Using ERK1/2 Phosphorylation Results in Increased Collagen Synthesis in Engineered Ligaments

Paxton

Hagerty

Andrick

et al. 2012

Tissue Engineering Part A

View full text Add to dashboard Cite

Dynamic mechanical input is believed to play a critical role in the development of functional musculoskeletal tissues. To study this phenomenon, cyclic uniaxial mechanical stretch was applied to engineered ligaments using a custom-built bioreactor and the effects of different stretch frequency, amplitude, and duration were determined. Stretch acutely increased the phosphorylation of p38 (3.5 -0.74-fold), S6K1 (3.9 -0.19-fold), and ERK1/2 (2.45 -0.32-fold). The phosphorylation of ERK1/2 was dependent on time, rather than on frequency or amplitude, within these constructs. ERK1/2 phosphorylation was similar following stretch at frequencies from 0.1 to 1 Hz and amplitudes from 2.5% to 15%, whereas phosphorylation reached maximal levels at 10 min of stretch and returned toward basal within 60 min of stretch. Following a single 10-min bout of cyclic stretch, the cells remained refractory to a second stretch for up to 6 h. Using the phosphorylation of ERK1/2 as a guide, the optimum stretch paradigm was hypothesized to be 10 min of stretch at 2.5% of resting length repeated every 6 h. Consistent with this hypothesis, 7 days of stretch using this optimized intermittent stretch program increased the collagen content of the grafts more than a continuous stretch program (CTL = 3.1% -0.44%; CONT = 4.8% -0.30%; and INT = 5.9% -0.56%). These results suggest that short infrequent bouts of loading are optimal for improving engineered tendon and ligament physiology.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimizing an Intermittent Stretch Paradigm Using ERK1/2 Phosphorylation Results in Increased Collagen Synthesis in Engineered Ligaments

Paxton

Hagerty

Andrick

et al. 2012

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…These cells were previously characterized as authentic osteoblasts. 24 The cells were seeded in 25-cm 2 flasks and incubated at 37°C in 5% CO 2 with DMEM/F12 (Gibco) supplemented with 0.3 mg/mL G418 (Sigma) and 10% fetal bovine serum (HyClone). Then, the cells were propagated as described by the manufacturer.…”

Section: Bacteria Human Osteoblasts and Culture Conditionsmentioning

confidence: 99%

Staphylococcus aureus regulates secretion of interleukin-6 and monocyte chemoattractant protein-1 through activation of nuclear factor kappaB signaling pathway in human osteoblasts

Ning¹,

Zhang²,

Guo³

et al. 2011

Braz J Infect Dis

Self Cite

View full text Add to dashboard Cite

Objective: Activation of nuclear factor kappaB by diverse bacteria regulates the secretion of chemokines and cytokines. Staphylococcus aureus (S. aureus)-infected osteoblasts can significantly increase the secretion of interleukin-6 and monocyte chemoattractant protein-1. The aim of this study was to investigate whether S. aureus can activate nuclear factor kappaB in human osteoblasts, and whether the activation of nuclear factor kappaB by S. aureus regulates the secretion of interleukin-6 and monocyte chemoattractant protein-1. Methods: Immunoblot and electrophoretic mobility shift assay were used to detect the degradation of IκBa and activation of nuclear factor kappaB in human osteoblasts in response to S. aureus, respectively. Enzyme-linked immunosorbent assay was used to measure the secretion of interleukin-6 and monocyte chemoattractant protein-1 in the supernatants. Lastly, carbobenzoxyl-l-leucinyl-l-leucinyl-l-leucinal, an inhibitor of the nuclear factor kappaB, was used to determine if activation of nuclear factor kappaB by S. aureus in human osteoblasts regulates the secretions of interleukin-6 and monocyte chemoattractant protein-1. Results: Our results for the first time demonstrated that S. aureus can induce the degradation of IκBa and activation of nuclear factor kappaB in human osteoblasts in a time and dose-dependent manner. In addition, inhibition of nuclear factor kappaB by carbobenzoxyl-l-leucinyl-l-leucinyl-l-leucinal suppressed the secretion of interleukin-6 and monocyte chemoattractant protein-1 in the supernatants of S. aureus-infected human osteoblasts in a dose-dependent manner. Conclusions: These findings suggest that S. aureus can activate nuclear factor kappaB in human osteoblasts, and subsequently regulate the secretion of interleukin-6 and monocyte chemoattractant protein-1. The nuclear factor kappaB transcription factor regulates a number of genes involved in a wide variety of biological processes. Further study of the effects of nuclear factor kappaB activation on S. aureus-infected human osteoblast may provide us new insights into discovery of the immune mechanisms in osteomyelitis.

show abstract

“…SV40 human osteoblasts (SV40 hOBs) were obtained from the American Type Culture Collection. These cells had previously been characterized as authentic osteoblasts 16 . The cells were seeded in 25‐cm 2 flasks and incubated at 37°C in 5% CO 2 with DMEM/F12 (Gibco, Gaithersburg, MD, USA) supplemented with 0.3 mg/mL G418 (Sigma, St Louis, MO, USA) and 10% fetal bovine serum (HyClone, Logan, UT, USA).…”

Section: Methodsmentioning

confidence: 99%

The effect of Staphylococcus aureus on apoptosis of cultured human osteoblasts

Ning

Zhang

et al. 2011

Orthopaedic Surgery

View full text Add to dashboard Cite

show abstract

Periprosthetic strain magnitude-dependent upregulation of type I collagen synthesis in human osteoblasts through an ERK1/2 pathway

Cited by 8 publications

References 32 publications

Optimizing an Intermittent Stretch Paradigm Using ERK1/2 Phosphorylation Results in Increased Collagen Synthesis in Engineered Ligaments

Optimizing an Intermittent Stretch Paradigm Using ERK1/2 Phosphorylation Results in Increased Collagen Synthesis in Engineered Ligaments

Staphylococcus aureus regulates secretion of interleukin-6 and monocyte chemoattractant protein-1 through activation of nuclear factor kappaB signaling pathway in human osteoblasts

The effect of Staphylococcus aureus on apoptosis of cultured human osteoblasts

Contact Info

Product

Resources

About