STP Position Paper: Ovarian Follicular Counting in the Assessment of Rodent Reproductive Toxicity

Regan, Karen; Cline, J. Mark; Creasy, Dianne M.; Davis, Barbara J.; Foley, George L.; Lanning, Lynda L.; Latendresse, John R.; Makris, Susan L.; Morton, Daniel G.; Rehm, Sabine; Stebbins, K. E.

doi:10.1080/01926230490515355

Cited by 49 publications

(17 citation statements)

References 21 publications

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“…Folliculogenesis is a process that takes place in the ovarian cortex involving antral and pre-antral phases. This study confirms that folliculogenesis can be influenced by xenobiotics, including DMPA 18,19 . Our study expands previous findings that monkeys treated with progestin would undergo apoptosis of ovarian epithelial cells relative to the control group and administration of ethinyl estradiol7 or changes in oxidative stress as the basic mechanisms of cellular apoptosis 20 .…”

Section: Discussionsupporting

confidence: 72%

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Yeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma

Maharani¹

2016

Cukurova Medical Journal (Çukurova Üniversitesi Tıp Fakültesi Dergisi)

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ARAŞTIRMA/RESEARCHCombined effects of infusion of green tea and depotmedroxyprogesterone acetate on the number of granulosa cells and the number and size of ovarian primary follicles: an in vivo study in female ratsYeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma AbstractÖz Purpose: The purpose of the present study was to analyze the effects of green tea infusion on the toxicity of ovaries of female rats treated with the contraceptive depotmedroxyprogesterone acetate (DMPA). Material and Methods: A total of twenty-four female rats were randomly divided into four groups, incuding the control group (no treatment), the DMPA-treated group and the group treated with DMPA and infusion of green tea at various doses (165 and 330 mg/gram of body weight per day). The number of granulosa cells, the number of ovarian follicles and the size of ovarian follicles were subjected to histopathological analysis. Results: The number of granulosa cells did not differ significantly among the study groups. The number of ovarian follicles was significantly higher in the DMPAtreated group than that of the control group. Of doses of 165 and 330 mg/g of body weight of green tea administration, only the low dose decreased the number of ovarian follicles significantly relative to the DMPA-treated group, although it has not yet reached the levels comparable to those of the control group. The size of ovarian follicles did not differ significantly among the study groups. Conclusion: DMPA led to an increased number of ovarian follicles, which was restored by infusion of low doses of green tea. Thus, green tea constitutes an herb that can be combined with administration of DMPA and useful in inhibiting the adverse effects of contraceptives.

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Section: Discussionsupporting

confidence: 72%

“…DMPA is classified as a xenobiotic capable of leading to a reduced number of ovarian follicles and loss of various ovarian cells (oogonia, oocytes and somatic cells) 5 . Histologically, the changes in the ovary in the subjects treated with DMPA include follicular degeneration and atresia 6 .…”

Section: Introductionmentioning

confidence: 99%

Yeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma

Maharani¹

2016

Cukurova Medical Journal (Çukurova Üniversitesi Tıp Fakültesi Dergisi)

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“…Folliculogenesis in the ovary is affected by xenobiotics. Xenobiotics has toxicological effects which may cause loss of oogonia, oocytes, somatic cells and even a significant decline in the number of follicles 17 . Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P < 0.05).…”

Section: Discussionmentioning

confidence: 99%

Rodamin B, Oksidatif Stress Aracılığı ile Ovarian Toksisitesi

Maryanti¹,

Suciati²,

Wahyuni³

et al. 2014

Cukurova Medical Journal

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Purpose: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17β-estradiol and thickness of endometrium. Materials and Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17β-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P < 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P < 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce 17β-estradiol level significantly compared to the control (P < 0.05). The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P < 0.05). Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17β-estradiol which ultimately lowered the thickness of endometrium. Key Words: Dye, toxicity, ovarium, endometrium, oxidative stress. Cukurova Medical JournalBulgular: Dişi sıçanlara farklı dozlarda Rodamin B uygulanması, ovarian MDA seviyelerini kontrollere gore anlamlı şekilde arttırmıştır (P < 0.05). Dişi sıçanlarda rodamin B'nin 2. ve 3. dozları primer, sekonder ve De Graaf folikül sayısını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Dişi sıçanlarda rodamin B'nin 2. ve 3. dozlarının uygulanması 17β-estradiol seviyesini kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Rodamin B uygulanması endometrium kalınlığını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05).Sonuç: Rodamin B uygulamasının, oksidatif stress aracılığı ile ovarian toksisitesini tetiklediği, folikül sayısını azalttığı ve, 17β-Estradiol seviyesini azaltalarak endometriyum kalınlığını düşürdüğü sonucuna varılmıştır.

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“…Several factors that may serve as predictors include age, ovarian volume, number of antral follicles and hormonal markers such as estradiol. A woman with a reduced capacity of ovaries has a high rate of stimulation failure and a high rate of pregnancy failure 5 .Folliculogenesis in the ovary is affected by xenobioticswhich may cause loss of oogonia, oocytes, somatic cells and even a significant decline in the number of follicles 6 . Every substance that induces oxidative stress, an imbalance between prooxidant and antioxidants, can cause some of reproductive diseases such as infertility 7 .Previous studies revealed that subcutaneous administration of MSG (4 and 8 mg/g body weight 6 days) to normal adult male mice caused a significant increase in erythrocyte lipid peroxidation 8 .…”

Section: Introductionmentioning

confidence: 99%

Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite

Rohmawati¹,

Istiananingsih²,

Nurdiana³

et al. 2014

Cukurova Medical Journal

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Purpose: Our study aimed to investigate the combinatorial effect of vitamin C and E on oxidative stress, the number of folicles, and hormonal level in female rats orally exposed to monosodium glutamate (MSG). Materials and Methods:Female, twelve weeks old Wistar rats ingested with of MSG at dose 140 mg/200 gram body weight (bw) with or without combination of vitamin C and E. Twenty five rats were divided into five group (n = 5 each), control group, MSG-treatment group, MSG-treatment + 0.2 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1), MSG-treatment + 0.4 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE2), and MSG-treatment + 0.6 mg/g bw vitamin C + 0.04 IU/g bw vitamin E (MSG + CE1). Analysis of malondialdehyde (MDA) level was done colorimetrically. Analysis of the number of ovarian follicles was done histopathologically with hematoxylin eosin staining. Analysis of 17β-estradiol and follicle stimulating hormone (FSH) levels were done by ELISA technique. Results: There were significantly (P < 0.05) increased ovarium MDA levels and atresia follicle number in groups exposed to MSG compared to non-exposure group. The level of FSH, 17β-estradiol, the number of primary, secondary, de Graaf follicles were significantly lower in MSG-treatment group compared to control group (P < 0.05). The administration of combined vitamin C and E (second and third dose) significantly (P < 0.05) decreased the MDA levels and the number of atresia follicle compared to the MSG-exposed groups, to reach level in control group (P > 0.05). MSG + CE2 and MSG + CE3 significantly increased FSH level, number of primary follicles, compared to MSG-treatment group (P < 0.05), to reach similar level in control group (P > 0.05). All dose of combined vitamin C and E significantly increased 17β-estradiol level and the number of secondary and de Graaf follicles compared to MSG-treatment group (P < 0.05), to reach significantly higher level compared to control group (P < 0.05). Conclusion:The present data suggest that combination of vitamin C and E as in this study inhibited ovarium toxicity caused by MSG treatment. Keywords: glutamate; antioxidant; folliculogenesis; hormone; lipid peroxidation Sadece MSG verilmiş grup ile karşılaştırıldıklarında; C ve E vitaminlerinin tüm dozlarının, 17β-estradiol düzeyi ile sekonder ve graff folikül sayılarını anlamlı olarak arttırdıkları tespit edilmiştir (P < 0.05). C ve E vitaminlerinin verildiği gruplarda, 17β-estradiol düzeyi ile sekonder ve graffolikül sayıları, kontrol grubunun üzerindeki düzeylere ulaşmıştır (P < 0.05).Sonuç: Bulgularımız C ve E vitamin kombinesinin MSG'ya maruz kalmaya bağlı olarak gelişen ovaryum toksisitesini inhibe ettiği sonucunu ortaya koymuştur.

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STP Position Paper: Ovarian Follicular Counting in the Assessment of Rodent Reproductive Toxicity

Cited by 49 publications

References 21 publications

Yeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma

Yeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma

Rodamin B, Oksidatif Stress Aracılığı ile Ovarian Toksisitesi

Vitamin C-E ve Monosodyum Glutamata Bağlı Ovaryan Toksisite

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