Stimulation of Growth Hormone Release by Vasoactive Intestinal Peptide and Peptide PHI in Rat Anterior Pituitary Reaggregates

Denef, Carl; Schramme, Carla; Baes, Myriam

doi:10.1159/000124057

Cited by 41 publications

(15 citation statements)

References 18 publications

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“…The results were in agreement with previous reports on in vitro studies on rats [13]. PHI is reported, however, to induce GH release from aggregated rat pituitary cells in culture only after treatment with dexamethasone [12].…”

Section: Discussionsupporting

confidence: 93%

“…As regards the higher potency in GHRH-stimulated bovine GH release than that obtained with VIP, the present study coincided with reports of previous studies on superfused rat pituitary reaggregated cells [12], and on perifused bovine anterior pituitary tissues [15]. It has been reported that the GH releasing effects of GHRH were maintained longer than that of VIP, suggesting that the somatotrophs might be stimulated to release GH for hours by a short period of GHRH action [15].…”

Section: Discussionsupporting

confidence: 91%

“…after treatment with dexamethasone [12]. Rivier et al failed to observe GH releasing activity of VIP, PHI or glucagon on rat pituitary cells [13].…”

mentioning

confidence: 99%

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The Interactive Effects of VIP, PHI, GHRH and SRIF on the Release of Growth Hormone from Cultured Adenohypophysial Cells in Cattle.

Soliman

Hashizume

Ohashi³

et al. 1995

Endocr J

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

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The Interactive Effects of VIP, PHI, GHRH and SRIF on the Release of Growth Hormone from Cultured Adenohypophysial Cells in Cattle.

Soliman

Hashizume

Ohashi³

et al. 1995

Endocr J

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“…commun. ], have been shown to antagonize each other in cultured pituitary reaggregates [8]. Treatment with DA agonists reduced or suppressed the paradoxical G il response to TRH in constitutionally tall adolescents [12].…”

Section: Discussionmentioning

confidence: 99%

Further Evidence That Thyrotropin-Releasing Hormone Participates in the Regulation of Growth Hormone Secretion in the Rat

Bluet‐Pajot

Durand²,

Drouva

et al. 1986

Neuroendocrinology

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Effects of thyrotropin-releasing hormone (TRH) on growth hormone (GH) secretion were investigated in vivo (on intact or mediobasal hypothalamic lesioned rats tested under either anesthesia or free moving conditions) as well as in vitro (in incubation or perifusion systems of anterior pituitary tissue). The peptide induced a rapid, dose-dependent increase of plasma GH levels in free moving animals bearing an extensive lesion of the mediobasal hypothalamus including the median eminence. Under comparable conditions, TRH was ineffective in intact animals. After chloral hydrate anesthesia a GH response to TRH was recorded in both groups, but lesioned rats exhibited a better responsiveness to all doses tested. In vitro TRH increased GH release from incubated or perifused pituitaries sampled from both intact and lesioned rats in a transient and concentration-dependent manner. A similar effect was obtained with the (3 Me His²) analogue of TRH. These findings indicate that TRH can affect GH secretion at the pituitary level under specific experimental conditions and support the hypothesis that either peripheral hormones or other, still unidentified hypothalamic neurohormones may modulate this effect.

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“…In addition to GHRH and SRIH. other peptidergic mechanisms are also involved in the regulation ofGH: for example, vasoactive intestinal peptide and peptide histidine isoleucine [10] stimulate GH release from cultured rat anterior pituitary cells. Insulin-like GH, GH itself and SRIH reduce the secretory activity of human somatotrophs in vitro [II] and in vivo [12], These effects are either direct ef fects upon the pituitary cells or mediated by inhibitory or stimu latory activity upon suprapituitary GHRH and SRIH release.…”

mentioning

confidence: 99%

Influence of Human Corticotropin-Releasing Hormone and Adrenocorticotropin upon Spontaneous Growth Hormone Secretion

Wiedemann¹,

Bardeleben²,

Holsboer³

1991

Neuroendocrinology

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Hormones of the hypothalamo-pituitary-adrenocortical (HPA-) axis are considered to be of physiological and clinical relevance in regulating spontaneous growth hormone (GH) secretion. To further investigate interdependencies between both systems, we studied the effects of adrenocorticotropin [ACTH(1–24)] and human corticotropin-releasing hormone (h-CRH) upon spontaneous GH secretion in 10 male volunteers. Administration of 1 µg ACTH (1–24), 10 µg h-CRH or saline (control: CTL) every hour from 9.00 to 6.00 p.m. resulted in significant differences of cortisol secretion during the entire observation period (8.00 a.m.-3.00 a.m.) between the three groups (p < 0.001, Friedman two-way ANOVA). Mean area under the time course curve (AUC) values (± SEM) for cortisol expressed as ng × 1,000 × min/ml showed also significant differences between the three treatments from 8.00 a.m. to 3.00 a.m.: CTL 64.0 ± 6.4, ACTH(1–24) 178.5 ± 9.4 (p < 0.01, Wilcoxon test), h-CRH 88.5 ± 5.6 (p < 0.01). The main portion of cortisol was released during daytime from 8.00 a.m. to 11.00 p.m., where the most significant differences in the AUC values emerged: CTL 59.6 ± 5.8, ACTH(1–24) 171.5 ± 8.8 (p < 0.01, Wilcoxon test), h-CRH 80.2 ± 5.1 (p < 0.01). With regard to GH secretion, significant differences became obvious between the three treatments during daytime from 8.00 a.m. to 11.00 p.m. and the sleep-related period from 11.00 p.m. to 3.00 a.m. (p < 0.01 and p < 0.02, Friedman two-way ANOVA). The pulse frequency also differed significantly between the treatments during daytime (p < 0.02). Mean AUC values (± SEM) expressed as ng × min/ml for GH from 8.00 a.m. to 11.00 p.m. were: CTL 2,507 ± 614, ACTH(1–24) 3,419 ± 801 (p < 0.02, Wilcoxon test). AUC values for GH from 11.00 p.m. to 3.00 a.m. were: CTL 2,033 ± 368, ACTH(1–24) 1,136 ± 147 (p < 0.02, Wilcoxon test). Also, the number of GH secretory pulses is increased by stimulation with AOΗ(1–24). Stimulation with h-CRH in the same experimental schedule resulted in allusively similar effects, which were less pronounced. While prolonged exposure of somatotrophs to glucocorticoids results in a reduction of GH secretion, short-term exposition to glucocorticoids as resulting from our present stimulation with ACTH(1–24) enhances the amount of GH released. From our current data, we conclude that HPA hormones modulate the spontaneous GH release pattern. This has implications for the interpretation of neuroen-docrine studies in patients with depression, where exaggerated HPA activity often concurs with elevated GH secretion during daytime and decreased GH surges during sleep or following pharmacological stimuli.

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Stimulation of Growth Hormone Release by Vasoactive Intestinal Peptide and Peptide PHI in Rat Anterior Pituitary Reaggregates

Cited by 41 publications

References 18 publications

The Interactive Effects of VIP, PHI, GHRH and SRIF on the Release of Growth Hormone from Cultured Adenohypophysial Cells in Cattle.

The Interactive Effects of VIP, PHI, GHRH and SRIF on the Release of Growth Hormone from Cultured Adenohypophysial Cells in Cattle.

Further Evidence That Thyrotropin-Releasing Hormone Participates in the Regulation of Growth Hormone Secretion in the Rat

Influence of Human Corticotropin-Releasing Hormone and Adrenocorticotropin upon Spontaneous Growth Hormone Secretion

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