Response of a Preosteoblastic Cell Line to Cyclic Tensile Stress Conditioning and Growth Factors for Bone Tissue Engineering

Chung, Eunna; Rylander, Marissa Nichole

doi:10.1089/ten.tea.2010.0414

Cited by 11 publications

(14 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Parts of the specimens were subjected to HE [15] and Masson [16] staining, and the ossification results were observed after fixation and embedding.…”

Section: Methodsmentioning

confidence: 99%

Effects of Vascular Endothelial Growth Factor 165 on Bone Tissue Engineering

Feng

Lingling

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

To study the relationship between vascular endothelial growth factor (VEGF) and formation and repair of engineering bone, second-generation bone marrow stromal cells (BMSCs) of New Zealand white rabbits that were separated in vitro were transfected with VEGF 165 gene vectors by adenovirus to detect gene expressions. Transfected BMSCs and β-tricalcium phosphate material were complexed and implanted at the femoral injury sites of the study group (n = 12), and the control group (n = 12) were implanted with engineering bones that were not transfected with VEGF. Femoral recoveries of the two groups were observed on the 15th, 30th, 45th and 60th days, and their vascularization and ossification statuses were observed by immunohistochemical methods. The BMSCs transfected with VEGF highly expressed VEGF genes and excreted VEGF. The two groups both experienced increased vascularization and bone volume after implantation (t = 7.92, P<0.05), and the increases of the study group were significantly higher than those of the control group (t = 6.92, P<0.05). VEGF is clinically applicable because it can accelerate the formation and repair of engineering bone by promoting vascularization and ossification.

show abstract

“…Parts of the specimens were subjected to HE [15] and Masson [16] staining, and the ossification results were observed after fixation and embedding.…”

Section: Methodsmentioning

confidence: 99%

Effects of Vascular Endothelial Growth Factor 165 on Bone Tissue Engineering

Feng

Lingling

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…However, heating (for 8 minutes) alone induced mRNA expression of OCN (3.8 RFI), OPN (1.8 RFI), and OPG (2.1 RFI) genes at 8-hour postheating in our previous study [27]. Furthermore, in our prior work [43], we observed significant OPG secretion in response to 5% tensile stress preconditioning, comparable to the current study employing 3% tension, suggesting that OPG can be upregulated by tension, but not significantly by thermal stress. The discrepancy may be caused by culturing cells on a type I collagen-coated flexible substrate and the use of a different FBS concentration in the osteogenic culture media between these two studies.…”

Section: Resultsmentioning

confidence: 93%

Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells

Chung

Sampson

Rylander

2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Stress conditioning (e.g., thermal, shear, and tensile stress) of bone cells has been shown to enhance healing. However, prior studies have not investigated whether combined stress could synergistically promote bone regeneration. This study explored the impact of combined thermal and tensile stress on the induction of heat shock proteins (HSPs) and bone-related proteins by a murine preosteoblast cell line (MC3T3-E1). Cells were exposed to thermal stress using a water bath (44°C for 4 or 8 minutes) with postheating incubation (37°C for 4 hours) followed by exposure to cyclic strain (equibiaxial 3%, 0.2 Hz, cycle of 10-second tensile stress followed by 10-second rest). Combined thermal stress and tensile stress induced mRNA expression of HSP27 (1.41 relative fold induction (RFI) compared to sham-treated control), HSP70 (5.55 RFI), and osteopontin (1.44 RFI) but suppressed matrix metalloproteinase-9 (0.6 RFI) compared to the control. Combined thermal and tensile stress increased vascular endothelial growth factor (VEGF) secretion into the culture supernatant (1.54-fold increase compared to the control). Therefore, combined thermal and mechanical stress preconditioning can enhance HSP induction and influence protein expression important for bone tissue healing.

show abstract

“…[13][14][15]19,44 Previous studies with human periodontal ligament stem cells, preosteoblasts, and osteoblasts have also reported that tensile strain induces osteogenesis and ECM formation. [56][57][58] We have also previously found that 10% cyclic tensile strain induces the upregulation of proinflammatory cytokine regulators: SOCS3 and IL1RN, and angiogenic factors: FGF2, VEGFA, and MMP2 in hASC undergoing osteogenic differentiation. 19 Findings from the current study indicate that upregulation of VEGF in response to 10% cyclic tensile strain is consistent in both hASC and hMSC.…”

mentioning

confidence: 77%

Cyclic Tensile Strain Enhances Osteogenesis and Angiogenesis in Mesenchymal Stem Cells from Osteoporotic Donors

Charoenpanich

Wall

Tucker

et al. 2014

Tissue Engineering Part A

View full text Add to dashboard Cite

We have shown that the uniaxial cyclic tensile strain of magnitude 10% promotes and enhances osteogenesis of human mesenchymal stem cells (hMSC) and human adipose-derived stem cells (hASC) from normal, nonosteoporotic donors. In the present study, MSC from osteoporotic donors were analyzed for changes in mRNA expression in response to 10% uniaxial tensile strain to identify potential mechanisms underlying the use of this mechanical loading paradigm for prevention and treatment of osteoporosis. Human MSC isolated from three female, postmenopausal osteoporotic donors were analyzed for their responses to mechanical loading using microarray analysis of over 47,000 gene probes. Human MSC were seeded in three-dimensional collagen type I constructs to mimic the organic extracellular matrix of bone and 10% uniaxial cyclic tensile strain was applied to promote osteogenesis. Seventy-nine genes were shown to be regulated within hMSC from osteoporotic donors in response to 10% cyclic tensile strain. Upregulation of six genes were further confirmed with real-time RT-PCR: jun D proto-oncogene (JUND) and plasminogen activator, urokinase receptor (PLAUR), two genes identified as potential key molecules from network analysis; phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD) and wingless-type MMTV integration site family, member 5B (WNT5B), two genes with known importance in bone biology; and, PDZ and LIM domain 4 (PDLIM4) and vascular endothelial growth factor A (VEGFA), two genes that we have previously shown are significantly regulated in hASC in response to this mechanical stimulus. Function analysis indicated that 10% cyclic tensile strain induced expression of genes associated with cell movement, cell proliferation, and tissue development, including development in musculoskeletal and cardiovascular systems. Our results demonstrate that hMSC from aged, osteoporotic donors are capable of enhanced osteogenic differentiation in response to 10% cyclic tensile strain with significant increases in the expression of genes associated with enhanced cell proliferation, musculoskeletal development, and angiogenesis. Surprisingly, cyclic tensile strain of magnitude 10% not only enhanced osteogenesis in hMSC from osteoporotic donors, but also enhanced expression of angiogenic factors. Better understanding and methodologies to promote osteogenesis in hMSC from elderly, osteoporotic donors may greatly facilitate achieving long-term success in bone regeneration and functional bone tissue engineering for this ever-growing patient population.

show abstract

Response of a Preosteoblastic Cell Line to Cyclic Tensile Stress Conditioning and Growth Factors for Bone Tissue Engineering

Cited by 11 publications

References 86 publications

Effects of Vascular Endothelial Growth Factor 165 on Bone Tissue Engineering

Effects of Vascular Endothelial Growth Factor 165 on Bone Tissue Engineering

Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells

Cyclic Tensile Strain Enhances Osteogenesis and Angiogenesis in Mesenchymal Stem Cells from Osteoporotic Donors

Contact Info

Product

Resources

About